Abstract

Noninvasive imaging techniques such as optical coherence tomography (OCT) are being widely used for early diagnostics of a variety of pathologies. Traditional tissue preparation for histological evaluation alters the dimensions of histological images such that differences between the dimensions of histologic and OCT images can be as great as 2.5-fold. This discrepancy complicates quantitative interpretation of OCT images. Here we used a steel ring sewn to the pigskin to avoid tissue deformation due to excision and fixation and used a surgical suture as a fixed tissue marker. With these techniques, we achieved good correlation between OCT and histological images with differences of less than 10%. The resulting method significantly enhances the diagnostic capabilities of the OCT technique.

© 2007 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
    [CrossRef] [PubMed]
  2. M. E. Brezinski and J. G. Fujimoto, "Optical coherence tomography: high-resolution imaging in nontransparent tissue," IEEE J. Sel. Top. Quantum Electron. 5, 1185-1192 (1999).
    [CrossRef]
  3. J. M. Schmitt, "Optical coherence tomography (OCT): a review," IEEE J. Sel. Top. Quantum Electron. 5, 1205-1215 (1999).
    [CrossRef]
  4. M. Gloesmann, B. Hermann, C. Schubert, H. Sattmann, P. K. Ahnelt, and W. Drexler, "Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography," Invest. Ophthalmol. Visual Sci. 44, 1696-1702 (2003).
    [CrossRef]
  5. S. A. Boppart, M. E. Brezinski, C. Pitris, and J. G. Fujimoto, "Optical coherence tomography for neurosurgical imaging of human intracortical melanoma," Neurosurgery 43, 834-841 (1998).
    [CrossRef] [PubMed]
  6. F. G. Bechara, T. Gambichler, M. Stucker, A. Orlikov, S. Rotterdam, P. Altmeyer, and K. Hoffmann, "Histomorphologic correlation with routine histology and optical coherence tomography," Skin Res. Technol. 10, 169-173 (2004).
    [CrossRef] [PubMed]
  7. N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
    [CrossRef]
  8. A. I. Kholodnykh, I. Y. Petrova, K. V. Larin, M. Motamedi, and R. O. Esenaliev, "Precision of measurement of tissue optical properties with optical coherence tomography," Appl. Opt. 42, 3027-3037 (2003).
    [CrossRef] [PubMed]
  9. R. O. Esenaliev, K. V. Larin, I. V. Larina, and M. Motamedi, "Noninvasive monitoring of glucose concentration with optical coherence tomography," Opt. Lett. 26, 992-994 (2001).
    [CrossRef]
  10. D. Hopwood, "Fixation and fixatives," in Theory and Practice of Histological Techniques, J.D.Bancroft and M.Gamble, eds. (Churchill Livingstone, 2002), pp. 71-72.
  11. V. V. Sapozhnikova, N. M. Shakhova, V. A. Kamensky, S. A. Petrova, L. B. Snopova, and R. V. Kuranov, "Capabilities of fluorescence spectroscopy using 5-ALA and optical coherence tomography for diagnosis of neoplastic processes in the uterine cervix and vulva," Laser Phys. 15, 1664-1673 (2005).
  12. P. Nghiem, P. H. Mckee, and H. A. Haynes, "Merkel cell (cutaneous neuroendocrine) carcinoma," in Atlas of Clinical Oncology: Skin Cancer, A.J.Sober and F.H.Haluska, eds. (BC Decker Inc., 2001), pp. 132-133.
  13. N. M. Shakhova, V. M. Gelikonov, V. A. Kamensky, R. V. Kuranov, and I. V. Turchin, "Clinical aspects of the endoscopic optical coherence tomography and the ways for improving its diagnostic value," Laser Phys. 12, 617-626 (2002).
  14. A. M. Sergeev, V. M. Gelikonov, G. V. Gelikonov, F. I. Feldchtein, R. V. Kuranov, N. D. Gladkova, N. M. Shakhova, L. B. Snopova, A. V. Shakhov, I. A. Kuznetzova, A. N. Denisenko, V. V. Pochinko, Yu. P. Chumakov, and O. S. Streltzova, "In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa," Opt. Express 1, 432-440 (1997).
    [CrossRef] [PubMed]

2005 (1)

V. V. Sapozhnikova, N. M. Shakhova, V. A. Kamensky, S. A. Petrova, L. B. Snopova, and R. V. Kuranov, "Capabilities of fluorescence spectroscopy using 5-ALA and optical coherence tomography for diagnosis of neoplastic processes in the uterine cervix and vulva," Laser Phys. 15, 1664-1673 (2005).

