Abstract

We have designed, constructed, and tested a system that preselects the biological fraction of airborne particles from the overall aerosol. The preselection is based on fluorescence emission excited by a continuous 266nm laser beam. This beam is one of two cw beams used to measure the aerodynamic particle size of sampled particles. The intention in our system is that single particles, based on size and fluorescence emission, can be selected and further examined for chemical composition by mass spectrometry.

© 2006 Optical Society of America

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  1. M. P. Sinha, R. M. Platz, V. L. Vilker, and S. K. Friedlander, "Analysis of individual particles by mass spectrometry," Int. J. Mass Spectrom. Ion Processes 57, 125-133 (1984).
    [CrossRef]
  2. D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
    [CrossRef] [PubMed]
  3. K. T. Whitby, R. B. Husar, and B. Y. H. Lui, "The aerosol size distribution of Los Angeles smog," J. Coll. Interface Sci. 39, 177-204 (1972).
    [CrossRef]
  4. R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, "Fluorescence particle counter for detecting airborne bacteria and other biological particles," Aerosol. Sci. Technol. 23, 653-664 (1995).
    [CrossRef]
  5. Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
    [CrossRef]
  6. J. D. Eversole, W. K. Cary, Jr., C. S. Scotto, R. Pierson, M. Spence, and A. J. Campillo, "Continuous bioaerosol monitoring using UV excitation fluorescence:outdoor test results," Field Analyt. Chem. Technol. 15, 205-212 (2001).
    [CrossRef]
  7. Y. L. Pan, J. Hartings, R. G. Pinnick, S. C. Hill, J. Halverson, and R. K. Chang, "Single-particle fluorescence spectrometer for ambient aerosols particles," Aerosol Sci. Technol. 37, 628-639 (2003).
    [CrossRef]
  8. P. P. Hairston, J. Ho, and F. R. Quant, "Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence," J. Aerosol Sci. 28, 471-482 (1997).
    [CrossRef] [PubMed]
  9. Y. L. Pan, S. Holler, R. K. Chang, S. C. Hill, R. G. Pinnick, S. Niles, and J. R. Bottiger, "Single shot fluorescence spectra of individual micro-sized bio-aerosols illuminated by a 351 nm or 266 nm ultraviolet laser," Opt. Lett. 24, 116-119 (1999).
    [CrossRef]
  10. R. G. Pinnick, S. C. Hill, P. Nachman, G. Videen, G. Chen, and R. K. Chang, "Aerosol fluorescence spectrum analyzer for rapid measurement of single micrometer-sized airborne biological particles," Aerosol Sci. Technol. 28, 95-104 (1998).
    [CrossRef]
  11. S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
    [CrossRef]
  12. V. Sivaprakasam, A. L. Huston, C. Scotto, and J. D. Eversole, "Multiple UV wavelength excitation and fluorescence of bioaerosols," Opt. Express 12, 19 (2004).
    [CrossRef] [PubMed]
  13. J. Ho, "Future of biological aerosol detection," Anal. Chimica Acta 457, 125-148 (2002).
    [CrossRef]
  14. Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, "A puff of air sorts bioaerosol for pathogen identification," Aerosol Sci. Technol. 38, 598-602 (2004).
    [CrossRef]
  15. G. Chen, P. Nachman, R. G. Pinnick, S. C. Hill, and R. K. Chang, "Conditional-firing aerosol fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm laser excitation," Opt. Lett. 21, 1307-1309 (1996).
    [CrossRef] [PubMed]
  16. A. L. van Wuijckhuijse, M. A. Stowers, W. A. Kleefsman, B. L. M. van Baar, Ch. E. Kientz, and J. C. M. Marijnissen, "Matrix assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols: development of a fast detector for airborne biological pathogens," J. Aerosol Sci. 36, 677-687 (2005).
    [CrossRef]
  17. M. A. Stowers, A. L. van Wuijckhuijse, J. C. M. Marijnissen, B. Scarlett, B. L. M. van Baar, and Ch. E. Kientz, "Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry," Rapid Commun. Mass Spectrom. 14, 829-833 (2000).
    [CrossRef] [PubMed]
  18. P. H. Kaye, J. E. Barton, E. Hirst, and J. M. Clark, "Simultaneous light scattering and intrinsic fluorescence measurement for the classification of airborne particles," Appl. Opt. 39, 3738-3745 (2000).
    [CrossRef]
  19. R. G. Pinnick, S. C. Hill, Y. L. Pan, and R. K. Chang, "Fluorescence spectra of atmospheric aerosol at Adelphi, Maryland USA: measurement and classification of single particles containing organic carbon," Atmos. Environ. 38, 1657-1672 (2004).
    [CrossRef]

