Abstract

We investigate theoretically and experimentally the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. On the example of two-photon fluorescence imaging, we show that using a suitable number of measurements, precise correction can be obtained for up to 2 radians rms aberrations without optimising the aberration modes used for correction. We also investigate the number of photons required for accurate correction when signal acquisition is shot-noise limited. We show that only 104 to 105 photons are required for complete correction so that the correction process can be implemented with limited extra-illumination and associated photoperturbation. Finally, we provide guidelines for implementing an optimal correction algorithm depending on the experimental conditions.

© 2012 OSA

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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef]
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2011 (3)

D. Débarre, T. Vieille, and E. Beaurepaire, “Simple characterisation of a deformable mirror inside a high numerical aperture microscope using phase diversity,” J. Microsc. 244, 136–143 (2011).
[CrossRef] [PubMed]

A. Thayil and M. J. Booth, “Self calibration of sensorless adaptive optical microscopes,” J. Eur. Opt. Soc. 6, 11045 (2011).

R. Aviles-Espinosa, J. Andilla, R. Porcar-Guezenec, O. E. Olarte, M. Nieto, X. Levecq, D. Artigas, and P. Loza-Alvarez, “Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy,” Biomed. Opt. Express 2, 3135–3149 (2011).
[CrossRef] [PubMed]

2009 (3)

2008 (1)

2007 (1)

2006 (1)

M. Rueckel, J. Mack-Bucher, and W. Denk, “Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing,” Proc. Nat. Acad. Sci. 103, 17137–17142 (2006).
[CrossRef] [PubMed]

2003 (1)

2002 (1)

M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, “Adaptive aberration correction in a confocal microscope,” Proc. Nat. Acad. Sci. 99, 5788–5792 (2002).
[CrossRef] [PubMed]

2000 (1)

Andilla, J.

Artigas, D.

Aviles-Espinosa, R.

Beaurepaire, E.

D. Débarre, T. Vieille, and E. Beaurepaire, “Simple characterisation of a deformable mirror inside a high numerical aperture microscope using phase diversity,” J. Microsc. 244, 136–143 (2011).
[CrossRef] [PubMed]

N. Olivier, D. Débarre, and E. Beaurepaire, “Dynamic aberration correction for multiharmonic microscopy”, Opt. Lett. 34, 3145–3147 (2009).
[CrossRef] [PubMed]

Betzig, E.

N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods 7, 141–147 (2009).
[CrossRef] [PubMed]

Booth, M. J.

Botcherby, E. J.

Burns, D.

Débarre, D.

Denk, W.

M. Rueckel, J. Mack-Bucher, and W. Denk, “Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing,” Proc. Nat. Acad. Sci. 103, 17137–17142 (2006).
[CrossRef] [PubMed]

Evans, C. L.

Freudiger, C. W.

Girkin, J. M.

Ji, N.

N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods 7, 141–147 (2009).
[CrossRef] [PubMed]

Juškaitis, R.

M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, “Adaptive aberration correction in a confocal microscope,” Proc. Nat. Acad. Sci. 99, 5788–5792 (2002).
[CrossRef] [PubMed]

Levecq, X.

Loza-Alvarez, P.

Mack-Bucher, J.

M. Rueckel, J. Mack-Bucher, and W. Denk, “Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing,” Proc. Nat. Acad. Sci. 103, 17137–17142 (2006).
[CrossRef] [PubMed]

Marsh, P. N.

Milkie, D. E.

N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods 7, 141–147 (2009).
[CrossRef] [PubMed]

Neil, M. A. A.

M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, “Adaptive aberration correction in a confocal microscope,” Proc. Nat. Acad. Sci. 99, 5788–5792 (2002).
[CrossRef] [PubMed]

M. A. A. Neil, M. J. Booth, and T. Wilson, “New modal wave-front sensor: a theoretical analysis,” J. Opt. Soc. Am. A 17, 1098–1107 (2000).
[CrossRef]

Nieto, M.

Olarte, O. E.

Olivier, N.

Poland, S. P.

Porcar-Guezenec, R.

Rueckel, M.

M. Rueckel, J. Mack-Bucher, and W. Denk, “Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing,” Proc. Nat. Acad. Sci. 103, 17137–17142 (2006).
[CrossRef] [PubMed]

Srinivas, S.

Thayil, A.

A. Thayil and M. J. Booth, “Self calibration of sensorless adaptive optical microscopes,” J. Eur. Opt. Soc. 6, 11045 (2011).

Vieille, T.

D. Débarre, T. Vieille, and E. Beaurepaire, “Simple characterisation of a deformable mirror inside a high numerical aperture microscope using phase diversity,” J. Microsc. 244, 136–143 (2011).
[CrossRef] [PubMed]

Watanabe, T.

Wilson, T.

Wright, A. J.

Xie, X. S.

Biomed. Opt. Express (1)

J. Eur. Opt. Soc. (1)

A. Thayil and M. J. Booth, “Self calibration of sensorless adaptive optical microscopes,” J. Eur. Opt. Soc. 6, 11045 (2011).

J. Microsc. (1)

D. Débarre, T. Vieille, and E. Beaurepaire, “Simple characterisation of a deformable mirror inside a high numerical aperture microscope using phase diversity,” J. Microsc. 244, 136–143 (2011).
[CrossRef] [PubMed]

J. Opt. Soc. Am. A (1)

Nat. Methods (1)

N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods 7, 141–147 (2009).
[CrossRef] [PubMed]

Opt. Express (3)

Opt. Lett. (2)

Proc. Nat. Acad. Sci. (2)

M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, “Adaptive aberration correction in a confocal microscope,” Proc. Nat. Acad. Sci. 99, 5788–5792 (2002).
[CrossRef] [PubMed]

M. Rueckel, J. Mack-Bucher, and W. Denk, “Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing,” Proc. Nat. Acad. Sci. 103, 17137–17142 (2006).
[CrossRef] [PubMed]

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