Abstract

Diffraction images record angle-resolved distribution of scattered light from a particle excited by coherent light and can correlate highly with the 3D morphology of a particle. We present a jet-in-fluid design of flow chamber for acquisition of clear diffraction images in a laminar flow. Diffraction images of polystyrene spheres of different diameters were acquired and found to correlate highly with the calculated ones based on the Mie theory. Fast Fourier transform analysis indicated that the measured images can be used to extract sphere diameter values. These results demonstrate the significant potentials of high-throughput diffraction imaging flow cytometry for extracting 3D morphological features of cells.

© 2009 Optical Society of America

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    [CrossRef] [PubMed]
  2. T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
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  3. J. Neukammer, C. Gohlke, A. Hope, T. Wessel, and H. Rinneberg, Appl. Opt. 42, 6388 (2003).
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  4. X. T. Su, K. Singh, C. Capjack, J. Petracek, C. Backhouse, and W. Rozmus, J. Biomed. Opt. 13, 024024 (2008).
    [CrossRef] [PubMed]
  5. J. Q. Lu, P. Yang, and X. H. Hu, J. Biomed. Opt. 10, 024022 (2005).
    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef] [PubMed]
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    [CrossRef]

2009 (1)

K. M. Jacobs, L. V. Yang, J. Ding, A. E. Ekpenyong, R. Castellone, J. Q. Lu, and X. H. Hu, J. Biophotonics 2, 521 (2009).
[CrossRef] [PubMed]

2008 (1)

X. T. Su, K. Singh, C. Capjack, J. Petracek, C. Backhouse, and W. Rozmus, J. Biomed. Opt. 13, 024024 (2008).
[CrossRef] [PubMed]

2007 (1)

2006 (1)

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

2005 (2)

2004 (1)

T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
[CrossRef] [PubMed]

2003 (2)

X. Ma, J. Q. Lu, R. S. Brock, K. M. Jacobs, P. Yang, and X. H. Hu, Phys. Med. Biol. 48, 4165 (2003).
[CrossRef]

J. Neukammer, C. Gohlke, A. Hope, T. Wessel, and H. Rinneberg, Appl. Opt. 42, 6388 (2003).
[CrossRef] [PubMed]

1979 (1)

T. Lindmo and H. B. Steen, Biophys. J. 28, 33 (1979).
[CrossRef] [PubMed]

1953 (1)

P. J. Crosland-Taylor, Nature 171, 37 (1953).
[CrossRef] [PubMed]

Backhouse, C.

X. T. Su, K. Singh, C. Capjack, J. Petracek, C. Backhouse, and W. Rozmus, J. Biomed. Opt. 13, 024024 (2008).
[CrossRef] [PubMed]

Basiji, D. A.

T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
[CrossRef] [PubMed]

Brock, R. S.

M. A. Yurkin, A. G. Hoekstra, R. S. Brock, and J. Q. Lu, Opt. Express 15, 17902 (2007).
[CrossRef] [PubMed]

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

R. S. Brock, X. H. Hu, P. Yang, and J. Q. Lu, Opt. Express 13, 5279 (2005).
[CrossRef] [PubMed]

X. Ma, J. Q. Lu, R. S. Brock, K. M. Jacobs, P. Yang, and X. H. Hu, Phys. Med. Biol. 48, 4165 (2003).
[CrossRef]

Capjack, C.

X. T. Su, K. Singh, C. Capjack, J. Petracek, C. Backhouse, and W. Rozmus, J. Biomed. Opt. 13, 024024 (2008).
[CrossRef] [PubMed]

Castellone, R.

K. M. Jacobs, L. V. Yang, J. Ding, A. E. Ekpenyong, R. Castellone, J. Q. Lu, and X. H. Hu, J. Biophotonics 2, 521 (2009).
[CrossRef] [PubMed]

Crosland-Taylor, P. J.

P. J. Crosland-Taylor, Nature 171, 37 (1953).
[CrossRef] [PubMed]

Ding, J.

K. M. Jacobs, L. V. Yang, J. Ding, A. E. Ekpenyong, R. Castellone, J. Q. Lu, and X. H. Hu, J. Biophotonics 2, 521 (2009).
[CrossRef] [PubMed]

Ekpenyong, A. E.

K. M. Jacobs, L. V. Yang, J. Ding, A. E. Ekpenyong, R. Castellone, J. Q. Lu, and X. H. Hu, J. Biophotonics 2, 521 (2009).
[CrossRef] [PubMed]

George, T. C.

T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
[CrossRef] [PubMed]

Gohlke, C.

Hall, B. E.

T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
[CrossRef] [PubMed]

Hoekstra, A. G.

Hope, A.

Hu, X. H.

K. M. Jacobs, L. V. Yang, J. Ding, A. E. Ekpenyong, R. Castellone, J. Q. Lu, and X. H. Hu, J. Biophotonics 2, 521 (2009).
[CrossRef] [PubMed]

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

R. S. Brock, X. H. Hu, P. Yang, and J. Q. Lu, Opt. Express 13, 5279 (2005).
[CrossRef] [PubMed]

J. Q. Lu, P. Yang, and X. H. Hu, J. Biomed. Opt. 10, 024022 (2005).
[CrossRef] [PubMed]

X. Ma, J. Q. Lu, R. S. Brock, K. M. Jacobs, P. Yang, and X. H. Hu, Phys. Med. Biol. 48, 4165 (2003).
[CrossRef]

Jacobs, K. M.

K. M. Jacobs, L. V. Yang, J. Ding, A. E. Ekpenyong, R. Castellone, J. Q. Lu, and X. H. Hu, J. Biophotonics 2, 521 (2009).
[CrossRef] [PubMed]

X. Ma, J. Q. Lu, R. S. Brock, K. M. Jacobs, P. Yang, and X. H. Hu, Phys. Med. Biol. 48, 4165 (2003).
[CrossRef]

Lindmo, T.

T. Lindmo and H. B. Steen, Biophys. J. 28, 33 (1979).
[CrossRef] [PubMed]

Lu, J. Q.

K. M. Jacobs, L. V. Yang, J. Ding, A. E. Ekpenyong, R. Castellone, J. Q. Lu, and X. H. Hu, J. Biophotonics 2, 521 (2009).
[CrossRef] [PubMed]

M. A. Yurkin, A. G. Hoekstra, R. S. Brock, and J. Q. Lu, Opt. Express 15, 17902 (2007).
[CrossRef] [PubMed]

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

R. S. Brock, X. H. Hu, P. Yang, and J. Q. Lu, Opt. Express 13, 5279 (2005).
[CrossRef] [PubMed]

J. Q. Lu, P. Yang, and X. H. Hu, J. Biomed. Opt. 10, 024022 (2005).
[CrossRef] [PubMed]

X. Ma, J. Q. Lu, R. S. Brock, K. M. Jacobs, P. Yang, and X. H. Hu, Phys. Med. Biol. 48, 4165 (2003).
[CrossRef]

Lynch, D. H.

T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
[CrossRef] [PubMed]

Ma, X.

X. Ma, J. Q. Lu, R. S. Brock, K. M. Jacobs, P. Yang, and X. H. Hu, Phys. Med. Biol. 48, 4165 (2003).
[CrossRef]

Morrissey, P. J.

T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
[CrossRef] [PubMed]

Mourant, J. R.

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

Neukammer, J.

Ortyn, W. E.

T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
[CrossRef] [PubMed]

Perry, D. J.

T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
[CrossRef] [PubMed]

Petracek, J.

X. T. Su, K. Singh, C. Capjack, J. Petracek, C. Backhouse, and W. Rozmus, J. Biomed. Opt. 13, 024024 (2008).
[CrossRef] [PubMed]

Rinneberg, H.

Rozmus, W.

X. T. Su, K. Singh, C. Capjack, J. Petracek, C. Backhouse, and W. Rozmus, J. Biomed. Opt. 13, 024024 (2008).
[CrossRef] [PubMed]

Seo, M. J.

T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
[CrossRef] [PubMed]

Singh, K.

X. T. Su, K. Singh, C. Capjack, J. Petracek, C. Backhouse, and W. Rozmus, J. Biomed. Opt. 13, 024024 (2008).
[CrossRef] [PubMed]

Steen, H. B.

T. Lindmo and H. B. Steen, Biophys. J. 28, 33 (1979).
[CrossRef] [PubMed]

Su, X. T.

X. T. Su, K. Singh, C. Capjack, J. Petracek, C. Backhouse, and W. Rozmus, J. Biomed. Opt. 13, 024024 (2008).
[CrossRef] [PubMed]

Weidner, D. A.

