Abstract

Color digital lensless holographic microscopy with micrometer resolution is presented. Multiwavelength illumination of a biological sample and a posteriori color composition of the amplitude images individually reconstructed are used to obtain full-color representation of the microscopic specimen. To match the sizes of the reconstructed holograms for each wavelength, a reconstruction algorithm that allows for choosing the pixel size at the reconstruction plane independently of the wavelength and the reconstruction distance is used. The method is illustrated with experimental results.

© 2012 Optical Society of America

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2011

2010

T. Tahara, T. Kakue, Y. Awatsuji, K. Nishio, S. Ura, T. Kubota, and O. Matoba, 3D Res 1 (4), 1 (2010).
[CrossRef]

S. O. Isikman, I. Sencan, O. Mudanyali, W. Bishara, C. Oztoprak, and A. Ozcan, Lab Chip 10, 1109 (2010).

2009

X. Mo, B. Kemper, P. Langehanenberg, A. Vollmer, J. Xie, and G. vonBally, Proc. SPIE 7367, 736718 (2009).
[CrossRef]

T. Kakue, T. Tahara, K. Ito, Y. Shimozato, Y. Awatsuji, K. Nishio, S. Ura, T. Kubota, and O. Matoba, Appl. Opt. 48, H244 (2009).
[CrossRef]

2008

P. Picart, D. Mounier, and J. Michel Desse, Opt. Lett. 33, 276 (2008).
[CrossRef]

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. Nicola, A. Finizio, and B. Javidi, J. Disp. Technol. 4, 97 (2008).
[CrossRef]

2006

2003

2002

Alfieri, D.

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. Nicola, A. Finizio, and B. Javidi, J. Disp. Technol. 4, 97 (2008).
[CrossRef]

Andrés, P.

Araiza-Esquivel, M. A.

Awatsuji, Y.

Bishara, W.

S. O. Isikman, I. Sencan, O. Mudanyali, W. Bishara, C. Oztoprak, and A. Ozcan, Lab Chip 10, 1109 (2010).

Demoli, N.

Do, C.

B. Javidi, C. Do, S. Hong, and T. Nomura, J. Disp. Technol. 2, 411 (2006).
[CrossRef]

Ferraro, P.

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. Nicola, A. Finizio, and B. Javidi, J. Disp. Technol. 4, 97 (2008).
[CrossRef]

Finizio, A.

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. Nicola, A. Finizio, and B. Javidi, J. Disp. Technol. 4, 97 (2008).
[CrossRef]

Garcia-Sucerquia, J.

Grilli, S.

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. Nicola, A. Finizio, and B. Javidi, J. Disp. Technol. 4, 97 (2008).
[CrossRef]

Hong, S.

B. Javidi, C. Do, S. Hong, and T. Nomura, J. Disp. Technol. 2, 411 (2006).
[CrossRef]

Isikman, S. O.

S. O. Isikman, I. Sencan, O. Mudanyali, W. Bishara, C. Oztoprak, and A. Ozcan, Lab Chip 10, 1109 (2010).

Ito, K.

Ito, Y.

Javidi, B.

M. A. Araiza-Esquivel, L. Martínez-León, B. Javidi, P. Andrés, J. Lancis, and E. Tajahuerce, Appl. Opt. 50, B96 (2011).
[CrossRef]

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. Nicola, A. Finizio, and B. Javidi, J. Disp. Technol. 4, 97 (2008).
[CrossRef]

B. Javidi, C. Do, S. Hong, and T. Nomura, J. Disp. Technol. 2, 411 (2006).
[CrossRef]

Jericho, M. H.

J. Garcia-Sucerquia, W. Xu, P. Klages, S. M. Jericho, M. H. Jericho, and H. J. Kreuzer, Appl. Opt. 45, 836 (2006).
[CrossRef]

M. H. Jericho and H. J. Kreuzer, in Coherent Light Microscopy, P. P. Ferraro, A. Wax, and Z. Zalevvsky, eds. (Springer Series in Surface Sciences, 2011), pp. 3–30.

Jericho, S. M.

Kakue, T.

Kato, J. I.

Kemper, B.

X. Mo, B. Kemper, P. Langehanenberg, A. Vollmer, J. Xie, and G. vonBally, Proc. SPIE 7367, 736718 (2009).
[CrossRef]

Klages, P.

Kreuzer, H. J.

J. Garcia-Sucerquia, W. Xu, P. Klages, S. M. Jericho, M. H. Jericho, and H. J. Kreuzer, Appl. Opt. 45, 836 (2006).
[CrossRef]

H. J. Kreuzer, “Holographic microscope and method of hologram reconstruction,” U.S. patent 6,411,406 B1 (June25, 2002).

M. H. Jericho and H. J. Kreuzer, in Coherent Light Microscopy, P. P. Ferraro, A. Wax, and Z. Zalevvsky, eds. (Springer Series in Surface Sciences, 2011), pp. 3–30.

Kubota, T.

Lancis, J.

Langehanenberg, P.

X. Mo, B. Kemper, P. Langehanenberg, A. Vollmer, J. Xie, and G. vonBally, Proc. SPIE 7367, 736718 (2009).
[CrossRef]

Martínez-León, L.

Matoba, O.

Matsumura, T.

Miccio, L.