2004 (1)

F. G. Bechara, T. Gambichler, M. Stucker, A. Orlikov, S. Rotterdam, P. Altmeyer, and K. Hoffmann, "Histomorphologic correlation with routine histology and optical coherence tomography," Skin Res. Technol. 10, 169-173 (2004).
[CrossRef] [PubMed]

2003 (2)

A. I. Kholodnykh, I. Y. Petrova, K. V. Larin, M. Motamedi, and R. O. Esenaliev, "Precision of measurement of tissue optical properties with optical coherence tomography," Appl. Opt. 42, 3027-3037 (2003).
[CrossRef] [PubMed]

M. Gloesmann, B. Hermann, C. Schubert, H. Sattmann, P. K. Ahnelt, and W. Drexler, "Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography," Invest. Ophthalmol. Visual Sci. 44, 1696-1702 (2003).
[CrossRef]

2002 (1)

N. M. Shakhova, V. M. Gelikonov, V. A. Kamensky, R. V. Kuranov, and I. V. Turchin, "Clinical aspects of the endoscopic optical coherence tomography and the ways for improving its diagnostic value," Laser Phys. 12, 617-626 (2002).

2001 (1)

2000 (1)

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

1999 (2)

M. E. Brezinski and J. G. Fujimoto, "Optical coherence tomography: high-resolution imaging in nontransparent tissue," IEEE J. Sel. Top. Quantum Electron. 5, 1185-1192 (1999).
[CrossRef]

J. M. Schmitt, "Optical coherence tomography (OCT): a review," IEEE J. Sel. Top. Quantum Electron. 5, 1205-1215 (1999).
[CrossRef]

1998 (1)

S. A. Boppart, M. E. Brezinski, C. Pitris, and J. G. Fujimoto, "Optical coherence tomography for neurosurgical imaging of human intracortical melanoma," Neurosurgery 43, 834-841 (1998).
[CrossRef] [PubMed]

1997 (1)

1991 (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Ahnelt, P. K.

M. Gloesmann, B. Hermann, C. Schubert, H. Sattmann, P. K. Ahnelt, and W. Drexler, "Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography," Invest. Ophthalmol. Visual Sci. 44, 1696-1702 (2003).
[CrossRef]

Altmeyer, P.

F. G. Bechara, T. Gambichler, M. Stucker, A. Orlikov, S. Rotterdam, P. Altmeyer, and K. Hoffmann, "Histomorphologic correlation with routine histology and optical coherence tomography," Skin Res. Technol. 10, 169-173 (2004).
[CrossRef] [PubMed]

Bechara, F. G.

F. G. Bechara, T. Gambichler, M. Stucker, A. Orlikov, S. Rotterdam, P. Altmeyer, and K. Hoffmann, "Histomorphologic correlation with routine histology and optical coherence tomography," Skin Res. Technol. 10, 169-173 (2004).
[CrossRef] [PubMed]

Boppart, S. A.

S. A. Boppart, M. E. Brezinski, C. Pitris, and J. G. Fujimoto, "Optical coherence tomography for neurosurgical imaging of human intracortical melanoma," Neurosurgery 43, 834-841 (1998).
[CrossRef] [PubMed]

Brezinski, M. E.

M. E. Brezinski and J. G. Fujimoto, "Optical coherence tomography: high-resolution imaging in nontransparent tissue," IEEE J. Sel. Top. Quantum Electron. 5, 1185-1192 (1999).
[CrossRef]

S. A. Boppart, M. E. Brezinski, C. Pitris, and J. G. Fujimoto, "Optical coherence tomography for neurosurgical imaging of human intracortical melanoma," Neurosurgery 43, 834-841 (1998).
[CrossRef] [PubMed]

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Chumakov, Y. P.

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

Chumakov, Yu. P.

Denisenko, A. N.

Drexler, W.

M. Gloesmann, B. Hermann, C. Schubert, H. Sattmann, P. K. Ahnelt, and W. Drexler, "Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography," Invest. Ophthalmol. Visual Sci. 44, 1696-1702 (2003).
[CrossRef]

Esenaliev, R. O.

Feldchtein, F. I.

