Abstract

In this paper quantitative imaging of biological cells using high-resolution full-field optical coherence microscopy (FF-OCM) is reported. The FF-OCM was realized using a swept-source system, a Mirau interferometer, and a CCD camera (a two-dimensional detection unit). A Mirau-interferometric objective lens was used to generate the interferometric signal. The signal was analyzed by a Fourier analysis technique. Optically sectioned amplitude images and a quantitative phase map of biological cells such as onion skin and red blood cells (RBCs) are demonstrated. Further, the refractive index profile of the RBCs is also presented. For the 50× Mirau objective, the experimentally achieved axial and transverse resolution of the present system are 3.8 and 1.2μm, respectively. The CCD provides parallel detection and measures enface images without X, Y, Z mechanical scanning.

© 2011 Optical Society of America

Full Article  |  PDF Article
Related Articles
Common-path interferometer for frequency-domain optical coherence tomography

Andrei B. Vakhtin, Daniel J. Kane, William R. Wood, and Kristen A. Peterson
Appl. Opt. 42(34) 6953-6958 (2003)

Invariant resolution dynamic focus OCM based on liquid crystal lens

S. Murali, K. S. Lee, and J. P Rolland
Opt. Express 15(24) 15854-15862 (2007)

High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe

Aaron D. Aguirre, Juergen Sawinski, Shu-Wei Huang, Chao Zhou, Winfried Denk, and James G. Fujimoto
Opt. Express 18(5) 4222-4239 (2010)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (6)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription