Abstract

We present a novel high-frequency photoacoustic microscopy system capable of imaging the microvasculature of living subjects in realtime to depths of a few mm. The system consists of a high-repetition-rate Q-switched pump laser, a tunable dye laser, a 30-MHz linear ultrasound array transducer, a multichannel high-frequency data acquisition system, and a shared-RAM multi-core-processor computer. Data acquisition, beamforming, scan conversion, and display are implemented in realtime at 50 frames per second. Clearly resolvable images of 6-μm-diameter carbon fibers are experimentally demonstrated at 80 μm separation distances. Realtime imaging performance is demonstrated on phantoms and in vivo with absorbing structures identified to depths of 2.5-3 mm. This work represents the first high-frequency realtime photoacoustic imaging system to our knowledge.

© 2008 Optical Society of America

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2008 (1)

M. Li, J. Oh, X. X., G. Ku, W. Wang, C. Li, G. Lungu, G. Stoica, and L. V. Wang, "Simultaneous molecular and hypoxia imaging of brain tumors in vivo using spectroscopic photoacoustic tomography," Proc. IEEE 96, 481-489 (2008).

2007 (3)

R. J. Zemp, R. Bitton, M. L. Li, K. K. Shung, G. Stoica, and L. V. Wang, "Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer," J. Biomed. Opt. 12, 010501 (2007).
[CrossRef]

M. Sivaramakrishnan, K. Maslov, H. F. Zhang, G. Stoica, and L. V. Wang, "Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels," Phys. Med. Biol 52, 1349-1361 (2007).
[CrossRef]

L. Li, R. J. Zemp, G. Lungu, G. Stoica, and L. V. Wang, "Photoacoustic imaging of lacZ gene expression in vivo," J. Biomed. Opt. 12, 020504 (2007).
[CrossRef]

2006 (7)

X. D. Wang, X. Y. Xie, G. N. Ku, and L. H. V. Wang, "Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography," J. Biomed. Opt. 11, 024015 (2006).
[CrossRef]

O. Couture, P. D. Bevan, E. Cherin, K. Cheung, P. N. Burns, and F. S. Foster, "Investigating perfluorohexane particles with high-frequency ultrasound," Ultrasound. Med. Biol. 32, 73-82 (2006).
[CrossRef]

M. H. Xu and L. H. V. Wang, "Photoacoustic imaging in biomedicine," Rev. Sci. Instrum. 77, 041101 (2006).

H. F. Zhang, K. Maslov, G. Stoica, and L. H. V. Wang, "Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging," Nat. Biotechnol. 24, 848-851 (2006).
[CrossRef]

J. M. Cannata, J. A. Williams, Q. F. Zhou, T. A. Ritter, and K. K. Shung, "Development of a 35-MHz piezo-composite ultrasound array for medical imaging," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 224-236 (2006).
[CrossRef]

C. H. Hu, X. C. Xu, J. M. Cannata, J. T. Yen, and K. K. Shung, "Development of a real-time, high-frequency ultrasound digital beamformer for high-frequency linear array transducers," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 317-323 (2006).
[CrossRef]

E. Zhang, and P. Beard, "Broadband ultrasound field mapping system using a wavelength tuned, optically scanned focused laser beam to address a Fabry Perot polymer film sensor," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 1330-1338 (2006).
[CrossRef]

2005 (4)

G. Ku, X. D. Wang, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Imaging of tumor angiogenesis in rat brains in vivo by photoacoustic tomography," Appl. Opt. 44, 770-775 (2005).
[CrossRef]

K. Maslov, G. Stoica, and L. V. H. Wang, "In vivo dark-field reflection-mode photoacoustic microscopy," Opt. Lett. 30, 625-627 (2005).
[CrossRef]

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, "Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo," IEEE Trans. Med. Imag. 24, 436-440 (2005).
[CrossRef]

C. Abbey, A. Borowsky, J. Gregg, E. McGoldrick, R. Cardiff, and S. Cherry, "Longitudinal correlations in a small-animal PET studies," Med. Phys. 32, 1901-1901 (2005).
[CrossRef]

2004 (1)

2003 (4)

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain," Nat. Biotechnol. 21, 803-806 (2003).
[CrossRef]

T. F. Massoud and S. S. Gambhir, "Molecular imaging in living subjects: seeing fundamental biological processes in a new light," Genes Dev. 17, 545-580 (2003).
[CrossRef]

R. A. Kruger, W. L. Kiser, D. R. Reinecke, and G. A. Kruger, "Thermoacoustic computed tomography using a conventional linear transducer array," Med. Phys. 30, 856-860 (2003).
[CrossRef]

R. Kruger, W. Kiser, D. Reinecke, and G. Kruger, "Molecular imaging with thermoacoustic computed tomography," Med. Phys. 30, 1542-1542 (2003).
[CrossRef]

2002 (1)

P. A. Dayton and K. W. Ferrara, "Targeted imaging using ultrasound," J. Magn. Res. 16, 362-377 (2002).
[CrossRef]

2000 (1)

1999 (2)

S. A. Telenkov, B. S. Tanenbaum, D. M. Goodman, J. S. Nelson, and T. E. Milner, "In vivo infrared tomographic imaging of laser-heated blood vessels," IEEE J. Sel. Top. Quantum Electron. 5, 1193-1199 (1999).
[CrossRef]

R. O. Esenaliev, A. A. Karabutov, and A. A. Oraevsky, "Sensitivity of laser opto-acoustic imaging in detection of small deeply embedded tumors," IEEE J. Select. Top. Quantum Electron. 5, 981-988 (1999).
[CrossRef]

1998 (1)

G. E. Moore, "Cramming More Components Onto Integrated Circuits," Proc. IEEE 86, 82-85 (1998).
[CrossRef]

1997 (1)

1996 (1)

J. S. Nelson, T. E. Milner, B. S. Tanenbaum, D. M. Goodman, and M. J. C. VanGemert, "Infra-red tomography of port-wine-stain blood vessels in human skin," Lasers Med. Sci 11, 199-204 (1996).
[CrossRef]

Abbey, C.

C. Abbey, A. Borowsky, J. Gregg, E. McGoldrick, R. Cardiff, and S. Cherry, "Longitudinal correlations in a small-animal PET studies," Med. Phys. 32, 1901-1901 (2005).
[CrossRef]

Beard, P.

E. Zhang, and P. Beard, "Broadband ultrasound field mapping system using a wavelength tuned, optically scanned focused laser beam to address a Fabry Perot polymer film sensor," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 1330-1338 (2006).
[CrossRef]

Bevan, P. D.

O. Couture, P. D. Bevan, E. Cherin, K. Cheung, P. N. Burns, and F. S. Foster, "Investigating perfluorohexane particles with high-frequency ultrasound," Ultrasound. Med. Biol. 32, 73-82 (2006).
[CrossRef]

Bitton, R.

R. J. Zemp, R. Bitton, M. L. Li, K. K. Shung, G. Stoica, and L. V. Wang, "Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer," J. Biomed. Opt. 12, 010501 (2007).
[CrossRef]

Boas, D.

Boas, D. A.

Borowsky, A.

C. Abbey, A. Borowsky, J. Gregg, E. McGoldrick, R. Cardiff, and S. Cherry, "Longitudinal correlations in a small-animal PET studies," Med. Phys. 32, 1901-1901 (2005).
[CrossRef]

Burns, P. N.

O. Couture, P. D. Bevan, E. Cherin, K. Cheung, P. N. Burns, and F. S. Foster, "Investigating perfluorohexane particles with high-frequency ultrasound," Ultrasound. Med. Biol. 32, 73-82 (2006).
[CrossRef]

Cannata, J. M.

J. M. Cannata, J. A. Williams, Q. F. Zhou, T. A. Ritter, and K. K. Shung, "Development of a 35-MHz piezo-composite ultrasound array for medical imaging," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 224-236 (2006).
[CrossRef]

C. H. Hu, X. C. Xu, J. M. Cannata, J. T. Yen, and K. K. Shung, "Development of a real-time, high-frequency ultrasound digital beamformer for high-frequency linear array transducers," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 317-323 (2006).
[CrossRef]

Cardiff, R.

C. Abbey, A. Borowsky, J. Gregg, E. McGoldrick, R. Cardiff, and S. Cherry, "Longitudinal correlations in a small-animal PET studies," Med. Phys. 32, 1901-1901 (2005).
[CrossRef]

Cherin, E.

O. Couture, P. D. Bevan, E. Cherin, K. Cheung, P. N. Burns, and F. S. Foster, "Investigating perfluorohexane particles with high-frequency ultrasound," Ultrasound. Med. Biol. 32, 73-82 (2006).
[CrossRef]

Cherry, S.

C. Abbey, A. Borowsky, J. Gregg, E. McGoldrick, R. Cardiff, and S. Cherry, "Longitudinal correlations in a small-animal PET studies," Med. Phys. 32, 1901-1901 (2005).
[CrossRef]

Cheung, K.

O. Couture, P. D. Bevan, E. Cherin, K. Cheung, P. N. Burns, and F. S. Foster, "Investigating perfluorohexane particles with high-frequency ultrasound," Ultrasound. Med. Biol. 32, 73-82 (2006).
[CrossRef]

Couture, O.

O. Couture, P. D. Bevan, E. Cherin, K. Cheung, P. N. Burns, and F. S. Foster, "Investigating perfluorohexane particles with high-frequency ultrasound," Ultrasound. Med. Biol. 32, 73-82 (2006).
[CrossRef]

Dale, A. M.

Dayton, P. A.

P. A. Dayton and K. W. Ferrara, "Targeted imaging using ultrasound," J. Magn. Res. 16, 362-377 (2002).
[CrossRef]

Dunn, A.

Dunn, A. K.

Esenaliev, R. O.

R. O. Esenaliev, A. A. Karabutov, and A. A. Oraevsky, "Sensitivity of laser opto-acoustic imaging in detection of small deeply embedded tumors," IEEE J. Select. Top. Quantum Electron. 5, 981-988 (1999).
[CrossRef]

Ferrara, K. W.

P. A. Dayton and K. W. Ferrara, "Targeted imaging using ultrasound," J. Magn. Res. 16, 362-377 (2002).
[CrossRef]

Foster, F. S.

O. Couture, P. D. Bevan, E. Cherin, K. Cheung, P. N. Burns, and F. S. Foster, "Investigating perfluorohexane particles with high-frequency ultrasound," Ultrasound. Med. Biol. 32, 73-82 (2006).
[CrossRef]

Frenz, M.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, "Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo," IEEE Trans. Med. Imag. 24, 436-440 (2005).
[CrossRef]

Gambhir, S. S.

T. F. Massoud and S. S. Gambhir, "Molecular imaging in living subjects: seeing fundamental biological processes in a new light," Genes Dev. 17, 545-580 (2003).
[CrossRef]

Goodman, D. M.

S. A. Telenkov, B. S. Tanenbaum, D. M. Goodman, J. S. Nelson, and T. E. Milner, "In vivo infrared tomographic imaging of laser-heated blood vessels," IEEE J. Sel. Top. Quantum Electron. 5, 1193-1199 (1999).
[CrossRef]

J. S. Nelson, T. E. Milner, B. S. Tanenbaum, D. M. Goodman, and M. J. C. VanGemert, "Infra-red tomography of port-wine-stain blood vessels in human skin," Lasers Med. Sci 11, 199-204 (1996).
[CrossRef]

Gregg, J.

C. Abbey, A. Borowsky, J. Gregg, E. McGoldrick, R. Cardiff, and S. Cherry, "Longitudinal correlations in a small-animal PET studies," Med. Phys. 32, 1901-1901 (2005).
[CrossRef]

Hillman, E. M. C.

Hu, C. H.

C. H. Hu, X. C. Xu, J. M. Cannata, J. T. Yen, and K. K. Shung, "Development of a real-time, high-frequency ultrasound digital beamformer for high-frequency linear array transducers," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 317-323 (2006).
[CrossRef]

Jacques, S. L.

Jaeger, M.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, "Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo," IEEE Trans. Med. Imag. 24, 436-440 (2005).
[CrossRef]

Karabutov, A. A.

R. O. Esenaliev, A. A. Karabutov, and A. A. Oraevsky, "Sensitivity of laser opto-acoustic imaging in detection of small deeply embedded tumors," IEEE J. Select. Top. Quantum Electron. 5, 981-988 (1999).
[CrossRef]

Kiser, W.

R. Kruger, W. Kiser, D. Reinecke, and G. Kruger, "Molecular imaging with thermoacoustic computed tomography," Med. Phys. 30, 1542-1542 (2003).
[CrossRef]

Kiser, W. L.

R. A. Kruger, W. L. Kiser, D. R. Reinecke, and G. A. Kruger, "Thermoacoustic computed tomography using a conventional linear transducer array," Med. Phys. 30, 856-860 (2003).
[CrossRef]

Kruger, G.

R. Kruger, W. Kiser, D. Reinecke, and G. Kruger, "Molecular imaging with thermoacoustic computed tomography," Med. Phys. 30, 1542-1542 (2003).
[CrossRef]

Kruger, G. A.

R. A. Kruger, W. L. Kiser, D. R. Reinecke, and G. A. Kruger, "Thermoacoustic computed tomography using a conventional linear transducer array," Med. Phys. 30, 856-860 (2003).
[CrossRef]

Kruger, R.

R. Kruger, W. Kiser, D. Reinecke, and G. Kruger, "Molecular imaging with thermoacoustic computed tomography," Med. Phys. 30, 1542-1542 (2003).
[CrossRef]

Kruger, R. A.

R. A. Kruger, W. L. Kiser, D. R. Reinecke, and G. A. Kruger, "Thermoacoustic computed tomography using a conventional linear transducer array," Med. Phys. 30, 856-860 (2003).
[CrossRef]

Ku, G.

G. Ku, X. D. Wang, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Imaging of tumor angiogenesis in rat brains in vivo by photoacoustic tomography," Appl. Opt. 44, 770-775 (2005).
[CrossRef]

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain," Nat. Biotechnol. 21, 803-806 (2003).
[CrossRef]

Ku, G. N.

X. D. Wang, X. Y. Xie, G. N. Ku, and L. H. V. Wang, "Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography," J. Biomed. Opt. 11, 024015 (2006).
[CrossRef]

Lemor, R.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, "Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo," IEEE Trans. Med. Imag. 24, 436-440 (2005).
[CrossRef]

Li, L.

L. Li, R. J. Zemp, G. Lungu, G. Stoica, and L. V. Wang, "Photoacoustic imaging of lacZ gene expression in vivo," J. Biomed. Opt. 12, 020504 (2007).
[CrossRef]

Li, M.

M. Li, J. Oh, X. X., G. Ku, W. Wang, C. Li, G. Lungu, G. Stoica, and L. V. Wang, "Simultaneous molecular and hypoxia imaging of brain tumors in vivo using spectroscopic photoacoustic tomography," Proc. IEEE 96, 481-489 (2008).

Li, M. L.

R. J. Zemp, R. Bitton, M. L. Li, K. K. Shung, G. Stoica, and L. V. Wang, "Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer," J. Biomed. Opt. 12, 010501 (2007).
[CrossRef]

Lungu, G.

L. Li, R. J. Zemp, G. Lungu, G. Stoica, and L. V. Wang, "Photoacoustic imaging of lacZ gene expression in vivo," J. Biomed. Opt. 12, 020504 (2007).
[CrossRef]

Maslov, K.

M. Sivaramakrishnan, K. Maslov, H. F. Zhang, G. Stoica, and L. V. Wang, "Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels," Phys. Med. Biol 52, 1349-1361 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. H. V. Wang, "Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging," Nat. Biotechnol. 24, 848-851 (2006).
[CrossRef]

K. Maslov, G. Stoica, and L. V. H. Wang, "In vivo dark-field reflection-mode photoacoustic microscopy," Opt. Lett. 30, 625-627 (2005).
[CrossRef]

Massoud, T. F.

T. F. Massoud and S. S. Gambhir, "Molecular imaging in living subjects: seeing fundamental biological processes in a new light," Genes Dev. 17, 545-580 (2003).
[CrossRef]

McGoldrick, E.

C. Abbey, A. Borowsky, J. Gregg, E. McGoldrick, R. Cardiff, and S. Cherry, "Longitudinal correlations in a small-animal PET studies," Med. Phys. 32, 1901-1901 (2005).
[CrossRef]

Milner, T. E.

S. A. Telenkov, B. S. Tanenbaum, D. M. Goodman, J. S. Nelson, and T. E. Milner, "In vivo infrared tomographic imaging of laser-heated blood vessels," IEEE J. Sel. Top. Quantum Electron. 5, 1193-1199 (1999).
[CrossRef]

J. S. Nelson, T. E. Milner, B. S. Tanenbaum, D. M. Goodman, and M. J. C. VanGemert, "Infra-red tomography of port-wine-stain blood vessels in human skin," Lasers Med. Sci 11, 199-204 (1996).
[CrossRef]

Moore, G. E.

G. E. Moore, "Cramming More Components Onto Integrated Circuits," Proc. IEEE 86, 82-85 (1998).
[CrossRef]

Nelson, J. S.

S. A. Telenkov, B. S. Tanenbaum, D. M. Goodman, J. S. Nelson, and T. E. Milner, "In vivo infrared tomographic imaging of laser-heated blood vessels," IEEE J. Sel. Top. Quantum Electron. 5, 1193-1199 (1999).
[CrossRef]

J. S. Nelson, T. E. Milner, B. S. Tanenbaum, D. M. Goodman, and M. J. C. VanGemert, "Infra-red tomography of port-wine-stain blood vessels in human skin," Lasers Med. Sci 11, 199-204 (1996).
[CrossRef]

Niederhauser, J. J.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, "Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo," IEEE Trans. Med. Imag. 24, 436-440 (2005).
[CrossRef]

Oh, J.

M. Li, J. Oh, X. X., G. Ku, W. Wang, C. Li, G. Lungu, G. Stoica, and L. V. Wang, "Simultaneous molecular and hypoxia imaging of brain tumors in vivo using spectroscopic photoacoustic tomography," Proc. IEEE 96, 481-489 (2008).

Oraevsky, A. A.

R. O. Esenaliev, A. A. Karabutov, and A. A. Oraevsky, "Sensitivity of laser opto-acoustic imaging in detection of small deeply embedded tumors," IEEE J. Select. Top. Quantum Electron. 5, 981-988 (1999).
[CrossRef]

A. A. Oraevsky, S. L. Jacques, and F. K. Tittel, "Measurement of tissue optical properties by time-resolved detection of laser-induced transient stress," Appl. Opt. 36, 402-415 (1997).
[CrossRef]

Pang, Y. J.

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain," Nat. Biotechnol. 21, 803-806 (2003).
[CrossRef]

Reinecke, D.

R. Kruger, W. Kiser, D. Reinecke, and G. Kruger, "Molecular imaging with thermoacoustic computed tomography," Med. Phys. 30, 1542-1542 (2003).
[CrossRef]

Reinecke, D. R.

R. A. Kruger, W. L. Kiser, D. R. Reinecke, and G. A. Kruger, "Thermoacoustic computed tomography using a conventional linear transducer array," Med. Phys. 30, 856-860 (2003).
[CrossRef]

Ritter, T. A.

J. M. Cannata, J. A. Williams, Q. F. Zhou, T. A. Ritter, and K. K. Shung, "Development of a 35-MHz piezo-composite ultrasound array for medical imaging," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 224-236 (2006).
[CrossRef]

Shung, K. K.

R. J. Zemp, R. Bitton, M. L. Li, K. K. Shung, G. Stoica, and L. V. Wang, "Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer," J. Biomed. Opt. 12, 010501 (2007).
[CrossRef]

C. H. Hu, X. C. Xu, J. M. Cannata, J. T. Yen, and K. K. Shung, "Development of a real-time, high-frequency ultrasound digital beamformer for high-frequency linear array transducers," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 317-323 (2006).
[CrossRef]

J. M. Cannata, J. A. Williams, Q. F. Zhou, T. A. Ritter, and K. K. Shung, "Development of a 35-MHz piezo-composite ultrasound array for medical imaging," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 224-236 (2006).
[CrossRef]

Sivaramakrishnan, M.

M. Sivaramakrishnan, K. Maslov, H. F. Zhang, G. Stoica, and L. V. Wang, "Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels," Phys. Med. Biol 52, 1349-1361 (2007).
[CrossRef]

Stoica, G.

M. Sivaramakrishnan, K. Maslov, H. F. Zhang, G. Stoica, and L. V. Wang, "Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels," Phys. Med. Biol 52, 1349-1361 (2007).
[CrossRef]

R. J. Zemp, R. Bitton, M. L. Li, K. K. Shung, G. Stoica, and L. V. Wang, "Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer," J. Biomed. Opt. 12, 010501 (2007).
[CrossRef]

L. Li, R. J. Zemp, G. Lungu, G. Stoica, and L. V. Wang, "Photoacoustic imaging of lacZ gene expression in vivo," J. Biomed. Opt. 12, 020504 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. H. V. Wang, "Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging," Nat. Biotechnol. 24, 848-851 (2006).
[CrossRef]

K. Maslov, G. Stoica, and L. V. H. Wang, "In vivo dark-field reflection-mode photoacoustic microscopy," Opt. Lett. 30, 625-627 (2005).
[CrossRef]

G. Ku, X. D. Wang, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Imaging of tumor angiogenesis in rat brains in vivo by photoacoustic tomography," Appl. Opt. 44, 770-775 (2005).
[CrossRef]

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain," Nat. Biotechnol. 21, 803-806 (2003).
[CrossRef]

Tanenbaum, B. S.

S. A. Telenkov, B. S. Tanenbaum, D. M. Goodman, J. S. Nelson, and T. E. Milner, "In vivo infrared tomographic imaging of laser-heated blood vessels," IEEE J. Sel. Top. Quantum Electron. 5, 1193-1199 (1999).
[CrossRef]

J. S. Nelson, T. E. Milner, B. S. Tanenbaum, D. M. Goodman, and M. J. C. VanGemert, "Infra-red tomography of port-wine-stain blood vessels in human skin," Lasers Med. Sci 11, 199-204 (1996).
[CrossRef]

Telenkov, S. A.

S. A. Telenkov, B. S. Tanenbaum, D. M. Goodman, J. S. Nelson, and T. E. Milner, "In vivo infrared tomographic imaging of laser-heated blood vessels," IEEE J. Sel. Top. Quantum Electron. 5, 1193-1199 (1999).
[CrossRef]

Tittel, F. K.

VanGemert, M. J. C.

J. S. Nelson, T. E. Milner, B. S. Tanenbaum, D. M. Goodman, and M. J. C. VanGemert, "Infra-red tomography of port-wine-stain blood vessels in human skin," Lasers Med. Sci 11, 199-204 (1996).
[CrossRef]

Wang, L. H. V.

H. F. Zhang, K. Maslov, G. Stoica, and L. H. V. Wang, "Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging," Nat. Biotechnol. 24, 848-851 (2006).
[CrossRef]

X. D. Wang, X. Y. Xie, G. N. Ku, and L. H. V. Wang, "Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography," J. Biomed. Opt. 11, 024015 (2006).
[CrossRef]

M. H. Xu and L. H. V. Wang, "Photoacoustic imaging in biomedicine," Rev. Sci. Instrum. 77, 041101 (2006).

G. Ku, X. D. Wang, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Imaging of tumor angiogenesis in rat brains in vivo by photoacoustic tomography," Appl. Opt. 44, 770-775 (2005).
[CrossRef]

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain," Nat. Biotechnol. 21, 803-806 (2003).
[CrossRef]

Wang, L. V.

R. J. Zemp, R. Bitton, M. L. Li, K. K. Shung, G. Stoica, and L. V. Wang, "Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer," J. Biomed. Opt. 12, 010501 (2007).
[CrossRef]

M. Sivaramakrishnan, K. Maslov, H. F. Zhang, G. Stoica, and L. V. Wang, "Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels," Phys. Med. Biol 52, 1349-1361 (2007).
[CrossRef]

L. Li, R. J. Zemp, G. Lungu, G. Stoica, and L. V. Wang, "Photoacoustic imaging of lacZ gene expression in vivo," J. Biomed. Opt. 12, 020504 (2007).
[CrossRef]

Wang, L. V. H.

Wang, X. D.

X. D. Wang, X. Y. Xie, G. N. Ku, and L. H. V. Wang, "Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography," J. Biomed. Opt. 11, 024015 (2006).
[CrossRef]

G. Ku, X. D. Wang, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Imaging of tumor angiogenesis in rat brains in vivo by photoacoustic tomography," Appl. Opt. 44, 770-775 (2005).
[CrossRef]

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain," Nat. Biotechnol. 21, 803-806 (2003).
[CrossRef]

Weber, P.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, "Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo," IEEE Trans. Med. Imag. 24, 436-440 (2005).
[CrossRef]

Williams, J. A.

J. M. Cannata, J. A. Williams, Q. F. Zhou, T. A. Ritter, and K. K. Shung, "Development of a 35-MHz piezo-composite ultrasound array for medical imaging," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 224-236 (2006).
[CrossRef]

Xie, X. Y.