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

A. M. Sergeev, V. M. Gelikonov, G. V. Gelikonov, F. I. Feldchtein, R. V. Kuranov, N. D. Gladkova, N. M. Shakhova, L. B. Snopova, A. V. Shakhov, I. A. Kuznetzova, A. N. Denisenko, V. V. Pochinko, Yu. P. Chumakov, and O. S. Streltzova, "In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa," Opt. Express 1, 432-440 (1997).
[CrossRef] [PubMed]

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Fujimoto, J. G.

M. E. Brezinski and J. G. Fujimoto, "Optical coherence tomography: high-resolution imaging in nontransparent tissue," IEEE J. Sel. Top. Quantum Electron. 5, 1185-1192 (1999).
[CrossRef]

S. A. Boppart, M. E. Brezinski, C. Pitris, and J. G. Fujimoto, "Optical coherence tomography for neurosurgical imaging of human intracortical melanoma," Neurosurgery 43, 834-841 (1998).
[CrossRef] [PubMed]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Gambichler, T.

F. G. Bechara, T. Gambichler, M. Stucker, A. Orlikov, S. Rotterdam, P. Altmeyer, and K. Hoffmann, "Histomorphologic correlation with routine histology and optical coherence tomography," Skin Res. Technol. 10, 169-173 (2004).
[CrossRef] [PubMed]

Gelikonov, G. V.

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

A. M. Sergeev, V. M. Gelikonov, G. V. Gelikonov, F. I. Feldchtein, R. V. Kuranov, N. D. Gladkova, N. M. Shakhova, L. B. Snopova, A. V. Shakhov, I. A. Kuznetzova, A. N. Denisenko, V. V. Pochinko, Yu. P. Chumakov, and O. S. Streltzova, "In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa," Opt. Express 1, 432-440 (1997).
[CrossRef] [PubMed]

Gelikonov, V. M.

N. M. Shakhova, V. M. Gelikonov, V. A. Kamensky, R. V. Kuranov, and I. V. Turchin, "Clinical aspects of the endoscopic optical coherence tomography and the ways for improving its diagnostic value," Laser Phys. 12, 617-626 (2002).

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

A. M. Sergeev, V. M. Gelikonov, G. V. Gelikonov, F. I. Feldchtein, R. V. Kuranov, N. D. Gladkova, N. M. Shakhova, L. B. Snopova, A. V. Shakhov, I. A. Kuznetzova, A. N. Denisenko, V. V. Pochinko, Yu. P. Chumakov, and O. S. Streltzova, "In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa," Opt. Express 1, 432-440 (1997).
[CrossRef] [PubMed]

Gladkova, N. D.

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

A. M. Sergeev, V. M. Gelikonov, G. V. Gelikonov, F. I. Feldchtein, R. V. Kuranov, N. D. Gladkova, N. M. Shakhova, L. B. Snopova, A. V. Shakhov, I. A. Kuznetzova, A. N. Denisenko, V. V. Pochinko, Yu. P. Chumakov, and O. S. Streltzova, "In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa," Opt. Express 1, 432-440 (1997).
[CrossRef] [PubMed]

Gloesmann, M.

M. Gloesmann, B. Hermann, C. Schubert, H. Sattmann, P. K. Ahnelt, and W. Drexler, "Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography," Invest. Ophthalmol. Visual Sci. 44, 1696-1702 (2003).
[CrossRef]

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Haynes, H. A.

P. Nghiem, P. H. Mckee, and H. A. Haynes, "Merkel cell (cutaneous neuroendocrine) carcinoma," in Atlas of Clinical Oncology: Skin Cancer, A.J.Sober and F.H.Haluska, eds. (BC Decker Inc., 2001), pp. 132-133.

Hee, M. R.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Hermann, B.

M. Gloesmann, B. Hermann, C. Schubert, H. Sattmann, P. K. Ahnelt, and W. Drexler, "Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography," Invest. Ophthalmol. Visual Sci. 44, 1696-1702 (2003).
[CrossRef]

Hoffmann, K.

F. G. Bechara, T. Gambichler, M. Stucker, A. Orlikov, S. Rotterdam, P. Altmeyer, and K. Hoffmann, "Histomorphologic correlation with routine histology and optical coherence tomography," Skin Res. Technol. 10, 169-173 (2004).
[CrossRef] [PubMed]

Hopwood, D.

D. Hopwood, "Fixation and fixatives," in Theory and Practice of Histological Techniques, J.D.Bancroft and M.Gamble, eds. (Churchill Livingstone, 2002), pp. 71-72.

Huang, D.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Kamensky, V. A.