2005 (1)

A. L. van Wuijckhuijse, M. A. Stowers, W. A. Kleefsman, B. L. M. van Baar, Ch. E. Kientz, and J. C. M. Marijnissen, "Matrix assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols: development of a fast detector for airborne biological pathogens," J. Aerosol Sci. 36, 677-687 (2005).
[CrossRef]

2004 (4)

R. G. Pinnick, S. C. Hill, Y. L. Pan, and R. K. Chang, "Fluorescence spectra of atmospheric aerosol at Adelphi, Maryland USA: measurement and classification of single particles containing organic carbon," Atmos. Environ. 38, 1657-1672 (2004).
[CrossRef]

V. Sivaprakasam, A. L. Huston, C. Scotto, and J. D. Eversole, "Multiple UV wavelength excitation and fluorescence of bioaerosols," Opt. Express 12, 19 (2004).
[CrossRef] [PubMed]

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, "A puff of air sorts bioaerosol for pathogen identification," Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

2003 (1)

Y. L. Pan, J. Hartings, R. G. Pinnick, S. C. Hill, J. Halverson, and R. K. Chang, "Single-particle fluorescence spectrometer for ambient aerosols particles," Aerosol Sci. Technol. 37, 628-639 (2003).
[CrossRef]

2002 (1)

J. Ho, "Future of biological aerosol detection," Anal. Chimica Acta 457, 125-148 (2002).
[CrossRef]

2001 (1)

J. D. Eversole, W. K. Cary, Jr., C. S. Scotto, R. Pierson, M. Spence, and A. J. Campillo, "Continuous bioaerosol monitoring using UV excitation fluorescence:outdoor test results," Field Analyt. Chem. Technol. 15, 205-212 (2001).
[CrossRef]

2000 (2)

M. A. Stowers, A. L. van Wuijckhuijse, J. C. M. Marijnissen, B. Scarlett, B. L. M. van Baar, and Ch. E. Kientz, "Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry," Rapid Commun. Mass Spectrom. 14, 829-833 (2000).
[CrossRef] [PubMed]

P. H. Kaye, J. E. Barton, E. Hirst, and J. M. Clark, "Simultaneous light scattering and intrinsic fluorescence measurement for the classification of airborne particles," Appl. Opt. 39, 3738-3745 (2000).
[CrossRef]

1999 (3)

Y. L. Pan, S. Holler, R. K. Chang, S. C. Hill, R. G. Pinnick, S. Niles, and J. R. Bottiger, "Single shot fluorescence spectra of individual micro-sized bio-aerosols illuminated by a 351 nm or 266 nm ultraviolet laser," Opt. Lett. 24, 116-119 (1999).
[CrossRef]

S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
[CrossRef]

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

1998 (1)

R. G. Pinnick, S. C. Hill, P. Nachman, G. Videen, G. Chen, and R. K. Chang, "Aerosol fluorescence spectrum analyzer for rapid measurement of single micrometer-sized airborne biological particles," Aerosol Sci. Technol. 28, 95-104 (1998).
[CrossRef]

1997 (1)

P. P. Hairston, J. Ho, and F. R. Quant, "Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence," J. Aerosol Sci. 28, 471-482 (1997).
[CrossRef] [PubMed]

1996 (1)

1995 (1)

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, "Fluorescence particle counter for detecting airborne bacteria and other biological particles," Aerosol. Sci. Technol. 23, 653-664 (1995).
[CrossRef]

1984 (1)

M. P. Sinha, R. M. Platz, V. L. Vilker, and S. K. Friedlander, "Analysis of individual particles by mass spectrometry," Int. J. Mass Spectrom. Ion Processes 57, 125-133 (1984).
[CrossRef]

1972 (1)

K. T. Whitby, R. B. Husar, and B. Y. H. Lui, "The aerosol size distribution of Los Angeles smog," J. Coll. Interface Sci. 39, 177-204 (1972).
[CrossRef]

Barr, E. B.

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

Barton, J. E.

Bottiger, J.

S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
[CrossRef]

Bottiger, J. R.

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, "A puff of air sorts bioaerosol for pathogen identification," Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

Y. L. Pan, S. Holler, R. K. Chang, S. C. Hill, R. G. Pinnick, S. Niles, and J. R. Bottiger, "Single shot fluorescence spectra of individual micro-sized bio-aerosols illuminated by a 351 nm or 266 nm ultraviolet laser," Opt. Lett. 24, 116-119 (1999).
[CrossRef]

Boutou, V.

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, "A puff of air sorts bioaerosol for pathogen identification," Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

Bruno, J. G.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, "Fluorescence particle counter for detecting airborne bacteria and other biological particles," Aerosol. Sci. Technol. 23, 653-664 (1995).
[CrossRef]

Campillo, A. J.

J. D. Eversole, W. K. Cary, Jr., C. S. Scotto, R. Pierson, M. Spence, and A. J. Campillo, "Continuous bioaerosol monitoring using UV excitation fluorescence:outdoor test results," Field Analyt. Chem. Technol. 15, 205-212 (2001).
[CrossRef]

Cary, W. K.

J. D. Eversole, W. K. Cary, Jr., C. S. Scotto, R. Pierson, M. Spence, and A. J. Campillo, "Continuous bioaerosol monitoring using UV excitation fluorescence:outdoor test results," Field Analyt. Chem. Technol. 15, 205-212 (2001).
[CrossRef]

Chang, J. T.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Chang, R. K.

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, "A puff of air sorts bioaerosol for pathogen identification," Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

R. G. Pinnick, S. C. Hill, Y. L. Pan, and R. K. Chang, "Fluorescence spectra of atmospheric aerosol at Adelphi, Maryland USA: measurement and classification of single particles containing organic carbon," Atmos. Environ. 38, 1657-1672 (2004).
[CrossRef]

Y. L. Pan, J. Hartings, R. G. Pinnick, S. C. Hill, J. Halverson, and R. K. Chang, "Single-particle fluorescence spectrometer for ambient aerosols particles," Aerosol Sci. Technol. 37, 628-639 (2003).
[CrossRef]

S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
[CrossRef]

Y. L. Pan, S. Holler, R. K. Chang, S. C. Hill, R. G. Pinnick, S. Niles, and J. R. Bottiger, "Single shot fluorescence spectra of individual micro-sized bio-aerosols illuminated by a 351 nm or 266 nm ultraviolet laser," Opt. Lett. 24, 116-119 (1999).
[CrossRef]

R. G. Pinnick, S. C. Hill, P. Nachman, G. Videen, G. Chen, and R. K. Chang, "Aerosol fluorescence spectrum analyzer for rapid measurement of single micrometer-sized airborne biological particles," Aerosol Sci. Technol. 28, 95-104 (1998).
[CrossRef]

G. Chen, P. Nachman, R. G. Pinnick, S. C. Hill, and R. K. Chang, "Conditional-firing aerosol fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm laser excitation," Opt. Lett. 21, 1307-1309 (1996).
[CrossRef] [PubMed]

Chen, B. T.

S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
[CrossRef]

Chen, G.

