Abstract

Common-path phase-shifting lensless holographic microscopy has been recently proposed as a novel approach capable of high numerical aperture imaging in a lensless digital inline holographic microscopy layout [Opt. Lett. 35, 3919 (2010)]. Here we present proof-of-concept validation for improving the resolution limit imposed by diffraction in such a setup. This is accomplished by shifting the phase lens displayed at the spatial light modulator, which moves the illumination point source to different off-axis positions. For each off-axis position, a set of inline phase-shifted holograms are recorded by the digital sensor and stored at the computer’s memory for later digital postprocessing. As a consequence, each recording allows the recovery of different spatial frequency content of the object’s diffracted wavefront meaning a superresolved image of the input object. Experimental results are reported validating the proposed method.

© 2012 Optical Society of America

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2012

2011

2010

2009

2008

A. Ozcan and U. Demirci, Lab Chip 8, 98 (2008).
[CrossRef]

2006

2004

2003

2001

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

1948

D. Gabor, Nature 161, 777 (1948).
[CrossRef]

Allano, D.

Alvarez-Palacio, D. C.

Bishara, W.

Born, M.

M. Born and E. Wolf, Principles of Optics, 7th (expanded) ed. (Cambridge University, 1999).

Coëtmellec, S.

Coskun, A. F.

Demirci, U.

A. Ozcan and U. Demirci, Lab Chip 8, 98 (2008).
[CrossRef]

Gabor, D.

D. Gabor, Nature 161, 777 (1948).
[CrossRef]

Gao, P.

Garcia, J.

García, J.

Garcia-Sucerquia, J.

Granero, L.

L. Granero, V. Micó, Z. Zalevsky, and J. García, Appl. Opt. 49, 845 (2010).
[CrossRef]

V. Micó, L. Granero, Z. Zalevsky, and J. García, J. Opt. A 11, 125408 (2009).
[CrossRef]

Graulig, C.

Gur, E.

Harder, I.

Javidi, B.

Jericho, M. H.

J. Garcia-Sucerquia, D. C. Alvarez-Palacio, M. H. Jericho, and H. J. Kreuzer, Opt. Lett. 31, 2845 (2006).
[CrossRef]

S. K. Jericho, J. Garcia-Sucerquia, W. Xu, M. H. Jericho, and H. J. Kreuzer, Rev. Sci. Instrum. 77, 043706 (2006).
[CrossRef]

J. Garcia-Sucerquia, W. Xu, M. H. Jericho, and H. J. Kreuzer, Opt. Lett. 31, 1211 (2006).
[CrossRef]

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Opt. Lett. 28, 164 (2003).
[CrossRef]

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Jericho, S. K.

S. K. Jericho, J. Garcia-Sucerquia, W. Xu, M. H. Jericho, and H. J. Kreuzer, Rev. Sci. Instrum. 77, 043706 (2006).
[CrossRef]

Kanka, M.

Kreuzer, H. J.

Lebrun, D.

Lindlein, N.

Malek, M.

Mantel, K.

Meinertzhagen, I. A.

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Opt. Lett. 28, 164 (2003).
[CrossRef]

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Micó, V.

Nercissian, V.

Ozcan, A.

Petruck, P.

Piano, E.

Pontiggia, C.

Repetto, L.

Restrepo, J. F.

Riesenberg, R.

Su, T. W.

Torcal-Milla, F. J.

Wolf, E.

M. Born and E. Wolf, Principles of Optics, 7th (expanded) ed. (Cambridge University, 1999).

Xu, W.

J. Garcia-Sucerquia, W. Xu, M. H. Jericho, and H. J. Kreuzer, Opt. Lett. 31, 1211 (2006).
[CrossRef]

S. K. Jericho, J. Garcia-Sucerquia, W. Xu, M. H. Jericho, and H. J. Kreuzer, Rev. Sci. Instrum. 77, 043706 (2006).
[CrossRef]

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Opt. Lett. 28, 164 (2003).
[CrossRef]

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Yao, B.

Zalevsky, Z.

Zhu, H.

Appl. Opt.

J. Biomed. Opt.

V. Micó and Z. Zalevsky, J. Biomed. Opt. 15, 046027 (2010).
[CrossRef]

J. Opt. A

V. Micó, L. Granero, Z. Zalevsky, and J. García, J. Opt. A 11, 125408 (2009).
[CrossRef]

Lab Chip

A. Ozcan and U. Demirci, Lab Chip 8, 98 (2008).
[CrossRef]

Nature

D. Gabor, Nature 161, 777 (1948).
[CrossRef]

Opt. Express

Opt. Lett.

Proc. Natl. Acad. Sci. USA

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Rev. Sci. Instrum.

S. K. Jericho, J. Garcia-Sucerquia, W. Xu, M. H. Jericho, and H. J. Kreuzer, Rev. Sci. Instrum. 77, 043706 (2006).
[CrossRef]

Other

M. Born and E. Wolf, Principles of Optics, 7th (expanded) ed. (Cambridge University, 1999).

Supplementary Material (1)

» Media 1: MOV (2419 KB)     

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Figures (4)

Fig. 1.
Fig. 1.

Experimental setup of the proposed common-path phase-shifting DIHM configuration with (a) on-axis and (b) off-axis illuminations.

Fig. 2.
Fig. 2.

Retrieved complex amplitude images and the full set of inline holograms (Media 1) for the five image spot positions.

Fig. 3.
Fig. 3.

Final images obtained under common-path phase-shifting DIHM when (a) on-axis (low-resolution image) and (b) tilted beam multiplexing (superresolved image) illuminations are used.

Fig. 4.
Fig. 4.

Second experiment for the object placed at z1=5.8mm: (a) full image and (b) magnification of the dashed white rectangle of (a) showing the same field of view as shown in Fig. 3(b) and 3(c) the superresolved image again [same as in Fig. 3(b)] for comparison.

Metrics