V. V. Sapozhnikova, N. M. Shakhova, V. A. Kamensky, S. A. Petrova, L. B. Snopova, and R. V. Kuranov, "Capabilities of fluorescence spectroscopy using 5-ALA and optical coherence tomography for diagnosis of neoplastic processes in the uterine cervix and vulva," Laser Phys. 15, 1664-1673 (2005).

N. M. Shakhova, V. M. Gelikonov, V. A. Kamensky, R. V. Kuranov, and I. V. Turchin, "Clinical aspects of the endoscopic optical coherence tomography and the ways for improving its diagnostic value," Laser Phys. 12, 617-626 (2002).

Kholodnykh, A. I.

Kuranov, R. V.

V. V. Sapozhnikova, N. M. Shakhova, V. A. Kamensky, S. A. Petrova, L. B. Snopova, and R. V. Kuranov, "Capabilities of fluorescence spectroscopy using 5-ALA and optical coherence tomography for diagnosis of neoplastic processes in the uterine cervix and vulva," Laser Phys. 15, 1664-1673 (2005).

N. M. Shakhova, V. M. Gelikonov, V. A. Kamensky, R. V. Kuranov, and I. V. Turchin, "Clinical aspects of the endoscopic optical coherence tomography and the ways for improving its diagnostic value," Laser Phys. 12, 617-626 (2002).

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

A. M. Sergeev, V. M. Gelikonov, G. V. Gelikonov, F. I. Feldchtein, R. V. Kuranov, N. D. Gladkova, N. M. Shakhova, L. B. Snopova, A. V. Shakhov, I. A. Kuznetzova, A. N. Denisenko, V. V. Pochinko, Yu. P. Chumakov, and O. S. Streltzova, "In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa," Opt. Express 1, 432-440 (1997).
[CrossRef] [PubMed]

Kuznetzova, I. A.

Larin, K. V.

Larina, I. V.

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Mckee, P. H.

P. Nghiem, P. H. Mckee, and H. A. Haynes, "Merkel cell (cutaneous neuroendocrine) carcinoma," in Atlas of Clinical Oncology: Skin Cancer, A.J.Sober and F.H.Haluska, eds. (BC Decker Inc., 2001), pp. 132-133.

Motamedi, M.

Nasonova, V. A.

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

Nghiem, P.

P. Nghiem, P. H. Mckee, and H. A. Haynes, "Merkel cell (cutaneous neuroendocrine) carcinoma," in Atlas of Clinical Oncology: Skin Cancer, A.J.Sober and F.H.Haluska, eds. (BC Decker Inc., 2001), pp. 132-133.

Nikulin, N. K.

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

Orlikov, A.

F. G. Bechara, T. Gambichler, M. Stucker, A. Orlikov, S. Rotterdam, P. Altmeyer, and K. Hoffmann, "Histomorphologic correlation with routine histology and optical coherence tomography," Skin Res. Technol. 10, 169-173 (2004).
[CrossRef] [PubMed]

Petrova, G. A.

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

Petrova, I. Y.

Petrova, S. A.

V. V. Sapozhnikova, N. M. Shakhova, V. A. Kamensky, S. A. Petrova, L. B. Snopova, and R. V. Kuranov, "Capabilities of fluorescence spectroscopy using 5-ALA and optical coherence tomography for diagnosis of neoplastic processes in the uterine cervix and vulva," Laser Phys. 15, 1664-1673 (2005).

Pitris, C.

S. A. Boppart, M. E. Brezinski, C. Pitris, and J. G. Fujimoto, "Optical coherence tomography for neurosurgical imaging of human intracortical melanoma," Neurosurgery 43, 834-841 (1998).
[CrossRef] [PubMed]

Pochinko, V. V.

Puliafito, C. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Radenska-Lopovok, S. G.

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

Rotterdam, S.

F. G. Bechara, T. Gambichler, M. Stucker, A. Orlikov, S. Rotterdam, P. Altmeyer, and K. Hoffmann, "Histomorphologic correlation with routine histology and optical coherence tomography," Skin Res. Technol. 10, 169-173 (2004).
[CrossRef] [PubMed]

Sapozhnikova, V. V.

V. V. Sapozhnikova, N. M. Shakhova, V. A. Kamensky, S. A. Petrova, L. B. Snopova, and R. V. Kuranov, "Capabilities of fluorescence spectroscopy using 5-ALA and optical coherence tomography for diagnosis of neoplastic processes in the uterine cervix and vulva," Laser Phys. 15, 1664-1673 (2005).

