Abstract

Fluorescence enhancement from a multilayered substrate fabricated with Ag and Al2O3 was investigated using fluorescein, rhodamine B, Cy3, and Cy5 as fluorophores. The change in the fluorescence enhancement with Al2O3 had two peaks and one valley in the range from 0 to 300nm of Al2O3 thickness, and such peaks and valley were found to appear periodically. Moreover, the reflection of the excitation light from the multilayered substrate was investigated. The reflection of the excitation light periodically changed depending on the Al2O3 thickness as well, and the maximum reflection was observed near the Al2O3 thickness of the peak fluorescence enhancement. It was found that the periodic changes of the fluorescence enhancement and the reflection of the excitation light could be explained, for the most part, with the integral multiples of the λ/4 derived by a simple interference theory.

© 2010 Optical Society of America

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2008

2007

C. R. Sabanayagam and J. R. Lakowicz, “Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles,” Nucleic Acid Res. 35, e13 (2007).

E. G. Matveeva, I. Gryczynski, A. Barnett, Z. Leonenko, J. R. Lakowicz, and Z. Gryczynski, “Metal particle-enhanced fluorescent immunoassay on metal mirrors,” Anal. Biochem. 363, 239-245 (2007).
[CrossRef]

E. Le Moal, E. Fort, S. Lévêsque-Fort, F. P. Cordelières, M.-P. Fontaine-Aupart, and C. Ricolleau, “Enhanced fluorescence cell imaging with metal-coated slides,” Biophys. J. 92, 2150-2169 (2007).
[CrossRef]

E. Le Moal, E. Fort, S. Lévêsque-Fort, A. Janin, H. Murata, F. P. Cordelières, M. P. Fontaine-Aupart, and C. Ricolleau, “Mirror slides for high-sensitivity cell and tissue fluorescence imaging,” J. Biomed. Opt. 12, 024030 (2007).

2006

Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence image and time-resolved studies using single-molecule spectroscopy,” Anal. Chem. 78, 6238-6245 (2006).
[CrossRef]

Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence image and time-resolved studies using single-molecule spectroscopy,” Anal. Chem. 78, 6238-6245 (2006).
[CrossRef]

2005

H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005).
[CrossRef]

2004

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

2003

J. Malicka, I. Gryczynski, Z. Gryczynski, and J. R. Lakowicz, “Effect of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides,” Anal. Biochem. 315, 57-66 (2003).
[CrossRef]

2002

J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski, “Radiative decay engineering: 2. Effect of silver island films on fluorescence intensity, lifetime, and resonance energy transfer,” Anal. Biochem. 301, 261-277 (2002).
[CrossRef]

2001

J. R. Lakowicz, “Radiative decay engineering: biophysical and biomedical applications,” Anal. Biochem. 298, 1-24 (2001).
[CrossRef]

1999

1997

1994

1985

Akimoto, T.

Aroca, R.

Aslan, K.

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

Badugu, R.

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

Barnett, A.

E. G. Matveeva, I. Gryczynski, A. Barnett, Z. Leonenko, J. R. Lakowicz, and Z. Gryczynski, “Metal particle-enhanced fluorescent immunoassay on metal mirrors,” Anal. Biochem. 363, 239-245 (2007).
[CrossRef]

Cerovic, G.

H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005).
[CrossRef]

Chardon, A.

H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005).
[CrossRef]

Choumane, H.

H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005).
[CrossRef]

Cordelières, F. P.

E. Le Moal, E. Fort, S. Lévêsque-Fort, A. Janin, H. Murata, F. P. Cordelières, M. P. Fontaine-Aupart, and C. Ricolleau, “Mirror slides for high-sensitivity cell and tissue fluorescence imaging,” J. Biomed. Opt. 12, 024030 (2007).

E. Le Moal, E. Fort, S. Lévêsque-Fort, F. P. Cordelières, M.-P. Fontaine-Aupart, and C. Ricolleau, “Enhanced fluorescence cell imaging with metal-coated slides,” Biophys. J. 92, 2150-2169 (2007).
[CrossRef]

D'Auria, S.

J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski, “Radiative decay engineering: 2. Effect of silver island films on fluorescence intensity, lifetime, and resonance energy transfer,” Anal. Biochem. 301, 261-277 (2002).
[CrossRef]

Desaja-Gonzalez, J.

