Abstract
Fourier transform second-harmonic generation (SHG) microscopy has been applied to quantitatively compare the information content between SHG images obtained from the forward and backward direction for three tissue types: porcine tendon, sclera, and ear cartilage. Both signal types yield consistent information on the preferred orientation of collagen fibers. For all specimens, the Fourier transform of the forward and backward SHG images produces several overlapping peaks in the magnitude spectrum at various depths into the tissues, indicating that some information present in the forward SHG images can be extracted from the backward SHG images. This study highlights the potential of backward SHG microscopy for medical diagnostics.
© 2009 Optical Society of America
Full Article | PDF ArticleMore Like This
Mayandi Sivaguru, Sushmitha Durgam, Raghu Ambekar, David Luedtke, Glenn Fried, Allison Stewart, and Kimani C. Toussaint
Opt. Express 18(24) 24983-24993 (2010)
Tung Yuen Lau, Raghu Ambekar, and Kimani C. Toussaint
Opt. Express 20(19) 21821-21832 (2012)
Raghu Ambekar Ramachandra Rao, Monal R. Mehta, and Kimani C. Toussaint
Opt. Express 17(17) 14534-14542 (2009)