Sattmann, H.

M. Gloesmann, B. Hermann, C. Schubert, H. Sattmann, P. K. Ahnelt, and W. Drexler, "Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography," Invest. Ophthalmol. Visual Sci. 44, 1696-1702 (2003).
[CrossRef]

Schmitt, J. M.

J. M. Schmitt, "Optical coherence tomography (OCT): a review," IEEE J. Sel. Top. Quantum Electron. 5, 1205-1215 (1999).
[CrossRef]

Schubert, C.

M. Gloesmann, B. Hermann, C. Schubert, H. Sattmann, P. K. Ahnelt, and W. Drexler, "Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography," Invest. Ophthalmol. Visual Sci. 44, 1696-1702 (2003).
[CrossRef]

Schuman, J. S.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Sergeev, A. M.

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

A. M. Sergeev, V. M. Gelikonov, G. V. Gelikonov, F. I. Feldchtein, R. V. Kuranov, N. D. Gladkova, N. M. Shakhova, L. B. Snopova, A. V. Shakhov, I. A. Kuznetzova, A. N. Denisenko, V. V. Pochinko, Yu. P. Chumakov, and O. S. Streltzova, "In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa," Opt. Express 1, 432-440 (1997).
[CrossRef] [PubMed]

Shakhov, A. V.

Shakhova, N. M.

V. V. Sapozhnikova, N. M. Shakhova, V. A. Kamensky, S. A. Petrova, L. B. Snopova, and R. V. Kuranov, "Capabilities of fluorescence spectroscopy using 5-ALA and optical coherence tomography for diagnosis of neoplastic processes in the uterine cervix and vulva," Laser Phys. 15, 1664-1673 (2005).

N. M. Shakhova, V. M. Gelikonov, V. A. Kamensky, R. V. Kuranov, and I. V. Turchin, "Clinical aspects of the endoscopic optical coherence tomography and the ways for improving its diagnostic value," Laser Phys. 12, 617-626 (2002).

A. M. Sergeev, V. M. Gelikonov, G. V. Gelikonov, F. I. Feldchtein, R. V. Kuranov, N. D. Gladkova, N. M. Shakhova, L. B. Snopova, A. V. Shakhov, I. A. Kuznetzova, A. N. Denisenko, V. V. Pochinko, Yu. P. Chumakov, and O. S. Streltzova, "In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa," Opt. Express 1, 432-440 (1997).
[CrossRef] [PubMed]

Snopova, L. B.

V. V. Sapozhnikova, N. M. Shakhova, V. A. Kamensky, S. A. Petrova, L. B. Snopova, and R. V. Kuranov, "Capabilities of fluorescence spectroscopy using 5-ALA and optical coherence tomography for diagnosis of neoplastic processes in the uterine cervix and vulva," Laser Phys. 15, 1664-1673 (2005).

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

A. M. Sergeev, V. M. Gelikonov, G. V. Gelikonov, F. I. Feldchtein, R. V. Kuranov, N. D. Gladkova, N. M. Shakhova, L. B. Snopova, A. V. Shakhov, I. A. Kuznetzova, A. N. Denisenko, V. V. Pochinko, Yu. P. Chumakov, and O. S. Streltzova, "In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa," Opt. Express 1, 432-440 (1997).
[CrossRef] [PubMed]

Stinson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Streltzova, O. S.

Stucker, M.

F. G. Bechara, T. Gambichler, M. Stucker, A. Orlikov, S. Rotterdam, P. Altmeyer, and K. Hoffmann, "Histomorphologic correlation with routine histology and optical coherence tomography," Skin Res. Technol. 10, 169-173 (2004).
[CrossRef] [PubMed]

Swanson, E. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Turchin, I. V.

N. M. Shakhova, V. M. Gelikonov, V. A. Kamensky, R. V. Kuranov, and I. V. Turchin, "Clinical aspects of the endoscopic optical coherence tomography and the ways for improving its diagnostic value," Laser Phys. 12, 617-626 (2002).

