Abstract

This paper discusses the task of true physical quantity reconstruction from experimental data on the basis of fluorescence microscopy images when a specific ion indicator is used for an investigation into ion concentration. Brain cell functional activity in the form of calcium oscillation may be processed as quantitation on the basis of measurements of single-wavelength fluorescence intensity using data about dye loading protocol, excitation, and detection conditions without calibration of every experiment.

© 2012 Optical Society of America

Full Article  |  PDF Article
Related Articles
Imaging of Ca2+ intracellular dynamics with a third-harmonic generation microscope

L. Canioni, S. Rivet, L. Sarger, R. Barille, Pierre Vacher, and Pierre Voisin
Opt. Lett. 26(8) 515-517 (2001)

Photonic explorers based on multifunctional nanoplatforms for biosensing and photodynamic therapy

Yong-Eun Lee Koo, Wenzhe Fan, Hoejin Hah, Hao Xu, Dan Orringer, Brian Ross, Alnawaz Rehemtulla, Martin A. Philbert, and Raoul Kopelman
Appl. Opt. 46(10) 1924-1930 (2007)

Estimating weak ratiometric signals in imaging data. I. Dual-channel data

Josef Broder, Anirban Majumder, Erika Porter, Ganesh Srinivasamoorthy, Charles Keith, James Lauderdale, and Andrew Sornborger
J. Opt. Soc. Am. A 24(9) 2921-2931 (2007)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (13)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription