Abstract

In this paper we present trap profile measurements for HeLa cells in Optoelectronic Tweezers (OET) based on a data projector. The data projector is used as a light source to illuminate amorphous Si creating virtual electrodes which are used to trap particles through dielectrophoresis. We show that although the trap stiffness is typically greater at the edges of the optical spot it is possible to create a trap with constant trap stiffness by reducing the trap’s size until it is similar to the object being trapped. We have successfully created a trap for HeLa cells with a constant trap stiffness of 3×10-6 Nm-1 (capable of moving the cell up to 50μms-1) with a 12μm diameter trap. We also calculate the depth of the potential well that the cell will experience due to the trap and find that it to be 1.6×10-16J(4×104 kBT).

© 2009 Optical Society of America

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    [CrossRef]
  5. H. Hwang, Y. J. Choi, W. Choi, S. H. Kim, J. Jang, and J. K. Park, "Interactive manipulation of blood cells using a lens-integrated liquid crystal display based optoelectronic tweezers system," Electrophoresis 29, 1203-1212 (2008).
    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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2008 (4)

H. Hwang, Y. J. Choi, W. Choi, S. H. Kim, J. Jang, and J. K. Park, "Interactive manipulation of blood cells using a lens-integrated liquid crystal display based optoelectronic tweezers system," Electrophoresis 29, 1203-1212 (2008).
[CrossRef] [PubMed]

A. Jamshidi, P. J. Pauzauskie, P. J. Schuck, A. T. Ohta, P. Y. Chiou, J. Chou, P. D. Yang, and M. C. Wu, "Dynamic manipulation and separation of individual semiconducting and metallic nanowires," Nature Photonics 2, 85-89 (2008).
[CrossRef]

S. Grilli, and P. Ferraro, "Dielectrophoretic trapping of suspended particles by selective pyroelectric effect in lithium niobate crystals," Appl. Phys. Lett. 92, 3 (2008).
[CrossRef]

J. K. Valley, A. Jamshidi, A. T. Ohta, H. Hsan-Yin, and M. C. Wu, "Operational regimes and physics present in optoelectronic tweezers," J. Microelectromech. Syst. 17, 342-350 (2008).
[CrossRef] [PubMed]

2007 (5)

M. Hoeb, J. O. Radler, S. Klein, M. Stutzmann, and M. S. Brandt, "Light-induced dielectrophoretic manipulation of DNA," Biophys. J. 93, 1032-1038 (2007).
[CrossRef] [PubMed]

A. T. Ohta, A. Jamshidi, J. K. Valley, H.-Y. Hsu, and M. C. Wu, "Optically actuated thermocapillary movement of gas bubbles on an absorbing substrate," Appl. Phys. Lett. 91, 074103-074103 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, "Optically controlled cell discrimination and trapping using optoelectronic tweezers," IEEE J. Sel. Top. Quantum Electron. 13, 235-243 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, F. Yu, R. Sun, and M. C. Wu, "Dynamic Cell and Microparticle Control via Optoelectronic Tweezers," J. Microelectromech. Syst. 16, 491-499 (2007).
[CrossRef]

S. L. Neale, M. Mazilu, J. I. B. Wilson, K. Dholakia, and T. F. Krauss, "The resolution of optical traps created by light induced dielectrophoresis (LIDEP)," Opt. Express 15, 12619-12626 (2007).
[CrossRef] [PubMed]

2005 (2)

Y.S. Lu, Y.P. Huang, J.A. Yeh, C. Lee and Y.H. Chang, "Controllability of non-contact manipulation by image dielectrophoresis" Opt. Quantum Electron. 37, 1385-1395 (2005).
[CrossRef]

P. Y. Chiou, A. T. Ohta, and M. C. Wu, "Massively parallel manipulation of single cells and microparticles using optical images," Nature 436, 370-372 (2005).
[CrossRef] [PubMed]

2003 (1)

A. R. Minerick, R. H. Zhou, P. Takhistov, and H. C. Chang, "Manipulation and characterization of red blood cells with alternating current fields in microdevices," Electrophoresis 24, 3703-3717 (2003).
[CrossRef] [PubMed]

