Abstract

Strong scattering in the optical paths can be proactively exploited for determining light propagation and focusing through turbid media and ultimately improve optical imaging and light manipulation capabilities. The use of light shapers together with strongly scattering structures enables the production of foci confined in the nanoscale or on the other hand may provide significantly enhanced fields of view. Exploiting this concept, we introduce ad hoc engineered scattering structures, by direct femtosecond laser writing in the bulk of glasses, presenting isotropy in one dimension, and together with precisely phase-shaped illuminating light we produce submicrometer thin light sheets at user-defined positions. Our approach permits us to focus light of different wavelengths onto the same defined position without moving any optical element and correcting for chromatic aberrations. Furthermore, we demonstrate that natural biological tissues presenting isotropy in one dimension can also be used for generating light sheets directly in the biological sample.

© 2016 Optical Society of America

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References

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    [Crossref]
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    [Crossref]
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    [Crossref]
  7. I. M. Vellekoop, A. Lagendijk, and A. P. Mosk, Nat. Photonics 4, 320 (2010).
  8. R. Horstmeyer, H. Ruan, and C. Yang, Nat. Photonics 9, 563 (2015).
    [Crossref]
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    [Crossref]
  10. Y. Choi, T. D. Yang, C. Fang-Yen, P. Kang, K. J. Lee, R. R. Dasari, M. S. Feld, and W. Choi, Phys. Rev. Lett. 107, 023902 (2011).
    [Crossref]
  11. D. Di Battista, G. Zacharakis, and M. Leonetti, Sci. Rep. 5, 17406 (2015).
    [Crossref]
  12. H. Yu, J. Park, K. Lee, J. Yoon, K. Kim, S. Lee, and Y. K. Park, Curr. Appl. Phys. 15, 632 (2015).
    [Crossref]
  13. R. Di Leonardo and S. Bianchi, Opt. Express 19, 247 (2011).
    [Crossref]
  14. S. A. Goorden, M. Horstmann, A. P. Mosk, B. Škorić, and P. W. Pinkse, Optica 1, 421 (2014).
    [Crossref]
  15. P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. Stelzer, Science 322, 1065 (2008).
    [Crossref]
  16. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, Science 305, 1007 (2004).
    [Crossref]
  17. Z. Yang, H. Downie, E. Rozbicki, L. X. Dupuy, and M. P. MacDonald, Opt. Express 21, 16239 (2013).
    [Crossref]
  18. D. M. Shotton, J. Cell Sci. 94, 175 (1989).
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    [Crossref]
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    [Crossref]
  21. S. Saghafi, K. Becker, C. Hahn, and H. U. Dodt, J. Biophoton. 7, 117 (2014).
    [Crossref]
  22. F. O. Fahrbach and A. Rohrbach, Opt. Express 18, 24229 (2010).
    [Crossref]
  23. N. Yu and F. Capasso, Nat. Mater. 13, 139 (2014).
    [Crossref]
  24. F. Aieta, M. A. Kats, P. Genevet, and F. Capasso, Science 347, 1342 (2015).
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  25. Q. Wang, E. T. Rogers, B. Gholipour, C. M. Wang, G. Yuan, J. Teng, and N. I. Zheludev, Nat. Photonics 10, 60 (2016).
    [Crossref]
  26. H. Zhang, D. Di Battista, G. Zacharakis, and S. Tzortzakis, Appl. Phys. Lett. 108, 211107 (2016) [109, 039901 (2016)].
    [Crossref]
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    [Crossref]
  29. E. De Tommasi, A. C. De Luca, L. Lavanga, P. Dardano, M. De Stefano, L. De Stefano, C. Langella, I. Rendina, K. Dholakia, and M. Mazilu, Opt. Express 22, 27214 (2014).
    [Crossref]
  30. S. Psilodimitrakopoulos, I. Amat-Roldan, P. Loza-Alvarez, and D. Artigas, Biomed. Opt. Express 3, 2681 (2012).
    [Crossref]
  31. M. J. Fehrenbach and T. Popowics, Illustrated Dental Embryology, Histology, and Anatomy (Elsevier, 2015).