R. G. Pinnick, S. C. Hill, P. Nachman, G. Videen, G. Chen, and R. K. Chang, "Aerosol fluorescence spectrum analyzer for rapid measurement of single micrometer-sized airborne biological particles," Aerosol Sci. Technol. 28, 95-104 (1998).
[CrossRef]

G. Chen, P. Nachman, R. G. Pinnick, S. C. Hill, and R. K. Chang, "Conditional-firing aerosol fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm laser excitation," Opt. Lett. 21, 1307-1309 (1996).
[CrossRef] [PubMed]

Cheng, Y. S.

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

Clark, J. M.

Coffee, K. R.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Czerwieniec, G. A.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Estacio, P. L.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Eversole, J. D.

V. Sivaprakasam, A. L. Huston, C. Scotto, and J. D. Eversole, "Multiple UV wavelength excitation and fluorescence of bioaerosols," Opt. Express 12, 19 (2004).
[CrossRef] [PubMed]

J. D. Eversole, W. K. Cary, Jr., C. S. Scotto, R. Pierson, M. Spence, and A. J. Campillo, "Continuous bioaerosol monitoring using UV excitation fluorescence:outdoor test results," Field Analyt. Chem. Technol. 15, 205-212 (2001).
[CrossRef]

Fan, B. J.

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

Feather, G.

S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
[CrossRef]

Fergenson, D. P.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Fernandez, G. L.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, "Fluorescence particle counter for detecting airborne bacteria and other biological particles," Aerosol. Sci. Technol. 23, 653-664 (1995).
[CrossRef]

Frank, M.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Friedlander, S. K.

M. P. Sinha, R. M. Platz, V. L. Vilker, and S. K. Friedlander, "Analysis of individual particles by mass spectrometry," Int. J. Mass Spectrom. Ion Processes 57, 125-133 (1984).
[CrossRef]

Gard, E. E.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Hadley, R. T.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Hairston, P. P.

P. P. Hairston, J. Ho, and F. R. Quant, "Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence," J. Aerosol Sci. 28, 471-482 (1997).
[CrossRef] [PubMed]

Hall, H. L.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Halverson, J.

Y. L. Pan, J. Hartings, R. G. Pinnick, S. C. Hill, J. Halverson, and R. K. Chang, "Single-particle fluorescence spectrometer for ambient aerosols particles," Aerosol Sci. Technol. 37, 628-639 (2003).
[CrossRef]

Hargis, P. J.

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

Hartings, J.

Y. L. Pan, J. Hartings, R. G. Pinnick, S. C. Hill, J. Halverson, and R. K. Chang, "Single-particle fluorescence spectrometer for ambient aerosols particles," Aerosol Sci. Technol. 37, 628-639 (2003).
[CrossRef]

Hill, S. C.

R. G. Pinnick, S. C. Hill, Y. L. Pan, and R. K. Chang, "Fluorescence spectra of atmospheric aerosol at Adelphi, Maryland USA: measurement and classification of single particles containing organic carbon," Atmos. Environ. 38, 1657-1672 (2004).
[CrossRef]

Y. L. Pan, J. Hartings, R. G. Pinnick, S. C. Hill, J. Halverson, and R. K. Chang, "Single-particle fluorescence spectrometer for ambient aerosols particles," Aerosol Sci. Technol. 37, 628-639 (2003).
[CrossRef]

S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
[CrossRef]

Y. L. Pan, S. Holler, R. K. Chang, S. C. Hill, R. G. Pinnick, S. Niles, and J. R. Bottiger, "Single shot fluorescence spectra of individual micro-sized bio-aerosols illuminated by a 351 nm or 266 nm ultraviolet laser," Opt. Lett. 24, 116-119 (1999).
[CrossRef]

R. G. Pinnick, S. C. Hill, P. Nachman, G. Videen, G. Chen, and R. K. Chang, "Aerosol fluorescence spectrum analyzer for rapid measurement of single micrometer-sized airborne biological particles," Aerosol Sci. Technol. 28, 95-104 (1998).
[CrossRef]

G. Chen, P. Nachman, R. G. Pinnick, S. C. Hill, and R. K. Chang, "Conditional-firing aerosol fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm laser excitation," Opt. Lett. 21, 1307-1309 (1996).
[CrossRef] [PubMed]

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, "Fluorescence particle counter for detecting airborne bacteria and other biological particles," Aerosol. Sci. Technol. 23, 653-664 (1995).
[CrossRef]

Hirst, E.