2002 (1)

C. F. Chou, J. O. Tegenfeldt, O. Bakajin, S. S. Chan, E. C. Cox, N. Darnton, T. Duke, and R. H. Austin, "Electrodeless dielectrophoresis of single- and double-stranded DNA," Biophys. J. 83, 2170-2179 (2002).
[CrossRef] [PubMed]

1999 (1)

M. Hollinshead, A. Vanderplasschen, G. L. Smith, and D. J. Vaux, "Vaccinia virus intracellular mature virions contain only one lipid membrane," J. Virol. 73, 1503-1517 (1999).
[PubMed]

Austin, R. H.

C. F. Chou, J. O. Tegenfeldt, O. Bakajin, S. S. Chan, E. C. Cox, N. Darnton, T. Duke, and R. H. Austin, "Electrodeless dielectrophoresis of single- and double-stranded DNA," Biophys. J. 83, 2170-2179 (2002).
[CrossRef] [PubMed]

Bakajin, O.

C. F. Chou, J. O. Tegenfeldt, O. Bakajin, S. S. Chan, E. C. Cox, N. Darnton, T. Duke, and R. H. Austin, "Electrodeless dielectrophoresis of single- and double-stranded DNA," Biophys. J. 83, 2170-2179 (2002).
[CrossRef] [PubMed]

Bhardwaj, U.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, F. Yu, R. Sun, and M. C. Wu, "Dynamic Cell and Microparticle Control via Optoelectronic Tweezers," J. Microelectromech. Syst. 16, 491-499 (2007).
[CrossRef]

Brandt, M. S.

M. Hoeb, J. O. Radler, S. Klein, M. Stutzmann, and M. S. Brandt, "Light-induced dielectrophoretic manipulation of DNA," Biophys. J. 93, 1032-1038 (2007).
[CrossRef] [PubMed]

Chan, S. S.

C. F. Chou, J. O. Tegenfeldt, O. Bakajin, S. S. Chan, E. C. Cox, N. Darnton, T. Duke, and R. H. Austin, "Electrodeless dielectrophoresis of single- and double-stranded DNA," Biophys. J. 83, 2170-2179 (2002).
[CrossRef] [PubMed]

Chang, H. C.

A. R. Minerick, R. H. Zhou, P. Takhistov, and H. C. Chang, "Manipulation and characterization of red blood cells with alternating current fields in microdevices," Electrophoresis 24, 3703-3717 (2003).
[CrossRef] [PubMed]

Chang, Y.H.

Y.S. Lu, Y.P. Huang, J.A. Yeh, C. Lee and Y.H. Chang, "Controllability of non-contact manipulation by image dielectrophoresis" Opt. Quantum Electron. 37, 1385-1395 (2005).
[CrossRef]

Chiou, P. Y.

A. Jamshidi, P. J. Pauzauskie, P. J. Schuck, A. T. Ohta, P. Y. Chiou, J. Chou, P. D. Yang, and M. C. Wu, "Dynamic manipulation and separation of individual semiconducting and metallic nanowires," Nature Photonics 2, 85-89 (2008).
[CrossRef]

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, F. Yu, R. Sun, and M. C. Wu, "Dynamic Cell and Microparticle Control via Optoelectronic Tweezers," J. Microelectromech. Syst. 16, 491-499 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, "Optically controlled cell discrimination and trapping using optoelectronic tweezers," IEEE J. Sel. Top. Quantum Electron. 13, 235-243 (2007).
[CrossRef]

P. Y. Chiou, A. T. Ohta, and M. C. Wu, "Massively parallel manipulation of single cells and microparticles using optical images," Nature 436, 370-372 (2005).
[CrossRef] [PubMed]

Choi, W.

H. Hwang, Y. J. Choi, W. Choi, S. H. Kim, J. Jang, and J. K. Park, "Interactive manipulation of blood cells using a lens-integrated liquid crystal display based optoelectronic tweezers system," Electrophoresis 29, 1203-1212 (2008).
[CrossRef] [PubMed]

Choi, Y. J.

H. Hwang, Y. J. Choi, W. Choi, S. H. Kim, J. Jang, and J. K. Park, "Interactive manipulation of blood cells using a lens-integrated liquid crystal display based optoelectronic tweezers system," Electrophoresis 29, 1203-1212 (2008).
[CrossRef] [PubMed]

Chou, C. F.

C. F. Chou, J. O. Tegenfeldt, O. Bakajin, S. S. Chan, E. C. Cox, N. Darnton, T. Duke, and R. H. Austin, "Electrodeless dielectrophoresis of single- and double-stranded DNA," Biophys. J. 83, 2170-2179 (2002).
[CrossRef] [PubMed]

Chou, J.

A. Jamshidi, P. J. Pauzauskie, P. J. Schuck, A. T. Ohta, P. Y. Chiou, J. Chou, P. D. Yang, and M. C. Wu, "Dynamic manipulation and separation of individual semiconducting and metallic nanowires," Nature Photonics 2, 85-89 (2008).
[CrossRef]

Cox, E. C.

C. F. Chou, J. O. Tegenfeldt, O. Bakajin, S. S. Chan, E. C. Cox, N. Darnton, T. Duke, and R. H. Austin, "Electrodeless dielectrophoresis of single- and double-stranded DNA," Biophys. J. 83, 2170-2179 (2002).
[CrossRef] [PubMed]

Darnton, N.

C. F. Chou, J. O. Tegenfeldt, O. Bakajin, S. S. Chan, E. C. Cox, N. Darnton, T. Duke, and R. H. Austin, "Electrodeless dielectrophoresis of single- and double-stranded DNA," Biophys. J. 83, 2170-2179 (2002).
[CrossRef] [PubMed]

Dholakia, K.

Duke, T.

C. F. Chou, J. O. Tegenfeldt, O. Bakajin, S. S. Chan, E. C. Cox, N. Darnton, T. Duke, and R. H. Austin, "Electrodeless dielectrophoresis of single- and double-stranded DNA," Biophys. J. 83, 2170-2179 (2002).
[CrossRef] [PubMed]

Ferraro, P.

S. Grilli, and P. Ferraro, "Dielectrophoretic trapping of suspended particles by selective pyroelectric effect in lithium niobate crystals," Appl. Phys. Lett. 92, 3 (2008).
[CrossRef]

Grilli, S.

S. Grilli, and P. Ferraro, "Dielectrophoretic trapping of suspended particles by selective pyroelectric effect in lithium niobate crystals," Appl. Phys. Lett. 92, 3 (2008).
[CrossRef]

Han, T. H.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, F. Yu, R. Sun, and M. C. Wu, "Dynamic Cell and Microparticle Control via Optoelectronic Tweezers," J. Microelectromech. Syst. 16, 491-499 (2007).
[CrossRef]

Hoeb, M.

M. Hoeb, J. O. Radler, S. Klein, M. Stutzmann, and M. S. Brandt, "Light-induced dielectrophoretic manipulation of DNA," Biophys. J. 93, 1032-1038 (2007).
[CrossRef] [PubMed]

Hollinshead, M.

M. Hollinshead, A. Vanderplasschen, G. L. Smith, and D. J. Vaux, "Vaccinia virus intracellular mature virions contain only one lipid membrane," J. Virol. 73, 1503-1517 (1999).
[PubMed]

Hsan-Yin, H.

J. K. Valley, A. Jamshidi, A. T. Ohta, H. Hsan-Yin, and M. C. Wu, "Operational regimes and physics present in optoelectronic tweezers," J. Microelectromech. Syst. 17, 342-350 (2008).
[CrossRef] [PubMed]

Hsu, H. Y.

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, "Optically controlled cell discrimination and trapping using optoelectronic tweezers," IEEE J. Sel. Top. Quantum Electron. 13, 235-243 (2007).
[CrossRef]

Hsu, H.-Y.

A. T. Ohta, A. Jamshidi, J. K. Valley, H.-Y. Hsu, and M. C. Wu, "Optically actuated thermocapillary movement of gas bubbles on an absorbing substrate," Appl. Phys. Lett. 91, 074103-074103 (2007).
[CrossRef]

Huang, Y.P.

Y.S. Lu, Y.P. Huang, J.A. Yeh, C. Lee and Y.H. Chang, "Controllability of non-contact manipulation by image dielectrophoresis" Opt. Quantum Electron. 37, 1385-1395 (2005).
[CrossRef]

Hwang, H.

H. Hwang, Y. J. Choi, W. Choi, S. H. Kim, J. Jang, and J. K. Park, "Interactive manipulation of blood cells using a lens-integrated liquid crystal display based optoelectronic tweezers system," Electrophoresis 29, 1203-1212 (2008).
[CrossRef] [PubMed]

Jamshidi, A.

J. K. Valley, A. Jamshidi, A. T. Ohta, H. Hsan-Yin, and M. C. Wu, "Operational regimes and physics present in optoelectronic tweezers," J. Microelectromech. Syst. 17, 342-350 (2008).
[CrossRef] [PubMed]

A. Jamshidi, P. J. Pauzauskie, P. J. Schuck, A. T. Ohta, P. Y. Chiou, J. Chou, P. D. Yang, and M. C. Wu, "Dynamic manipulation and separation of individual semiconducting and metallic nanowires," Nature Photonics 2, 85-89 (2008).
[CrossRef]

A. T. Ohta, A. Jamshidi, J. K. Valley, H.-Y. Hsu, and M. C. Wu, "Optically actuated thermocapillary movement of gas bubbles on an absorbing substrate," Appl. Phys. Lett. 91, 074103-074103 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, "Optically controlled cell discrimination and trapping using optoelectronic tweezers," IEEE J. Sel. Top. Quantum Electron. 13, 235-243 (2007).
[CrossRef]

Jang, J.

H. Hwang, Y. J. Choi, W. Choi, S. H. Kim, J. Jang, and J. K. Park, "Interactive manipulation of blood cells using a lens-integrated liquid crystal display based optoelectronic tweezers system," Electrophoresis 29, 1203-1212 (2008).
[CrossRef] [PubMed]

Kim, S. H.

H. Hwang, Y. J. Choi, W. Choi, S. H. Kim, J. Jang, and J. K. Park, "Interactive manipulation of blood cells using a lens-integrated liquid crystal display based optoelectronic tweezers system," Electrophoresis 29, 1203-1212 (2008).
[CrossRef] [PubMed]

Klein, S.

M. Hoeb, J. O. Radler, S. Klein, M. Stutzmann, and M. S. Brandt, "Light-induced dielectrophoretic manipulation of DNA," Biophys. J. 93, 1032-1038 (2007).
[CrossRef] [PubMed]

Krauss, T. F.

Lau, A. N. K.

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, "Optically controlled cell discrimination and trapping using optoelectronic tweezers," IEEE J. Sel. Top. Quantum Electron. 13, 235-243 (2007).
[CrossRef]

Lee, C.

Y.S. Lu, Y.P. Huang, J.A. Yeh, C. Lee and Y.H. Chang, "Controllability of non-contact manipulation by image dielectrophoresis" Opt. Quantum Electron. 37, 1385-1395 (2005).
[CrossRef]

Liao, J. C.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, F. Yu, R. Sun, and M. C. Wu, "Dynamic Cell and Microparticle Control via Optoelectronic Tweezers," J. Microelectromech. Syst. 16, 491-499 (2007).
[CrossRef]

Lu, Y.S.

Y.S. Lu, Y.P. Huang, J.A. Yeh, C. Lee and Y.H. Chang, "Controllability of non-contact manipulation by image dielectrophoresis" Opt. Quantum Electron. 37, 1385-1395 (2005).
[CrossRef]

Mazilu, M.

McCabe, E. R. B.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, F. Yu, R. Sun, and M. C. Wu, "Dynamic Cell and Microparticle Control via Optoelectronic Tweezers," J. Microelectromech. Syst. 16, 491-499 (2007).
[CrossRef]

Minerick, A. R.

A. R. Minerick, R. H. Zhou, P. Takhistov, and H. C. Chang, "Manipulation and characterization of red blood cells with alternating current fields in microdevices," Electrophoresis 24, 3703-3717 (2003).
[CrossRef] [PubMed]

Neale, S. L.

Ohta, A. T.

J. K. Valley, A. Jamshidi, A. T. Ohta, H. Hsan-Yin, and M. C. Wu, "Operational regimes and physics present in optoelectronic tweezers," J. Microelectromech. Syst. 17, 342-350 (2008).
[CrossRef] [PubMed]

A. Jamshidi, P. J. Pauzauskie, P. J. Schuck, A. T. Ohta, P. Y. Chiou, J. Chou, P. D. Yang, and M. C. Wu, "Dynamic manipulation and separation of individual semiconducting and metallic nanowires," Nature Photonics 2, 85-89 (2008).
[CrossRef]

A. T. Ohta, A. Jamshidi, J. K. Valley, H.-Y. Hsu, and M. C. Wu, "Optically actuated thermocapillary movement of gas bubbles on an absorbing substrate," Appl. Phys. Lett. 91, 074103-074103 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, F. Yu, R. Sun, and M. C. Wu, "Dynamic Cell and Microparticle Control via Optoelectronic Tweezers," J. Microelectromech. Syst. 16, 491-499 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, "Optically controlled cell discrimination and trapping using optoelectronic tweezers," IEEE J. Sel. Top. Quantum Electron. 13, 235-243 (2007).
[CrossRef]

P. Y. Chiou, A. T. Ohta, and M. C. Wu, "Massively parallel manipulation of single cells and microparticles using optical images," Nature 436, 370-372 (2005).
[CrossRef] [PubMed]

Park, J. K.

H. Hwang, Y. J. Choi, W. Choi, S. H. Kim, J. Jang, and J. K. Park, "Interactive manipulation of blood cells using a lens-integrated liquid crystal display based optoelectronic tweezers system," Electrophoresis 29, 1203-1212 (2008).
[CrossRef] [PubMed]

Pauzauskie, P. J.

A. Jamshidi, P. J. Pauzauskie, P. J. Schuck, A. T. Ohta, P. Y. Chiou, J. Chou, P. D. Yang, and M. C. Wu, "Dynamic manipulation and separation of individual semiconducting and metallic nanowires," Nature Photonics 2, 85-89 (2008).
[CrossRef]

Phan, H. L.

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, "Optically controlled cell discrimination and trapping using optoelectronic tweezers," IEEE J. Sel. Top. Quantum Electron. 13, 235-243 (2007).
[CrossRef]

Radler, J. O.

M. Hoeb, J. O. Radler, S. Klein, M. Stutzmann, and M. S. Brandt, "Light-induced dielectrophoretic manipulation of DNA," Biophys. J. 93, 1032-1038 (2007).
[CrossRef] [PubMed]

Schuck, P. J.

A. Jamshidi, P. J. Pauzauskie, P. J. Schuck, A. T. Ohta, P. Y. Chiou, J. Chou, P. D. Yang, and M. C. Wu, "Dynamic manipulation and separation of individual semiconducting and metallic nanowires," Nature Photonics 2, 85-89 (2008).
[CrossRef]

Sherwood, S. W.

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, "Optically controlled cell discrimination and trapping using optoelectronic tweezers," IEEE J. Sel. Top. Quantum Electron. 13, 235-243 (2007).
[CrossRef]

Smith, G. L.

M. Hollinshead, A. Vanderplasschen, G. L. Smith, and D. J. Vaux, "Vaccinia virus intracellular mature virions contain only one lipid membrane," J. Virol. 73, 1503-1517 (1999).
[PubMed]

Stutzmann, M.

M. Hoeb, J. O. Radler, S. Klein, M. Stutzmann, and M. S. Brandt, "Light-induced dielectrophoretic manipulation of DNA," Biophys. J. 93, 1032-1038 (2007).
[CrossRef] [PubMed]

Sun, R.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, F. Yu, R. Sun, and M. C. Wu, "Dynamic Cell and Microparticle Control via Optoelectronic Tweezers," J. Microelectromech. Syst. 16, 491-499 (2007).
[CrossRef]

Takhistov, P.

A. R. Minerick, R. H. Zhou, P. Takhistov, and H. C. Chang, "Manipulation and characterization of red blood cells with alternating current fields in microdevices," Electrophoresis 24, 3703-3717 (2003).
[CrossRef] [PubMed]

Tegenfeldt, J. O.

C. F. Chou, J. O. Tegenfeldt, O. Bakajin, S. S. Chan, E. C. Cox, N. Darnton, T. Duke, and R. H. Austin, "Electrodeless dielectrophoresis of single- and double-stranded DNA," Biophys. J. 83, 2170-2179 (2002).
[CrossRef] [PubMed]

Valley, J. K.

J. K. Valley, A. Jamshidi, A. T. Ohta, H. Hsan-Yin, and M. C. Wu, "Operational regimes and physics present in optoelectronic tweezers," J. Microelectromech. Syst. 17, 342-350 (2008).
[CrossRef] [PubMed]

A. T. Ohta, A. Jamshidi, J. K. Valley, H.-Y. Hsu, and M. C. Wu, "Optically actuated thermocapillary movement of gas bubbles on an absorbing substrate," Appl. Phys. Lett. 91, 074103-074103 (2007).
[CrossRef]

Vanderplasschen, A.

M. Hollinshead, A. Vanderplasschen, G. L. Smith, and D. J. Vaux, "Vaccinia virus intracellular mature virions contain only one lipid membrane," J. Virol. 73, 1503-1517 (1999).
[PubMed]

Vaux, D. J.

M. Hollinshead, A. Vanderplasschen, G. L. Smith, and D. J. Vaux, "Vaccinia virus intracellular mature virions contain only one lipid membrane," J. Virol. 73, 1503-1517 (1999).
[PubMed]

Wilson, J. I. B.

Wu, M. C.

J. K. Valley, A. Jamshidi, A. T. Ohta, H. Hsan-Yin, and M. C. Wu, "Operational regimes and physics present in optoelectronic tweezers," J. Microelectromech. Syst. 17, 342-350 (2008).
[CrossRef] [PubMed]

A. Jamshidi, P. J. Pauzauskie, P. J. Schuck, A. T. Ohta, P. Y. Chiou, J. Chou, P. D. Yang, and M. C. Wu, "Dynamic manipulation and separation of individual semiconducting and metallic nanowires," Nature Photonics 2, 85-89 (2008).
[CrossRef]

A. T. Ohta, A. Jamshidi, J. K. Valley, H.-Y. Hsu, and M. C. Wu, "Optically actuated thermocapillary movement of gas bubbles on an absorbing substrate," Appl. Phys. Lett. 91, 074103-074103 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, F. Yu, R. Sun, and M. C. Wu, "Dynamic Cell and Microparticle Control via Optoelectronic Tweezers," J. Microelectromech. Syst. 16, 491-499 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, "Optically controlled cell discrimination and trapping using optoelectronic tweezers," IEEE J. Sel. Top. Quantum Electron. 13, 235-243 (2007).
[CrossRef]

P. Y. Chiou, A. T. Ohta, and M. C. Wu, "Massively parallel manipulation of single cells and microparticles using optical images," Nature 436, 370-372 (2005).
[CrossRef] [PubMed]

Yang, J. M.

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, "Optically controlled cell discrimination and trapping using optoelectronic tweezers," IEEE J. Sel. Top. Quantum Electron. 13, 235-243 (2007).
[CrossRef]

Yang, P. D.

A. Jamshidi, P. J. Pauzauskie, P. J. Schuck, A. T. Ohta, P. Y. Chiou, J. Chou, P. D. Yang, and M. C. Wu, "Dynamic manipulation and separation of individual semiconducting and metallic nanowires," Nature Photonics 2, 85-89 (2008).
[CrossRef]

Yeh, J.A.

