Abstract

We present a differential interference contrast microscope using photonic crystals capable of real-time capture of both phase and amplitude components independently without moving parts. Unlike previous methods using rotating polarizers to discriminate each component, we propose using a special camera equipped with an arrayed polarizer whose instant polarization measurement allows real-time acquisition of the phase gradient information. A two-image algorithm is used to reconstruct the phase two- dimensional distribution of biological samples from the gradient information with a transmission-type microscope. We also talk about deducing a sample’s three-dimensional shape for a reflection-type microscope. The efficiency of the method is demonstrated experimentally.

© 2009 Optical Society of America

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