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

Wessel, T.

Yang, L. V.

K. M. Jacobs, L. V. Yang, J. Ding, A. E. Ekpenyong, R. Castellone, J. Q. Lu, and X. H. Hu, J. Biophotonics 2, 521 (2009).
[CrossRef] [PubMed]

Yang, P.

J. Q. Lu, P. Yang, and X. H. Hu, J. Biomed. Opt. 10, 024022 (2005).
[CrossRef] [PubMed]

R. S. Brock, X. H. Hu, P. Yang, and J. Q. Lu, Opt. Express 13, 5279 (2005).
[CrossRef] [PubMed]

X. Ma, J. Q. Lu, R. S. Brock, K. M. Jacobs, P. Yang, and X. H. Hu, Phys. Med. Biol. 48, 4165 (2003).
[CrossRef]

Yurkin, M. A.

Zimmerman, C. A.

T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
[CrossRef] [PubMed]

Appl. Opt. (1)

Biophys. J. (1)

T. Lindmo and H. B. Steen, Biophys. J. 28, 33 (1979).
[CrossRef] [PubMed]

Cytometry (1)

T. C. George, D. A. Basiji, B. E. Hall, D. H. Lynch, W. E. Ortyn, D. J. Perry, M. J. Seo, C. A. Zimmerman, and P. J. Morrissey, Cytometry 59, 237 (2004).
[CrossRef] [PubMed]

J. Biomed. Opt. (2)

X. T. Su, K. Singh, C. Capjack, J. Petracek, C. Backhouse, and W. Rozmus, J. Biomed. Opt. 13, 024024 (2008).
[CrossRef] [PubMed]

J. Q. Lu, P. Yang, and X. H. Hu, J. Biomed. Opt. 10, 024022 (2005).
[CrossRef] [PubMed]

J. Biophotonics (1)

K. M. Jacobs, L. V. Yang, J. Ding, A. E. Ekpenyong, R. Castellone, J. Q. Lu, and X. H. Hu, J. Biophotonics 2, 521 (2009).
[CrossRef] [PubMed]

J. Quant. Spectrosc. Radiat. Transf. (1)

R. S. Brock, X. H. Hu, D. A. Weidner, J. R. Mourant, and J. Q. Lu, J. Quant. Spectrosc. Radiat. Transf. 102, 25 (2006).
[CrossRef]

Nature (1)

P. J. Crosland-Taylor, Nature 171, 37 (1953).
[CrossRef] [PubMed]

Opt. Express (2)

Phys. Med. Biol. (1)

X. Ma, J. Q. Lu, R. S. Brock, K. M. Jacobs, P. Yang, and X. H. Hu, Phys. Med. Biol. 48, 4165 (2003).
[CrossRef]

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Figures (3)

Fig. 1
Fig. 1

Schematic of the jet-in-fluid flow chamber (not to scale in the z direction). The short dashed curves indicate the interface between the sheath fluid and cuvette fluid, while the long dashed lines indicate the cone angle of light collection by the microscope objective.

Fig. 2
Fig. 2

Measured diffraction images of polystyrene sphere of 9.6 μm in nominal diameter with a laser beam of 532 nm in wavelength at different flow speeds of the core fluid: (a) 4.4 mm/s, (b) 6.8 mm/s, (c) 10 mm/s, (d) 14 mm/s. The field of view of these images at the focused position of x = 0 is 140 × 180 μm, and the images were acquired at x = 200 μ m .

Fig. 3
Fig. 3

Top and middle panels, measured diffraction images of polystyrene spheres with a laser beam of 532 nm in wavelength and different nominal diameters and flow speeds of (a) 5.2 μm at 13 mm/s, (b) 9.6 μm at 4.4 mm/s, and (c) 25 μm at 14 mm/s and calculated diffraction images based on the Mie theory for single spheres ( n s = 1.588 ) immersed in water ( n h = 1.334 ) with different diameters of (d) 5.2 μm, (e) 9.6 μm, and (f) 25 μm. Bottom panel, (g) fast Fourier transform power spectra of the measured diffraction images at f z = 600 ( 1 Δ ) with the dc component removed and the arrows indicating selected peaks. Inset, frequency shift of the selected peaks versus nominal sphere diameters.

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