Mid-infrared attenuated total reflection (Mid-IR-ATR) spectra (4000-380 cm<sup>−1</sup>) of pericarp, germ, and endosperm sections from sound and <i>Fusarium</i>-damaged wheat kernels of cultivars Everest and Tomahawk were collected using a Fourier transform infrared (FT-IR) spectrometer. The differences in infrared absorption bands between sound and <i>Fusarium</i>-damaged kernels were examined. Absorption bands in which differences were identified were compared with the mid-IR-ATR absorption bands of deoxynivalenol (DON) and <i>Fusarium graminearum</i> hyphae. Marked differences in absorption patterns were observed between sound and <i>Fusarium</i>-damaged pericarp and germ spectra, whereas those differences were negligible in the endosperm spectra. <i>Fusarium</i>-damaged pericarp and germ spectra exhibited a shift in the peak position of the band near 1035 cm<sup>−1</sup> along with increased absorptions at 1160, 1203, 1313, and 1375 cm<sup>−1</sup>, likely due to the influence of DON and fungi in the <i>Fusarium-</i>damaged kernel tissue matrix. These results suggest that infrared spectroscopy can detect DON in the surface tissues of <i>Fusarium-</i>damaged wheat kernels.

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