Ho, J.

J. Ho, "Future of biological aerosol detection," Anal. Chimica Acta 457, 125-148 (2002).
[CrossRef]

P. P. Hairston, J. Ho, and F. R. Quant, "Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence," J. Aerosol Sci. 28, 471-482 (1997).
[CrossRef] [PubMed]

Holler, S.

S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
[CrossRef]

Y. L. Pan, S. Holler, R. K. Chang, S. C. Hill, R. G. Pinnick, S. Niles, and J. R. Bottiger, "Single shot fluorescence spectra of individual micro-sized bio-aerosols illuminated by a 351 nm or 266 nm ultraviolet laser," Opt. Lett. 24, 116-119 (1999).
[CrossRef]

Horn, J. M.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Husar, R. B.

K. T. Whitby, R. B. Husar, and B. Y. H. Lui, "The aerosol size distribution of Los Angeles smog," J. Coll. Interface Sci. 39, 177-204 (1972).
[CrossRef]

Huston, A. L.

V. Sivaprakasam, A. L. Huston, C. Scotto, and J. D. Eversole, "Multiple UV wavelength excitation and fluorescence of bioaerosols," Opt. Express 12, 19 (2004).
[CrossRef] [PubMed]

Kaye, P. H.

Kientz, Ch. E.

A. L. van Wuijckhuijse, M. A. Stowers, W. A. Kleefsman, B. L. M. van Baar, Ch. E. Kientz, and J. C. M. Marijnissen, "Matrix assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols: development of a fast detector for airborne biological pathogens," J. Aerosol Sci. 36, 677-687 (2005).
[CrossRef]

M. A. Stowers, A. L. van Wuijckhuijse, J. C. M. Marijnissen, B. Scarlett, B. L. M. van Baar, and Ch. E. Kientz, "Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry," Rapid Commun. Mass Spectrom. 14, 829-833 (2000).
[CrossRef] [PubMed]

Kleefsman, W. A.

A. L. van Wuijckhuijse, M. A. Stowers, W. A. Kleefsman, B. L. M. van Baar, Ch. E. Kientz, and J. C. M. Marijnissen, "Matrix assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols: development of a fast detector for airborne biological pathogens," J. Aerosol Sci. 36, 677-687 (2005).
[CrossRef]

Langlois, R. G.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Lebrilla, C. B.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Lui, B. Y. H.

K. T. Whitby, R. B. Husar, and B. Y. H. Lui, "The aerosol size distribution of Los Angeles smog," J. Coll. Interface Sci. 39, 177-204 (1972).
[CrossRef]

Marijnissen, J. C. M.

A. L. van Wuijckhuijse, M. A. Stowers, W. A. Kleefsman, B. L. M. van Baar, Ch. E. Kientz, and J. C. M. Marijnissen, "Matrix assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols: development of a fast detector for airborne biological pathogens," J. Aerosol Sci. 36, 677-687 (2005).
[CrossRef]

M. A. Stowers, A. L. van Wuijckhuijse, J. C. M. Marijnissen, B. Scarlett, B. L. M. van Baar, and Ch. E. Kientz, "Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry," Rapid Commun. Mass Spectrom. 14, 829-833 (2000).
[CrossRef] [PubMed]

Mayo, M. W.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, "Fluorescence particle counter for detecting airborne bacteria and other biological particles," Aerosol. Sci. Technol. 23, 653-664 (1995).
[CrossRef]

Nachman, P.