Fang, J.

J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski, “Radiative decay engineering: 2. Effect of silver island films on fluorescence intensity, lifetime, and resonance energy transfer,” Anal. Biochem. 301, 261-277 (2002).
[CrossRef]

Fontaine-Aupart, M. P.

E. Le Moal, E. Fort, S. Lévêsque-Fort, A. Janin, H. Murata, F. P. Cordelières, M. P. Fontaine-Aupart, and C. Ricolleau, “Mirror slides for high-sensitivity cell and tissue fluorescence imaging,” J. Biomed. Opt. 12, 024030 (2007).

Fontaine-Aupart, M.-P.

E. Le Moal, E. Fort, S. Lévêsque-Fort, F. P. Cordelières, M.-P. Fontaine-Aupart, and C. Ricolleau, “Enhanced fluorescence cell imaging with metal-coated slides,” Biophys. J. 92, 2150-2169 (2007).
[CrossRef]

Fort, E.

E. Le Moal, E. Fort, S. Lévêsque-Fort, F. P. Cordelières, M.-P. Fontaine-Aupart, and C. Ricolleau, “Enhanced fluorescence cell imaging with metal-coated slides,” Biophys. J. 92, 2150-2169 (2007).
[CrossRef]

E. Le Moal, E. Fort, S. Lévêsque-Fort, A. Janin, H. Murata, F. P. Cordelières, M. P. Fontaine-Aupart, and C. Ricolleau, “Mirror slides for high-sensitivity cell and tissue fluorescence imaging,” J. Biomed. Opt. 12, 024030 (2007).

Fu, Y.

Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence image and time-resolved studies using single-molecule spectroscopy,” Anal. Chem. 78, 6238-6245 (2006).
[CrossRef]

Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence image and time-resolved studies using single-molecule spectroscopy,” Anal. Chem. 78, 6238-6245 (2006).
[CrossRef]

Geddes, C. D.

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

Goutel, C.

H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005).
[CrossRef]

Gryczynski, I.

E. G. Matveeva, I. Gryczynski, A. Barnett, Z. Leonenko, J. R. Lakowicz, and Z. Gryczynski, “Metal particle-enhanced fluorescent immunoassay on metal mirrors,” Anal. Biochem. 363, 239-245 (2007).
[CrossRef]

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

J. Malicka, I. Gryczynski, Z. Gryczynski, and J. R. Lakowicz, “Effect of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides,” Anal. Biochem. 315, 57-66 (2003).
[CrossRef]

J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski, “Radiative decay engineering: 2. Effect of silver island films on fluorescence intensity, lifetime, and resonance energy transfer,” Anal. Biochem. 301, 261-277 (2002).
[CrossRef]

Gryczynski, Z.

E. G. Matveeva, I. Gryczynski, A. Barnett, Z. Leonenko, J. R. Lakowicz, and Z. Gryczynski, “Metal particle-enhanced fluorescent immunoassay on metal mirrors,” Anal. Biochem. 363, 239-245 (2007).
[CrossRef]

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

J. Malicka, I. Gryczynski, Z. Gryczynski, and J. R. Lakowicz, “Effect of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides,” Anal. Biochem. 315, 57-66 (2003).
[CrossRef]

J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski, “Radiative decay engineering: 2. Effect of silver island films on fluorescence intensity, lifetime, and resonance energy transfer,” Anal. Biochem. 301, 261-277 (2002).
[CrossRef]

Ha, N.

H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005).
[CrossRef]

Hall, D. G.

Holland, W. R.

Huang, J.

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

Janin, A.

E. Le Moal, E. Fort, S. Lévêsque-Fort, A. Janin, H. Murata, F. P. Cordelières, M. P. Fontaine-Aupart, and C. Ricolleau, “Mirror slides for high-sensitivity cell and tissue fluorescence imaging,” J. Biomed. Opt. 12, 024030 (2007).

Karube, I.

King, O.

Lakowicz, J. R.

E. G. Matveeva, I. Gryczynski, A. Barnett, Z. Leonenko, J. R. Lakowicz, and Z. Gryczynski, “Metal particle-enhanced fluorescent immunoassay on metal mirrors,” Anal. Biochem. 363, 239-245 (2007).
[CrossRef]

C. R. Sabanayagam and J. R. Lakowicz, “Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles,” Nucleic Acid Res. 35, e13 (2007).

Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence image and time-resolved studies using single-molecule spectroscopy,” Anal. Chem. 78, 6238-6245 (2006).
[CrossRef]

Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence image and time-resolved studies using single-molecule spectroscopy,” Anal. Chem. 78, 6238-6245 (2006).
[CrossRef]

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

J. Malicka, I. Gryczynski, Z. Gryczynski, and J. R. Lakowicz, “Effect of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides,” Anal. Biochem. 315, 57-66 (2003).
[CrossRef]

J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski, “Radiative decay engineering: 2. Effect of silver island films on fluorescence intensity, lifetime, and resonance energy transfer,” Anal. Biochem. 301, 261-277 (2002).
[CrossRef]

J. R. Lakowicz, “Radiative decay engineering: biophysical and biomedical applications,” Anal. Biochem. 298, 1-24 (2001).
[CrossRef]

Le Moal, E.

E. Le Moal, E. Fort, S. Lévêsque-Fort, F. P. Cordelières, M.-P. Fontaine-Aupart, and C. Ricolleau, “Enhanced fluorescence cell imaging with metal-coated slides,” Biophys. J. 92, 2150-2169 (2007).
[CrossRef]

E. Le Moal, E. Fort, S. Lévêsque-Fort, A. Janin, H. Murata, F. P. Cordelières, M. P. Fontaine-Aupart, and C. Ricolleau, “Mirror slides for high-sensitivity cell and tissue fluorescence imaging,” J. Biomed. Opt. 12, 024030 (2007).

Leonenko, Z.

E. G. Matveeva, I. Gryczynski, A. Barnett, Z. Leonenko, J. R. Lakowicz, and Z. Gryczynski, “Metal particle-enhanced fluorescent immunoassay on metal mirrors,” Anal. Biochem. 363, 239-245 (2007).
[CrossRef]

Lévêsque-Fort, S.

E. Le Moal, E. Fort, S. Lévêsque-Fort, A. Janin, H. Murata, F. P. Cordelières, M. P. Fontaine-Aupart, and C. Ricolleau, “Mirror slides for high-sensitivity cell and tissue fluorescence imaging,” J. Biomed. Opt. 12, 024030 (2007).

E. Le Moal, E. Fort, S. Lévêsque-Fort, F. P. Cordelières, M.-P. Fontaine-Aupart, and C. Ricolleau, “Enhanced fluorescence cell imaging with metal-coated slides,” Biophys. J. 92, 2150-2169 (2007).
[CrossRef]

Lukomska, J.

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

Malicka, J.

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

J. Malicka, I. Gryczynski, Z. Gryczynski, and J. R. Lakowicz, “Effect of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides,” Anal. Biochem. 315, 57-66 (2003).
[CrossRef]

J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski, “Radiative decay engineering: 2. Effect of silver island films on fluorescence intensity, lifetime, and resonance energy transfer,” Anal. Biochem. 301, 261-277 (2002).
[CrossRef]

Matveeva, E.

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

Matveeva, E. G.

E. G. Matveeva, I. Gryczynski, A. Barnett, Z. Leonenko, J. R. Lakowicz, and Z. Gryczynski, “Metal particle-enhanced fluorescent immunoassay on metal mirrors,” Anal. Biochem. 363, 239-245 (2007).
[CrossRef]

Murata, H.

E. Le Moal, E. Fort, S. Lévêsque-Fort, A. Janin, H. Murata, F. P. Cordelières, M. P. Fontaine-Aupart, and C. Ricolleau, “Mirror slides for high-sensitivity cell and tissue fluorescence imaging,” J. Biomed. Opt. 12, 024030 (2007).

Nelep, C.

H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005).
[CrossRef]

Reymond, G. O.

H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005).
[CrossRef]

Ricolleau, C.

E. Le Moal, E. Fort, S. Lévêsque-Fort, A. Janin, H. Murata, F. P. Cordelières, M. P. Fontaine-Aupart, and C. Ricolleau, “Mirror slides for high-sensitivity cell and tissue fluorescence imaging,” J. Biomed. Opt. 12, 024030 (2007).