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. Nicola, A. Finizio, and B. Javidi, J. Disp. Technol. 4, 97 (2008).
[CrossRef]

Michel Desse, J.

Mo, X.

X. Mo, B. Kemper, P. Langehanenberg, A. Vollmer, J. Xie, and G. vonBally, Proc. SPIE 7367, 736718 (2009).
[CrossRef]

Mounier, D.

Mudanyali, O.

S. O. Isikman, I. Sencan, O. Mudanyali, W. Bishara, C. Oztoprak, and A. Ozcan, Lab Chip 10, 1109 (2010).

Nicola, S.

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. Nicola, A. Finizio, and B. Javidi, J. Disp. Technol. 4, 97 (2008).
[CrossRef]

Nishio, K.

Nomura, T.

B. Javidi, C. Do, S. Hong, and T. Nomura, J. Disp. Technol. 2, 411 (2006).
[CrossRef]

Ozcan, A.

S. O. Isikman, I. Sencan, O. Mudanyali, W. Bishara, C. Oztoprak, and A. Ozcan, Lab Chip 10, 1109 (2010).

Oztoprak, C.

S. O. Isikman, I. Sencan, O. Mudanyali, W. Bishara, C. Oztoprak, and A. Ozcan, Lab Chip 10, 1109 (2010).

Picart, P.

Sencan, I.

S. O. Isikman, I. Sencan, O. Mudanyali, W. Bishara, C. Oztoprak, and A. Ozcan, Lab Chip 10, 1109 (2010).

Shimozato, Y.

Tahara, T.

Tajahuerce, E.

Torzynski, M.

Ura, S.

Vollmer, A.

X. Mo, B. Kemper, P. Langehanenberg, A. Vollmer, J. Xie, and G. vonBally, Proc. SPIE 7367, 736718 (2009).
[CrossRef]

vonBally, G.

X. Mo, B. Kemper, P. Langehanenberg, A. Vollmer, J. Xie, and G. vonBally, Proc. SPIE 7367, 736718 (2009).
[CrossRef]

Vukicevic, D.

Xia, P.

Xie, J.

X. Mo, B. Kemper, P. Langehanenberg, A. Vollmer, J. Xie, and G. vonBally, Proc. SPIE 7367, 736718 (2009).
[CrossRef]

Xu, W.

Yamaguchi, I.

3D Res

T. Tahara, T. Kakue, Y. Awatsuji, K. Nishio, S. Ura, T. Kubota, and O. Matoba, 3D Res 1 (4), 1 (2010).
[CrossRef]

Appl. Opt.

J. Disp. Technol.

B. Javidi, C. Do, S. Hong, and T. Nomura, J. Disp. Technol. 2, 411 (2006).
[CrossRef]

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. Nicola, A. Finizio, and B. Javidi, J. Disp. Technol. 4, 97 (2008).
[CrossRef]

Lab Chip

S. O. Isikman, I. Sencan, O. Mudanyali, W. Bishara, C. Oztoprak, and A. Ozcan, Lab Chip 10, 1109 (2010).

Opt. Express

Opt. Lett.

Proc. SPIE

X. Mo, B. Kemper, P. Langehanenberg, A. Vollmer, J. Xie, and G. vonBally, Proc. SPIE 7367, 736718 (2009).
[CrossRef]

Other

M. H. Jericho and H. J. Kreuzer, in Coherent Light Microscopy, P. P. Ferraro, A. Wax, and Z. Zalevvsky, eds. (Springer Series in Surface Sciences, 2011), pp. 3–30.

H. J. Kreuzer, “Holographic microscope and method of hologram reconstruction,” U.S. patent 6,411,406 B1 (June25, 2002).

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Figures (3)

Fig. 1.
Fig. 1.

Illustration of CDLHM. The three lasers are aligned with the use of dichroic beam combiners. All the laser beams are focused down onto a pinhole by means of a plan apochromat microscope objective. Sequential shots for each wavelength are done with the help of the electronic shutters.

Fig. 2.
Fig. 2.

Image composition in CDLHM. Figs. 2(a)–2(c) show the reconstructed red (671 nm), green (532 nm), and blue (473 nm) channels. Fig. 2(d) shows the full-color reconstructed image obtained by merging Figs. 2(a)–2(c). The microscope was set up to NA=0.32 (L=10.1mm).

Fig. 3.
Fig. 3.

Micrometer resolution with CDLHM. (a) The full-color amplitude reconstruction of a section of the head of a fruit fly imaged with CDLHM at NA=0.37 (L=8.5mm). The reddish ommatidia are clearly visible. Solid/dotted curves in (b) represent the profile along the white line in the circle/square of (a). The squared area is zoomed in in the upper right corner of (a).

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

I˜(r)=|Uscat(r)|2+[Uscat(r)Uref*(r)+Uscat*(r)Uref(r)].
Uscat(r)=ScreenI˜(r)exp[i2πλ(rr|r|)]dr,
Uscat(sΔx,tΔy,z)=ΔxΔy×exp[ik(s2ΔxΔx+t2ΔyΔy)/2L]×m=M/2(M/2)1n=N/2(N/2)1I˜(mΔx,nΔy)×exp[ik(m2ΔxΔx+n2ΔyΔy)/2L]×exp[ik((sm)2ΔxΔx+(tn)2ΔyΔy)/2L].

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