R. G. Pinnick, S. C. Hill, P. Nachman, G. Videen, G. Chen, and R. K. Chang, "Aerosol fluorescence spectrum analyzer for rapid measurement of single micrometer-sized airborne biological particles," Aerosol Sci. Technol. 28, 95-104 (1998).
[CrossRef]

G. Chen, P. Nachman, R. G. Pinnick, S. C. Hill, and R. K. Chang, "Conditional-firing aerosol fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm laser excitation," Opt. Lett. 21, 1307-1309 (1996).
[CrossRef] [PubMed]

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, "Fluorescence particle counter for detecting airborne bacteria and other biological particles," Aerosol. Sci. Technol. 23, 653-664 (1995).
[CrossRef]

Niles, S.

S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
[CrossRef]

Y. L. Pan, S. Holler, R. K. Chang, S. C. Hill, R. G. Pinnick, S. Niles, and J. R. Bottiger, "Single shot fluorescence spectra of individual micro-sized bio-aerosols illuminated by a 351 nm or 266 nm ultraviolet laser," Opt. Lett. 24, 116-119 (1999).
[CrossRef]

O'Hern, T. J.

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

Orr, C. S.

S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
[CrossRef]

Pan, Y. L.

R. G. Pinnick, S. C. Hill, Y. L. Pan, and R. K. Chang, "Fluorescence spectra of atmospheric aerosol at Adelphi, Maryland USA: measurement and classification of single particles containing organic carbon," Atmos. Environ. 38, 1657-1672 (2004).
[CrossRef]

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, "A puff of air sorts bioaerosol for pathogen identification," Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

Y. L. Pan, J. Hartings, R. G. Pinnick, S. C. Hill, J. Halverson, and R. K. Chang, "Single-particle fluorescence spectrometer for ambient aerosols particles," Aerosol Sci. Technol. 37, 628-639 (2003).
[CrossRef]

S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
[CrossRef]

Y. L. Pan, S. Holler, R. K. Chang, S. C. Hill, R. G. Pinnick, S. Niles, and J. R. Bottiger, "Single shot fluorescence spectra of individual micro-sized bio-aerosols illuminated by a 351 nm or 266 nm ultraviolet laser," Opt. Lett. 24, 116-119 (1999).
[CrossRef]

Pendleton, J. D.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, "Fluorescence particle counter for detecting airborne bacteria and other biological particles," Aerosol. Sci. Technol. 23, 653-664 (1995).
[CrossRef]

Pierson, R.

J. D. Eversole, W. K. Cary, Jr., C. S. Scotto, R. Pierson, M. Spence, and A. J. Campillo, "Continuous bioaerosol monitoring using UV excitation fluorescence:outdoor test results," Field Analyt. Chem. Technol. 15, 205-212 (2001).
[CrossRef]

Pillai, S. P.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Pinnick, R. G.

R. G. Pinnick, S. C. Hill, Y. L. Pan, and R. K. Chang, "Fluorescence spectra of atmospheric aerosol at Adelphi, Maryland USA: measurement and classification of single particles containing organic carbon," Atmos. Environ. 38, 1657-1672 (2004).
[CrossRef]

Y. L. Pan, J. Hartings, R. G. Pinnick, S. C. Hill, J. Halverson, and R. K. Chang, "Single-particle fluorescence spectrometer for ambient aerosols particles," Aerosol Sci. Technol. 37, 628-639 (2003).
[CrossRef]

S. C. Hill, R. G. Pinnick, S. Niles, Y. L. Pan, S. Holler, R. K. Chang, J. Bottiger, B. T. Chen, C. S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999).
[CrossRef]

Y. L. Pan, S. Holler, R. K. Chang, S. C. Hill, R. G. Pinnick, S. Niles, and J. R. Bottiger, "Single shot fluorescence spectra of individual micro-sized bio-aerosols illuminated by a 351 nm or 266 nm ultraviolet laser," Opt. Lett. 24, 116-119 (1999).
[CrossRef]

R. G. Pinnick, S. C. Hill, P. Nachman, G. Videen, G. Chen, and R. K. Chang, "Aerosol fluorescence spectrum analyzer for rapid measurement of single micrometer-sized airborne biological particles," Aerosol Sci. Technol. 28, 95-104 (1998).
[CrossRef]

G. Chen, P. Nachman, R. G. Pinnick, S. C. Hill, and R. K. Chang, "Conditional-firing aerosol fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm laser excitation," Opt. Lett. 21, 1307-1309 (1996).
[CrossRef] [PubMed]

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, "Fluorescence particle counter for detecting airborne bacteria and other biological particles," Aerosol. Sci. Technol. 23, 653-664 (1995).
[CrossRef]

Pitesky, M. E.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Platz, R. M.

