Abstract

We introduce and experimentally demonstrate a fast and accurate method for quantitative imaging of the dynamics of live biological cells. Using a dual-channel interferometric setup, two phase-shifted interferograms of nearly transparent biological samples are acquired in a single digital camera exposure and digitally processed into the phase profile of the sample. Since two interferograms of the same sample are acquired simultaneously, most of the common phase noise is eliminated, enabling the visualization of millisecond-scale dynamic biological phenomena with subnanometer optical path length temporal stability.

© 2009 Optical Society of America

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References

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2008

2007

K. J. Chalut, W. J. Brown, and A. Wax, Opt. Express 15, 3047 (2007).
[CrossRef] [PubMed]

Y. Zhu, L. Liu, Y. Zhi, Z. Luan, and D. Liu, Proc. SPIE 6671, 66711D (2007).
[CrossRef]

2005

2004

2003

1999

1998

Badizadegan, K.

Bevilacqua, F.

Brock, N.

N. Brock, J. Hayes, B. Kimbrough, J. Millerd, M. North-Morris, M. Novak, and J. Wyant, Proc. SPIE 5875, 58750F (2005).
[CrossRef]

Brown, W. J.

Chalut, K. J.

Colomb, T.

Cuche, E.

Dasari, R. R.

Deflores, L. P.

Depeursinge, C.

Emery, Y.

Feld, M. S.

Hayes, J.

N. Brock, J. Hayes, B. Kimbrough, J. Millerd, M. North-Morris, M. Novak, and J. Wyant, Proc. SPIE 5875, 58750F (2005).
[CrossRef]

Ikeda, T.

Iwai, H.

Kiire, T.

Kimbrough, B.

N. Brock, J. Hayes, B. Kimbrough, J. Millerd, M. North-Morris, M. Novak, and J. Wyant, Proc. SPIE 5875, 58750F (2005).
[CrossRef]

Liu, D.

Y. Zhu, L. Liu, Y. Zhi, Z. Luan, and D. Liu, Proc. SPIE 6671, 66711D (2007).
[CrossRef]

Liu, L.

Y. Zhu, L. Liu, Y. Zhi, Z. Luan, and D. Liu, Proc. SPIE 6671, 66711D (2007).
[CrossRef]

Luan, Z.

Y. Zhu, L. Liu, Y. Zhi, Z. Luan, and D. Liu, Proc. SPIE 6671, 66711D (2007).
[CrossRef]

Magistretti, P. J.

Marquet, P.

Meneses-Fabian, C.

Millerd, J.

N. Brock, J. Hayes, B. Kimbrough, J. Millerd, M. North-Morris, M. Novak, and J. Wyant, Proc. SPIE 5875, 58750F (2005).
[CrossRef]

Muñoz, J.

Nakadate, S.

North-Morris, M.

N. Brock, J. Hayes, B. Kimbrough, J. Millerd, M. North-Morris, M. Novak, and J. Wyant, Proc. SPIE 5875, 58750F (2005).
[CrossRef]

Novak, M.

N. Brock, J. Hayes, B. Kimbrough, J. Millerd, M. North-Morris, M. Novak, and J. Wyant, Proc. SPIE 5875, 58750F (2005).
[CrossRef]

Páez, G.

Pluta, M.

M. Pluta, Advanced Light Microscopy (Elsevier Science Publishing, 1988), Vol. 2.

Popescu, G.

Rappaz, B.

Rodriguez-Zurita, G.

Shibuya, M.

Strojnik, M.

Toto-Arellano, N.

Vaughan, J. C.

Vázquez-Castillo, J. F.

Wax, A.

Wyant, J.

N. Brock, J. Hayes, B. Kimbrough, J. Millerd, M. North-Morris, M. Novak, and J. Wyant, Proc. SPIE 5875, 58750F (2005).
[CrossRef]

Yamaguchi, I.

Zhang, T.

Zhi, Y.

Y. Zhu, L. Liu, Y. Zhi, Z. Luan, and D. Liu, Proc. SPIE 6671, 66711D (2007).
[CrossRef]

Zhu, Y.

Y. Zhu, L. Liu, Y. Zhi, Z. Luan, and D. Liu, Proc. SPIE 6671, 66711D (2007).
[CrossRef]

Supplementary Material (2)

» Media 1: AVI (3739 KB)     
» Media 2: AVI (5430 KB)     

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Figures (3)

Fig. 1
Fig. 1

DQPM system. Dual-channel single-exposure interferometer for obtaining phase profiles of live dynamic biological cells: (a) entire interferometer, (b) 4f image splitting system.

Fig. 2
Fig. 2

MDA-MB-468 human breast cancer cell. (a) Regular microscopic imaging through the system. (b) Two phase-shifted interferograms captured in a single camera exposure. (c) Final unwrapped phase profile (Media 1). (d) Surface plot of the phase profile shown in (c). (e) Temporal phase stability without the sample and with the sample in the three points marked in (c). Viewing this figure in color online is highly recommended.

Fig. 3
Fig. 3

Final unwrapped phase profile of a rat beating myocyte (Media 2). Viewing this figure in color online is highly recommended.

Equations (4)

Equations on this page are rendered with MathJax. Learn more.

I 1 = I R + I S + 2 I S I R [ cos ( φ OBJ + φ C ) ] ,
I 2 = I R + I S + 2 I S I R [ cos ( φ OBJ + φ C + α ) ] ,
F = exp ( j φ C ) 1 exp ( j α ) [ I 1 I 2 + j H T { I 1 I 2 } ] ,
φ OBJ = arctan { Im F Re F } ,

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