X. D. Wang, X. Y. Xie, G. N. Ku, and L. H. V. Wang, "Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography," J. Biomed. Opt. 11, 024015 (2006).
[CrossRef]

G. Ku, X. D. Wang, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Imaging of tumor angiogenesis in rat brains in vivo by photoacoustic tomography," Appl. Opt. 44, 770-775 (2005).
[CrossRef]

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain," Nat. Biotechnol. 21, 803-806 (2003).
[CrossRef]

Xu, M. H.

M. H. Xu and L. H. V. Wang, "Photoacoustic imaging in biomedicine," Rev. Sci. Instrum. 77, 041101 (2006).

Xu, X. C.

C. H. Hu, X. C. Xu, J. M. Cannata, J. T. Yen, and K. K. Shung, "Development of a real-time, high-frequency ultrasound digital beamformer for high-frequency linear array transducers," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 317-323 (2006).
[CrossRef]

Yen, J. T.

C. H. Hu, X. C. Xu, J. M. Cannata, J. T. Yen, and K. K. Shung, "Development of a real-time, high-frequency ultrasound digital beamformer for high-frequency linear array transducers," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 317-323 (2006).
[CrossRef]

Zemp, R. J.

R. J. Zemp, R. Bitton, M. L. Li, K. K. Shung, G. Stoica, and L. V. Wang, "Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer," J. Biomed. Opt. 12, 010501 (2007).
[CrossRef]

L. Li, R. J. Zemp, G. Lungu, G. Stoica, and L. V. Wang, "Photoacoustic imaging of lacZ gene expression in vivo," J. Biomed. Opt. 12, 020504 (2007).
[CrossRef]

Zhang, E.

E. Zhang, and P. Beard, "Broadband ultrasound field mapping system using a wavelength tuned, optically scanned focused laser beam to address a Fabry Perot polymer film sensor," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 1330-1338 (2006).
[CrossRef]

Zhang, H. F.

M. Sivaramakrishnan, K. Maslov, H. F. Zhang, G. Stoica, and L. V. Wang, "Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels," Phys. Med. Biol 52, 1349-1361 (2007).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. H. V. Wang, "Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging," Nat. Biotechnol. 24, 848-851 (2006).
[CrossRef]

Zhou, Q. F.

J. M. Cannata, J. A. Williams, Q. F. Zhou, T. A. Ritter, and K. K. Shung, "Development of a 35-MHz piezo-composite ultrasound array for medical imaging," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 224-236 (2006).
[CrossRef]

Appl. Opt. (2)

Genes Dev. (1)

T. F. Massoud and S. S. Gambhir, "Molecular imaging in living subjects: seeing fundamental biological processes in a new light," Genes Dev. 17, 545-580 (2003).
[CrossRef]

IEEE J. Sel. Top. Quantum Electron. (1)

S. A. Telenkov, B. S. Tanenbaum, D. M. Goodman, J. S. Nelson, and T. E. Milner, "In vivo infrared tomographic imaging of laser-heated blood vessels," IEEE J. Sel. Top. Quantum Electron. 5, 1193-1199 (1999).
[CrossRef]

IEEE J. Select. Top. Quantum Electron. (1)

R. O. Esenaliev, A. A. Karabutov, and A. A. Oraevsky, "Sensitivity of laser opto-acoustic imaging in detection of small deeply embedded tumors," IEEE J. Select. Top. Quantum Electron. 5, 981-988 (1999).
[CrossRef]

IEEE Trans. Med. Imag. (1)

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, "Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo," IEEE Trans. Med. Imag. 24, 436-440 (2005).
[CrossRef]

IEEE Trans. Ultrason. Ferroelectr. Freq. Control (3)

E. Zhang, and P. Beard, "Broadband ultrasound field mapping system using a wavelength tuned, optically scanned focused laser beam to address a Fabry Perot polymer film sensor," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 1330-1338 (2006).
[CrossRef]

J. M. Cannata, J. A. Williams, Q. F. Zhou, T. A. Ritter, and K. K. Shung, "Development of a 35-MHz piezo-composite ultrasound array for medical imaging," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 224-236 (2006).
[CrossRef]

C. H. Hu, X. C. Xu, J. M. Cannata, J. T. Yen, and K. K. Shung, "Development of a real-time, high-frequency ultrasound digital beamformer for high-frequency linear array transducers," IEEE Trans. Ultrason. Ferroelectr. Freq. Control 53, 317-323 (2006).
[CrossRef]