M. P. Sinha, R. M. Platz, V. L. Vilker, and S. K. Friedlander, "Analysis of individual particles by mass spectrometry," Int. J. Mass Spectrom. Ion Processes 57, 125-133 (1984).
[CrossRef]

Preppernau, B. L.

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

Quant, F. R.

P. P. Hairston, J. Ho, and F. R. Quant, "Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence," J. Aerosol Sci. 28, 471-482 (1997).
[CrossRef] [PubMed]

Rader, D. J.

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

Radloff, R. J.

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

Ramponi, A. J.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Russell, S. C.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Scarlett, B.

M. A. Stowers, A. L. van Wuijckhuijse, J. C. M. Marijnissen, B. Scarlett, B. L. M. van Baar, and Ch. E. Kientz, "Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry," Rapid Commun. Mass Spectrom. 14, 829-833 (2000).
[CrossRef] [PubMed]

Scotto, C.

V. Sivaprakasam, A. L. Huston, C. Scotto, and J. D. Eversole, "Multiple UV wavelength excitation and fluorescence of bioaerosols," Opt. Express 12, 19 (2004).
[CrossRef] [PubMed]

Scotto, C. S.

J. D. Eversole, W. K. Cary, Jr., C. S. Scotto, R. Pierson, M. Spence, and A. J. Campillo, "Continuous bioaerosol monitoring using UV excitation fluorescence:outdoor test results," Field Analyt. Chem. Technol. 15, 205-212 (2001).
[CrossRef]

Shih, M. T. P.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Sinha, M. P.

M. P. Sinha, R. M. Platz, V. L. Vilker, and S. K. Friedlander, "Analysis of individual particles by mass spectrometry," Int. J. Mass Spectrom. Ion Processes 57, 125-133 (1984).
[CrossRef]

Sivaprakasam, V.

V. Sivaprakasam, A. L. Huston, C. Scotto, and J. D. Eversole, "Multiple UV wavelength excitation and fluorescence of bioaerosols," Opt. Express 12, 19 (2004).
[CrossRef] [PubMed]

Spence, M.

J. D. Eversole, W. K. Cary, Jr., C. S. Scotto, R. Pierson, M. Spence, and A. J. Campillo, "Continuous bioaerosol monitoring using UV excitation fluorescence:outdoor test results," Field Analyt. Chem. Technol. 15, 205-212 (2001).
[CrossRef]

Srivastava, A.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Steele, P. T.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Stowers, M. A.

A. L. van Wuijckhuijse, M. A. Stowers, W. A. Kleefsman, B. L. M. van Baar, Ch. E. Kientz, and J. C. M. Marijnissen, "Matrix assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols: development of a fast detector for airborne biological pathogens," J. Aerosol Sci. 36, 677-687 (2005).
[CrossRef]

M. A. Stowers, A. L. van Wuijckhuijse, J. C. M. Marijnissen, B. Scarlett, B. L. M. van Baar, and Ch. E. Kientz, "Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry," Rapid Commun. Mass Spectrom. 14, 829-833 (2000).
[CrossRef] [PubMed]

Tisone, G. C.

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

Tobias, H. J.

D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
[CrossRef] [PubMed]

Torczynski, J. R.

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

van Baar, B. L. M.