Y.S. Lu, Y.P. Huang, J.A. Yeh, C. Lee and Y.H. Chang, "Controllability of non-contact manipulation by image dielectrophoresis" Opt. Quantum Electron. 37, 1385-1395 (2005).
[CrossRef]

Yu, F.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, F. Yu, R. Sun, and M. C. Wu, "Dynamic Cell and Microparticle Control via Optoelectronic Tweezers," J. Microelectromech. Syst. 16, 491-499 (2007).
[CrossRef]

Zhou, R. H.

A. R. Minerick, R. H. Zhou, P. Takhistov, and H. C. Chang, "Manipulation and characterization of red blood cells with alternating current fields in microdevices," Electrophoresis 24, 3703-3717 (2003).
[CrossRef] [PubMed]

Appl. Phys. Lett. (2)

A. T. Ohta, A. Jamshidi, J. K. Valley, H.-Y. Hsu, and M. C. Wu, "Optically actuated thermocapillary movement of gas bubbles on an absorbing substrate," Appl. Phys. Lett. 91, 074103-074103 (2007).
[CrossRef]

S. Grilli, and P. Ferraro, "Dielectrophoretic trapping of suspended particles by selective pyroelectric effect in lithium niobate crystals," Appl. Phys. Lett. 92, 3 (2008).
[CrossRef]

Biophys. J. (2)

M. Hoeb, J. O. Radler, S. Klein, M. Stutzmann, and M. S. Brandt, "Light-induced dielectrophoretic manipulation of DNA," Biophys. J. 93, 1032-1038 (2007).
[CrossRef] [PubMed]

C. F. Chou, J. O. Tegenfeldt, O. Bakajin, S. S. Chan, E. C. Cox, N. Darnton, T. Duke, and R. H. Austin, "Electrodeless dielectrophoresis of single- and double-stranded DNA," Biophys. J. 83, 2170-2179 (2002).
[CrossRef] [PubMed]

Electrophoresis (2)

A. R. Minerick, R. H. Zhou, P. Takhistov, and H. C. Chang, "Manipulation and characterization of red blood cells with alternating current fields in microdevices," Electrophoresis 24, 3703-3717 (2003).
[CrossRef] [PubMed]

H. Hwang, Y. J. Choi, W. Choi, S. H. Kim, J. Jang, and J. K. Park, "Interactive manipulation of blood cells using a lens-integrated liquid crystal display based optoelectronic tweezers system," Electrophoresis 29, 1203-1212 (2008).
[CrossRef] [PubMed]

IEEE J. Sel. Top. Quantum Electron. (1)

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, "Optically controlled cell discrimination and trapping using optoelectronic tweezers," IEEE J. Sel. Top. Quantum Electron. 13, 235-243 (2007).
[CrossRef]

J. Microelectromech. Syst. (2)

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, F. Yu, R. Sun, and M. C. Wu, "Dynamic Cell and Microparticle Control via Optoelectronic Tweezers," J. Microelectromech. Syst. 16, 491-499 (2007).
[CrossRef]

J. K. Valley, A. Jamshidi, A. T. Ohta, H. Hsan-Yin, and M. C. Wu, "Operational regimes and physics present in optoelectronic tweezers," J. Microelectromech. Syst. 17, 342-350 (2008).
[CrossRef] [PubMed]

J. Virol. (1)

M. Hollinshead, A. Vanderplasschen, G. L. Smith, and D. J. Vaux, "Vaccinia virus intracellular mature virions contain only one lipid membrane," J. Virol. 73, 1503-1517 (1999).
[PubMed]

Nature (1)

P. Y. Chiou, A. T. Ohta, and M. C. Wu, "Massively parallel manipulation of single cells and microparticles using optical images," Nature 436, 370-372 (2005).
[CrossRef] [PubMed]

Nature Photonics (1)