J. Biomed. Opt. (3)

R. J. Zemp, R. Bitton, M. L. Li, K. K. Shung, G. Stoica, and L. V. Wang, "Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer," J. Biomed. Opt. 12, 010501 (2007).
[CrossRef]

X. D. Wang, X. Y. Xie, G. N. Ku, and L. H. V. Wang, "Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography," J. Biomed. Opt. 11, 024015 (2006).
[CrossRef]

L. Li, R. J. Zemp, G. Lungu, G. Stoica, and L. V. Wang, "Photoacoustic imaging of lacZ gene expression in vivo," J. Biomed. Opt. 12, 020504 (2007).
[CrossRef]

J. Magn. Res. (1)

P. A. Dayton and K. W. Ferrara, "Targeted imaging using ultrasound," J. Magn. Res. 16, 362-377 (2002).
[CrossRef]

Lasers Med. Sci. (1)

J. S. Nelson, T. E. Milner, B. S. Tanenbaum, D. M. Goodman, and M. J. C. VanGemert, "Infra-red tomography of port-wine-stain blood vessels in human skin," Lasers Med. Sci 11, 199-204 (1996).
[CrossRef]

Med. Phys. (3)

C. Abbey, A. Borowsky, J. Gregg, E. McGoldrick, R. Cardiff, and S. Cherry, "Longitudinal correlations in a small-animal PET studies," Med. Phys. 32, 1901-1901 (2005).
[CrossRef]

R. A. Kruger, W. L. Kiser, D. R. Reinecke, and G. A. Kruger, "Thermoacoustic computed tomography using a conventional linear transducer array," Med. Phys. 30, 856-860 (2003).
[CrossRef]

R. Kruger, W. Kiser, D. Reinecke, and G. Kruger, "Molecular imaging with thermoacoustic computed tomography," Med. Phys. 30, 1542-1542 (2003).
[CrossRef]

Nat. Biotechnol. (2)

H. F. Zhang, K. Maslov, G. Stoica, and L. H. V. Wang, "Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging," Nat. Biotechnol. 24, 848-851 (2006).
[CrossRef]

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, "Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain," Nat. Biotechnol. 21, 803-806 (2003).
[CrossRef]

Opt. Lett. (3)

Phys. Med. Biol (1)

M. Sivaramakrishnan, K. Maslov, H. F. Zhang, G. Stoica, and L. V. Wang, "Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels," Phys. Med. Biol 52, 1349-1361 (2007).
[CrossRef]

Proc. IEEE (2)

M. Li, J. Oh, X. X., G. Ku, W. Wang, C. Li, G. Lungu, G. Stoica, and L. V. Wang, "Simultaneous molecular and hypoxia imaging of brain tumors in vivo using spectroscopic photoacoustic tomography," Proc. IEEE 96, 481-489 (2008).

G. E. Moore, "Cramming More Components Onto Integrated Circuits," Proc. IEEE 86, 82-85 (1998).
[CrossRef]

Rev. Sci. Instrum. (1)

M. H. Xu and L. H. V. Wang, "Photoacoustic imaging in biomedicine," Rev. Sci. Instrum. 77, 041101 (2006).

Ultrasound. Med. Biol. (1)

O. Couture, P. D. Bevan, E. Cherin, K. Cheung, P. N. Burns, and F. S. Foster, "Investigating perfluorohexane particles with high-frequency ultrasound," Ultrasound. Med. Biol. 32, 73-82 (2006).
[CrossRef]

Other (3)

R. Bitton, R. Zemp, L. Meng-Lin, J. Yen, L. H. Wang, and K. K. Shung, "Photoacoustic Microscopy with a 30 MHz Array and Receive System," in IEEE Ultrasonics Symposium (IEEE, 2006), pp. 389-392.

K. Wall, and G. R. Lockwood, "Modern implementation of a realtime 3D beamformer and scan converter system," in Ultrasonics Symposium (2005), pp. 1400-1403.

D. Napolitano, C. Ching-Hua, G. W. McLaughlin, D. DeBusschere, L. Y. L. Mo, and J. Ting-Lan, "Zone-based B-mode imaging," in IEEE Ultrasonics Symposium, (IEEE, 2003), pp. 25-28.

Supplementary Material (4)

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