A. L. van Wuijckhuijse, M. A. Stowers, W. A. Kleefsman, B. L. M. van Baar, Ch. E. Kientz, and J. C. M. Marijnissen, "Matrix assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols: development of a fast detector for airborne biological pathogens," J. Aerosol Sci. 36, 677-687 (2005).
[CrossRef]

M. A. Stowers, A. L. van Wuijckhuijse, J. C. M. Marijnissen, B. Scarlett, B. L. M. van Baar, and Ch. E. Kientz, "Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry," Rapid Commun. Mass Spectrom. 14, 829-833 (2000).
[CrossRef] [PubMed]

van Wuijckhuijse, A. L.

A. L. van Wuijckhuijse, M. A. Stowers, W. A. Kleefsman, B. L. M. van Baar, Ch. E. Kientz, and J. C. M. Marijnissen, "Matrix assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols: development of a fast detector for airborne biological pathogens," J. Aerosol Sci. 36, 677-687 (2005).
[CrossRef]

M. A. Stowers, A. L. van Wuijckhuijse, J. C. M. Marijnissen, B. Scarlett, B. L. M. van Baar, and Ch. E. Kientz, "Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry," Rapid Commun. Mass Spectrom. 14, 829-833 (2000).
[CrossRef] [PubMed]

Videen, G.

R. G. Pinnick, S. C. Hill, P. Nachman, G. Videen, G. Chen, and R. K. Chang, "Aerosol fluorescence spectrum analyzer for rapid measurement of single micrometer-sized airborne biological particles," Aerosol Sci. Technol. 28, 95-104 (1998).
[CrossRef]

Vilker, V. L.

M. P. Sinha, R. M. Platz, V. L. Vilker, and S. K. Friedlander, "Analysis of individual particles by mass spectrometry," Int. J. Mass Spectrom. Ion Processes 57, 125-133 (1984).
[CrossRef]

Whitby, K. T.

K. T. Whitby, R. B. Husar, and B. Y. H. Lui, "The aerosol size distribution of Los Angeles smog," J. Coll. Interface Sci. 39, 177-204 (1972).
[CrossRef]

Wolf, J. P.

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, "A puff of air sorts bioaerosol for pathogen identification," Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

Young, S. A.

Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernau, S. A. Young, R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999).
[CrossRef]

Zhang, S. S.

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, "A puff of air sorts bioaerosol for pathogen identification," Aerosol Sci. Technol. 38, 598-602 (2004).
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R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, "Fluorescence particle counter for detecting airborne bacteria and other biological particles," Aerosol. Sci. Technol. 23, 653-664 (1995).
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D. P. Fergenson, M. E. Pitesky, H. J. Tobias, P. T. Steele, G. A. Czerwieniec, S. C. Russell, C. B. Lebrilla, J. M. Horn, K. R. Coffee, A. Srivastava, S. P. Pillai, M. T. P. Shih, H. L. Hall, A. J. Ramponi, J. T. Chang, R. G. Langlois, P. L. Estacio, R. T. Hadley, M. Frank, and E. E. Gard, "Reagentless detection and classification of individual bioaerosol particles in seconds," Anal. Chem. 76, 373-378 (2004).
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R. G. Pinnick, S. C. Hill, Y. L. Pan, and R. K. Chang, "Fluorescence spectra of atmospheric aerosol at Adelphi, Maryland USA: measurement and classification of single particles containing organic carbon," Atmos. Environ. 38, 1657-1672 (2004).
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Figures (5)

Fig. 1
Fig. 1

Schematic diagram of the system.

Fig. 2
Fig. 2

Single-particle fluorescence spectra obtained from particles containing tryptophan and three species of bacteria cells.

Fig. 3
Fig. 3

Particle aerodynamic size/fluorescence intensity histogram for a series of polystyrene latex spheres.

Fig. 4
Fig. 4

The aerodynamic size/fluorescence intensity histograms generated by sampling an aerosol produced from unwashed B. globigii spores using (a) one-color detection and (b) two-color detection.

Fig. 5
Fig. 5

Consecutive single-particle mass spectra collected from an externally mixed aerosol containing lead chloride particles and tryptophan particles using (a) one-color detection and (b) two-color detection.

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