A. Jamshidi, P. J. Pauzauskie, P. J. Schuck, A. T. Ohta, P. Y. Chiou, J. Chou, P. D. Yang, and M. C. Wu, "Dynamic manipulation and separation of individual semiconducting and metallic nanowires," Nature Photonics 2, 85-89 (2008).
[CrossRef]

Opt. Express (1)

Opt. Quantum Electron. (1)

Y.S. Lu, Y.P. Huang, J.A. Yeh, C. Lee and Y.H. Chang, "Controllability of non-contact manipulation by image dielectrophoresis" Opt. Quantum Electron. 37, 1385-1395 (2005).
[CrossRef]

Other (3)

P. Y. Chiou, A. T. Ohta, and M. C. Wu, "Toward all optical lab-on-a-chip system: optical manipulation of both microfluid and microscopic particles," Proceedings of the SPIE, Volume 5514, 73-81 (2004).
[CrossRef]

C. Hsien-Chang, C. Chao-Hung, I. F. Cheng, and L. Chi-Chang, "Manipulation of Bioparticles on Electrodeless Dielectrophoretic Chip Based on AC Electrokinetic Control," in Nano/Micro Engineered and Molecular Systems, 2007. NEMS '07. 2nd IEEE International Conference on (2007), pp. 1175-1178.

M.P. Hughes, Nanoelectromechanics in engineering and biology, CRC Press (2003).

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Figures (6)

Fig. 1.
Fig. 1.

The OET device. Schematic showing the OET chamber consists of a top contact and a bottom photoconductive substrate with an illuminated region.

Fig. 2.
Fig. 2.

A trapped cell. Video frames from an experiment showing the light pattern, outlined in red, being used to trap the HeLa cell, outlined in white, at velocities A) and D) 10 μs-1, B) and E) 30 μms-1, C) and F) 50 μms-1. A) to C) show a spot diameter of 73μm, where the optical spot is large compared to the size of the cell it moves to the edge of the optical spot even at low velocities. D) to F) show a 12μm diameter spot, here the trap is similar size to the cell and it stays central at low velocities. X is the lateral distance between the center of the cell and the center of the trap, which are indicated by vertical lines.

Fig. 3.
Fig. 3.

Trap profile results. The experimental results are plotted as crosses and compared to numerical simulations plotted as solid lines for optical spot diameters 73μm (black), 49μm (red), 31μm (green), 12μm (blue).

Fig. 4.
Fig. 4.

Simulations of DEP. The experimentally measured crossover frequency is plotted as points along side simulated lines for a conductive core (radius 7.5μm, relative permittivity 50, and conductivity 0.53Sm-1) surrounded by a single shell of; A. Conductivity 0.87μSm-1, thickness varying from 20 to 1 nm from top to bottom curves. B. Thickness 5nm and conductivity varying from 0.1 to 3μSm-1 from top to bottom curves. The relative permittivity of the medium was assumed to be 78.

Fig. 5.
Fig. 5.

Measured light patterns and simulated forces. A) The profile of the optical spots are plotted as crosses and compared to saturated Gaussian curves plotted as solid lines for optical spot diameters 73μm (blue), 49μm (red), 31μm (green), 12μm (black). B) Simulated force profile at 1μm (green curve, reduced by a factor of 10), 3.5μm (red curve, reduced by a factor of 4) and 7.5μm (blue curve, reduced by a factor of 4/3) above the surface of the OET chamber. The experimental points for the 31μm trap are shown as crosses.

Fig. 6.
Fig. 6.

Potential energy well. The potential energy well experienced by the trapped HeLa cell is calculated from the force verses position profile for the 12 μm diameter trap. The depth of this potential energy well is 1.6×10-16 J.

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

F = 2 π r 3 ε m Re [ k ( ω ) ] E 2
k ( ω ) = ε p * ε m * ε p * + 2 ε m *
ε 1 eff * = ε 2 * ( r 2 r 1 ) 3 + 2 ε 1 * ε 2 * ε 1 * + 2 ε 2 * ( r 2 r 1 ) 3 ε 1 * ε 2 * ε 1 * + 2 ε 2 *

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