E. Le Moal, E. Fort, S. Lévêsque-Fort, F. P. Cordelières, M.-P. Fontaine-Aupart, and C. Ricolleau, “Enhanced fluorescence cell imaging with metal-coated slides,” Biophys. J. 92, 2150-2169 (2007).
[CrossRef]

Rodriguez-Llorente, S.

Sabanayagam, C. R.

C. R. Sabanayagam and J. R. Lakowicz, “Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles,” Nucleic Acid Res. 35, e13 (2007).

Shen, Y.

J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski, “Radiative decay engineering: 2. Effect of silver island films on fluorescence intensity, lifetime, and resonance energy transfer,” Anal. Biochem. 301, 261-277 (2002).
[CrossRef]

Sigg, C.

Sullivan, K. G.

Tarcha, P. J.

Vallet, F.

H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005).
[CrossRef]

Weisbuch, C.

H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005).
[CrossRef]

Yasuda, M.

Zhang, J.

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

Anal. Biochem.

J. R. Lakowicz, “Radiative decay engineering: biophysical and biomedical applications,” Anal. Biochem. 298, 1-24 (2001).
[CrossRef]

J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski, “Radiative decay engineering: 2. Effect of silver island films on fluorescence intensity, lifetime, and resonance energy transfer,” Anal. Biochem. 301, 261-277 (2002).
[CrossRef]

J. Malicka, I. Gryczynski, Z. Gryczynski, and J. R. Lakowicz, “Effect of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides,” Anal. Biochem. 315, 57-66 (2003).
[CrossRef]

E. G. Matveeva, I. Gryczynski, A. Barnett, Z. Leonenko, J. R. Lakowicz, and Z. Gryczynski, “Metal particle-enhanced fluorescent immunoassay on metal mirrors,” Anal. Biochem. 363, 239-245 (2007).
[CrossRef]

Anal. Chem.

Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence image and time-resolved studies using single-molecule spectroscopy,” Anal. Chem. 78, 6238-6245 (2006).
[CrossRef]

Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence image and time-resolved studies using single-molecule spectroscopy,” Anal. Chem. 78, 6238-6245 (2006).
[CrossRef]

Appl. Opt.

Appl. Phys. Lett.

H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005).
[CrossRef]

Appl. Spectrosc.

Biophys. J.

E. Le Moal, E. Fort, S. Lévêsque-Fort, F. P. Cordelières, M.-P. Fontaine-Aupart, and C. Ricolleau, “Enhanced fluorescence cell imaging with metal-coated slides,” Biophys. J. 92, 2150-2169 (2007).
[CrossRef]

J. Biomed. Opt.

E. Le Moal, E. Fort, S. Lévêsque-Fort, A. Janin, H. Murata, F. P. Cordelières, M. P. Fontaine-Aupart, and C. Ricolleau, “Mirror slides for high-sensitivity cell and tissue fluorescence imaging,” J. Biomed. Opt. 12, 024030 (2007).

J. Fluoresc.

J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004).
[CrossRef]

J. Opt. Soc. Am. B

Nucleic Acid Res.

C. R. Sabanayagam and J. R. Lakowicz, “Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles,” Nucleic Acid Res. 35, e13 (2007).

Opt. Lett.

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Figures (4)

Fig. 1
Fig. 1

(a) Schema of the multilayered substrate and Al 2 O 3 substrate. (b) Experimental setup.

Fig. 2
Fig. 2

Fluorescence enhancement as function of Al 2 O 3 thickness for (a) fluorescein and Cy5 and (b) Rhodamine B and Cy3.

Fig. 3
Fig. 3

Enhancement of the relative reflection of the excitation light as function of Al 2 O 3 thickness.

Fig. 4
Fig. 4

Schema of the multilayered substrate for the interference calculation.

Tables (1)

Tables Icon

Table 1 Al 2 O 3 Thickness Providing Peaks, Valley, and Enhancement Ratio for the Fluorescence Enhancement and Relative Reflection of Excitation Light a

Equations (2)

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n 2 d cos θ 2 = ( 2 m + 1 ) λ 4 ,
n 2 d cos θ 2 = ( 2 m ) λ 4 , m = 0 , 1 , 2 ,

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