Appl. Opt. (1)

IEEE J. Sel. Top. Quantum Electron. (2)

M. E. Brezinski and J. G. Fujimoto, "Optical coherence tomography: high-resolution imaging in nontransparent tissue," IEEE J. Sel. Top. Quantum Electron. 5, 1185-1192 (1999).
[CrossRef]

J. M. Schmitt, "Optical coherence tomography (OCT): a review," IEEE J. Sel. Top. Quantum Electron. 5, 1205-1215 (1999).
[CrossRef]

Invest. Ophthalmol. Visual Sci. (1)

M. Gloesmann, B. Hermann, C. Schubert, H. Sattmann, P. K. Ahnelt, and W. Drexler, "Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography," Invest. Ophthalmol. Visual Sci. 44, 1696-1702 (2003).
[CrossRef]

Laser Phys. (2)

V. V. Sapozhnikova, N. M. Shakhova, V. A. Kamensky, S. A. Petrova, L. B. Snopova, and R. V. Kuranov, "Capabilities of fluorescence spectroscopy using 5-ALA and optical coherence tomography for diagnosis of neoplastic processes in the uterine cervix and vulva," Laser Phys. 15, 1664-1673 (2005).

N. M. Shakhova, V. M. Gelikonov, V. A. Kamensky, R. V. Kuranov, and I. V. Turchin, "Clinical aspects of the endoscopic optical coherence tomography and the ways for improving its diagnostic value," Laser Phys. 12, 617-626 (2002).

Neurosurgery (1)

S. A. Boppart, M. E. Brezinski, C. Pitris, and J. G. Fujimoto, "Optical coherence tomography for neurosurgical imaging of human intracortical melanoma," Neurosurgery 43, 834-841 (1998).
[CrossRef] [PubMed]

Opt. Express (1)

Opt. Lett. (1)

Science (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991).
[CrossRef] [PubMed]

Skin Res. Technol. (2)

F. G. Bechara, T. Gambichler, M. Stucker, A. Orlikov, S. Rotterdam, P. Altmeyer, and K. Hoffmann, "Histomorphologic correlation with routine histology and optical coherence tomography," Skin Res. Technol. 10, 169-173 (2004).
[CrossRef] [PubMed]

N. D. Gladkova, G. A. Petrova, N. K. Nikulin, S. G. Radenska-Lopovok, L. B. Snopova, Y. P. Chumakov, V. A. Nasonova, V. M. Gelikonov, G. V. Gelikonov, R. V. Kuranov, A. M. Sergeev, and F. I. Feldchtein, "In vivo optical coherence tomography imaging of human skin: norm and pathology," Skin Res. Technol. 6, 6-16 (2000).
[CrossRef]

Other (2)

D. Hopwood, "Fixation and fixatives," in Theory and Practice of Histological Techniques, J.D.Bancroft and M.Gamble, eds. (Churchill Livingstone, 2002), pp. 71-72.

P. Nghiem, P. H. Mckee, and H. A. Haynes, "Merkel cell (cutaneous neuroendocrine) carcinoma," in Atlas of Clinical Oncology: Skin Cancer, A.J.Sober and F.H.Haluska, eds. (BC Decker Inc., 2001), pp. 132-133.

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (7)

Fig. 1
Fig. 1

Geometry of skin piercing and imaging.

Fig. 2
Fig. 2

(Color online) (a) Support steel ring being sewn to the abdomen of a pig. (b) OCT image being acquired during a second set of experiments from the tissue immediately after excision. The light dot of the red aiming beam on the tissue surface indicates the location of the OCT image plane.

Fig. 3
Fig. 3

(Color online) Histological images (a) with and (b) without a support ring. The black vertical bar corresponds to 1 mm. Light arrows indicate the dermis thickness.

Fig. 4
Fig. 4

Suture depth profiles derived from in vivo OCT images (curve 2) and from histological images (curve 1) in the tissue and their Gaussian fits (dashed). The profiles were acquired (a) with and (b) without a supporting ring. The origin of the x axis is the suture entrance through the skin surface.

Fig. 5
Fig. 5

(Color online) (a) OCT image and (b) corresponding histological image with a clearly seen suture track and a hair bulb. The black vertical bar corresponds to 1 mm.

Fig. 6
Fig. 6

(Color online) (a) OCT image and (b) corresponding histological image with a clearly seen suture track, hair bulb, and apocrinelike subdermal sweat glands. The black vertical bar corresponds to 1 mm.

Fig. 7
Fig. 7

(Color online) Histological images of neighboring tissue sites with three different apocrinelike subdermal sweat gland locations. The images correspond to tissue sections acquired by cutting tissue serially at 50 μm intervals.

Tables (1)

Tables Icon

Table 1 Changes in Dermis and Epithelium Thickness and in Maximal Suture Depth at Different Studies of Histological Processing a

Metrics