In this paper quantitative imaging of biological cells using high-resolution full-field optical coherence microscopy (FF-OCM) is reported. The FF-OCM was realized using a swept-source system, a Mirau interferometer, and a CCD camera (a two-dimensional detection unit). A Mirau-interferometric objective lens was used to generate the interferometric signal. The signal was analyzed by a Fourier analysis technique. Optically sectioned amplitude images and a quantitative phase map of biological cells such as onion skin and red blood cells (RBCs) are demonstrated. Further, the refractive index profile of the RBCs is also presented. For the Mirau objective, the experimentally achieved axial and transverse resolution of the present system are 3.8 and , respectively. The CCD provides parallel detection and measures enface images without X, Y, Z mechanical scanning.
© 2011 Optical Society of AmericaFull Article | PDF Article
Andrei B. Vakhtin, Daniel J. Kane, William R. Wood, and Kristen A. Peterson
Appl. Opt. 42(34) 6953-6958 (2003)
S. Murali, K. S. Lee, and J. P Rolland
Opt. Express 15(24) 15854-15862 (2007)
Aaron D. Aguirre, Juergen Sawinski, Shu-Wei Huang, Chao Zhou, Winfried Denk, and James G. Fujimoto
Opt. Express 18(5) 4222-4239 (2010)