Abstract

A time-resolved confocal fluorescence spectroscopy system is built to measure the fine structure and localized biochemistry of epithelial tissue. It is found that the autofluorescence excited at $405\mathrm{nm}$ is sensitive to the cellular metabolism and can be used to sense the metabolic status of epithelial tissue. The decay of autofluorescence excited at $405\mathrm{nm}$ can be accurately fitted with a dual-exponential function consisting of short lifetime $(0.4–0.6\mathrm{ns})$ and long lifetime $(3–4\mathrm{ns})$ components. The ratio of the amplitudes of the two components provides information on the fine structure of epithelial tissue. We demonstrate that the combined depth- and time-resolved measurements with single excitation can potentially provide accurate information for the diagnosis of tissue pathology.

© 2006 Optical Society of America

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