We developed an optical-fiber-based photomechanical gene transfer system for endoscopic or catheter-based application. A fiber tip with a laser-absorbing film covered with a transparent plastic disk for plasma confinement was attached to a quartz fiber; the film was irradiated with nanosecond laser pulses transmitted through the fiber to generate photomechanical waves (PMWs). Characteristics of PMWs emitted from the fiber tip were examined to confirm the necessary conditions for gene transfer. We then attempted to transfer reporter genes to the rat skin as a test tissue in vivo with the fiber system, and the results showed significantly high protein levels and spatially selective pinpoint gene expressions in the tissue.
© 2011 Optical Society of AmericaFull Article | PDF Article
Mitsuhiro Terakawa, Makoto Ogura, Shunichi Sato, Hitoshi Wakisaka, Hiroshi Ashida, Maki Uenoyama, Yoshinori Masaki, and Minoru Obara
Opt. Lett. 29(11) 1227-1229 (2004)
J. Baumgart, W. Bintig, A. Ngezahayo, S. Willenbrock, H. Murua Escobar, W. Ertmer, H. Lubatschowski, and A. Heisterkamp
Opt. Express 16(5) 3021-3031 (2008)
Aisada Uchugonova, Karsten König, Rainer Bueckle, Andreas Isemann, and Gabriel Tempea
Opt. Express 16(13) 9357-9364 (2008)