Abstract

A novel fiber-optic-based method for simultaneous time- and wavelength-resolved fluorescence spectroscopy for the rapid diagnosis of diseased tissue is demonstrated. By combining multiple bandpass and dichroic filters (405/40, 460/50, and 550/50) with different lengths of optical fiber (1, 10, and 19m) acting as an optical delay this system enables the near real-time acquisition and characterization of time-resolved fluorescence spectra using a single detector and excitation input. The recording of multiple fluorescence response pulses at selected wavelengths can be completed in hundreds of nanoseconds, which provides the capability of a real-time characterization of biological systems.

© 2008 Optical Society of America

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References

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  1. D. S. Elson, N. Galletly, C. Talbot, J. Requejo-Isidro, J. McGinty, C. Dunsby, P. M. P. Lanigan, I. Munro, R. K. P. Benninger, P. de Beule, E. Auksorius, L. Hegyi, A. Sandison, A. Wallace, P. Soutter, M. A. A. Neil, J. Lever, G. W. Stamp, and P. M. W. French, in Reviews in Fluorescence, C.D.Geddes and J.R.Lakowicz, eds. (Springer, 2006), p. 1.
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    [CrossRef]
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    [CrossRef]
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2006 (1)

J. A. Jo, Q. Fang, T. Papaioannou, J. D. Baker, A. H. Dorafshar, T. Reil, J. H. Qiao, M. C. Fishbein, J. A. Freischlag, and L. Marcu, J. Biomed. Opt. 11, 021004 (2006).
[CrossRef] [PubMed]

2004 (3)

J. Requejo-Isidro, J. McGinty, I. Munro, D. S. Elson, N. P. Galletly, M. J. Lever, M. A. A. Neil, G. W. Stamp, P. M. W. French, P. A. Kellett, J. D. Hares, and A. K. L. Dymoke-Bradshaw, Opt. Lett. 29, 2249 (2004).
[CrossRef] [PubMed]

Q. Y. Fang, T. Papaioannou, J. A. Jo, R. Vaitha, K. Shastry, and L. Marcu, Rev. Sci. Instrum. 75, 151 (2004).
[CrossRef]

M. V. Rusalov, S. I. Druzhinin, and B. M. Uzhinov, J. Fluoresc. 14, 193 (2004).
[CrossRef] [PubMed]

2003 (3)

2001 (2)

J. D. Pitts and M. A. Mycek, Rev. Sci. Instrum. 72, 3061 (2001).
[CrossRef]

L. Marcu, M. C. Fishbein, J. M. I. Maarek, and W. S. Grundfest, Arterioscler. Thromb. 21, 1244 (2001).
[CrossRef]

1999 (1)

T. Glanzmann, J. P. Ballini, H. van den Bergh, and G. Wagnieres, Rev. Sci. Instrum. 70, 4067 (1999).
[CrossRef]

Appl. Opt. (1)

Arterioscler. Thromb. (1)

L. Marcu, M. C. Fishbein, J. M. I. Maarek, and W. S. Grundfest, Arterioscler. Thromb. 21, 1244 (2001).
[CrossRef]

J. Biomed. Opt. (1)

J. A. Jo, Q. Fang, T. Papaioannou, J. D. Baker, A. H. Dorafshar, T. Reil, J. H. Qiao, M. C. Fishbein, J. A. Freischlag, and L. Marcu, J. Biomed. Opt. 11, 021004 (2006).
[CrossRef] [PubMed]

J. Chem. Phys. (1)

H. Pal, S. Nad, and M. Kumbhakar, J. Chem. Phys. 119, 443 (2003).
[CrossRef]

J. Fluoresc. (1)

M. V. Rusalov, S. I. Druzhinin, and B. M. Uzhinov, J. Fluoresc. 14, 193 (2004).
[CrossRef] [PubMed]

Lasers Surg. Med. (1)

T. J. Pfefer, D. Y. Paithankar, J. M. Poneros, K. T. Schomacker, and N. S. Nishioka, Lasers Surg. Med. 32, 10 (2003).
[CrossRef] [PubMed]

Opt. Lett. (1)

Rev. Sci. Instrum. (3)

Q. Y. Fang, T. Papaioannou, J. A. Jo, R. Vaitha, K. Shastry, and L. Marcu, Rev. Sci. Instrum. 75, 151 (2004).
[CrossRef]

T. Glanzmann, J. P. Ballini, H. van den Bergh, and G. Wagnieres, Rev. Sci. Instrum. 70, 4067 (1999).
[CrossRef]

J. D. Pitts and M. A. Mycek, Rev. Sci. Instrum. 72, 3061 (2001).
[CrossRef]

Other (2)

D. S. Elson, N. Galletly, C. Talbot, J. Requejo-Isidro, J. McGinty, C. Dunsby, P. M. P. Lanigan, I. Munro, R. K. P. Benninger, P. de Beule, E. Auksorius, L. Hegyi, A. Sandison, A. Wallace, P. Soutter, M. A. A. Neil, J. Lever, G. W. Stamp, and P. M. W. French, in Reviews in Fluorescence, C.D.Geddes and J.R.Lakowicz, eds. (Springer, 2006), p. 1.

J. R. Lakowicz, Principle of Fluorescence Spectroscopy (Springer, 2006).
[CrossRef]

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Figures (3)

Fig. 1
Fig. 1

Schematic of the STWRFS system.

Fig. 2
Fig. 2

Trigger timing diagram of the STWRFS system.

Fig. 3
Fig. 3

STWRFS measurement: (a) FIRF of C1, C120, RhB, and 9CA retrieved from (b) fluorescence response pulses originally recorded from pure or mixed dye solutions. A neutral density filter of 0.8 or 0.6 was added in front of filter 2 for C-1, C-120, and 9CA to avoid the saturation of the photomultiplier (PMT) due to the intense emission peaks 460 nm . For the analysis of tissue autofluorescence the light collecting efficiency can be optimized by designing the proper bandwidth and attenuation in each channel to produce correctly scaled signals on the detector.

Tables (1)

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Table 1 Fluorescence Lifetimes of Standard Dyes and Their Mixed Solutions

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