2016 (4)

Q. Wang, E. T. Rogers, B. Gholipour, C. M. Wang, G. Yuan, J. Teng, and N. I. Zheludev, Nat. Photonics 10, 60 (2016).
[Crossref]

H. Zhang, D. Di Battista, G. Zacharakis, and S. Tzortzakis, Appl. Phys. Lett. 108, 211107 (2016) [109, 039901 (2016)].
[Crossref]

D. Wilding, P. Pozzi, O. Soloviev, G. Vdovin, C. J. Sheppard, and M. Verhaegen, Opt. Lett. 41, 1205 (2016).
[Crossref]

V. Parigi, E. Perros, G. Binard, C. Bourdillon, A. Maître, R. Carminati, V. Krachmalnicoff, and Y. De Wilde, Opt. Express 24, 7019 (2016).
[Crossref]

2015 (4)

F. Aieta, M. A. Kats, P. Genevet, and F. Capasso, Science 347, 1342 (2015).
[Crossref]

R. Horstmeyer, H. Ruan, and C. Yang, Nat. Photonics 9, 563 (2015).
[Crossref]

D. Di Battista, G. Zacharakis, and M. Leonetti, Sci. Rep. 5, 17406 (2015).
[Crossref]

H. Yu, J. Park, K. Lee, J. Yoon, K. Kim, S. Lee, and Y. K. Park, Curr. Appl. Phys. 15, 632 (2015).
[Crossref]

2014 (5)

N. Yu and F. Capasso, Nat. Mater. 13, 139 (2014).
[Crossref]

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, Nat. Methods 11, 541 (2014).
[Crossref]

S. Saghafi, K. Becker, C. Hahn, and H. U. Dodt, J. Biophoton. 7, 117 (2014).
[Crossref]

E. De Tommasi, A. C. De Luca, L. Lavanga, P. Dardano, M. De Stefano, L. De Stefano, C. Langella, I. Rendina, K. Dholakia, and M. Mazilu, Opt. Express 22, 27214 (2014).
[Crossref]

S. A. Goorden, M. Horstmann, A. P. Mosk, B. Škorić, and P. W. Pinkse, Optica 1, 421 (2014).
[Crossref]

2013 (1)

2012 (3)

2011 (3)

E. G. van Putten, D. Akbulut, J. Bertolotti, W. L. Vos, A. Lagendijk, and A. P. Mosk, Phys. Rev. Lett. 106, 193905 (2011).
[Crossref]

Y. Choi, T. D. Yang, C. Fang-Yen, P. Kang, K. J. Lee, R. R. Dasari, M. S. Feld, and W. Choi, Phys. Rev. Lett. 107, 023902 (2011).
[Crossref]

R. Di Leonardo and S. Bianchi, Opt. Express 19, 247 (2011).
[Crossref]

2010 (3)

V. Ntziachristos, Nat. Methods 7, 603 (2010).
[Crossref]

F. O. Fahrbach and A. Rohrbach, Opt. Express 18, 24229 (2010).
[Crossref]

I. M. Vellekoop, A. Lagendijk, and A. P. Mosk, Nat. Photonics 4, 320 (2010).

2009 (1)

B. Huang, M. Bates, and X. Zhuang, Annu. Rev. Biochem. 78, 993 (2009).
[Crossref]

2008 (1)

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. Stelzer, Science 322, 1065 (2008).
[Crossref]

2004 (1)

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, Science 305, 1007 (2004).
[Crossref]

1989 (1)

D. M. Shotton, J. Cell Sci. 94, 175 (1989).

1873 (1)

E. Abbe, Arch. Mikroskop. Anat. 9, 413 (1873).
[Crossref]

Abbe, E.

E. Abbe, Arch. Mikroskop. Anat. 9, 413 (1873).
[Crossref]

Aieta, F.

F. Aieta, M. A. Kats, P. Genevet, and F. Capasso, Science 347, 1342 (2015).
[Crossref]

Akbulut, D.

E. G. van Putten, D. Akbulut, J. Bertolotti, W. L. Vos, A. Lagendijk, and A. P. Mosk, Phys. Rev. Lett. 106, 193905 (2011).
[Crossref]

Amat-Roldan, I.

Artigas, D.

Bates, M.

B. Huang, M. Bates, and X. Zhuang, Annu. Rev. Biochem. 78, 993 (2009).
[Crossref]

Becker, K.

S. Saghafi, K. Becker, C. Hahn, and H. U. Dodt, J. Biophoton. 7, 117 (2014).
[Crossref]

Bertolotti, J.

E. G. van Putten, D. Akbulut, J. Bertolotti, W. L. Vos, A. Lagendijk, and A. P. Mosk, Phys. Rev. Lett. 106, 193905 (2011).
[Crossref]

Bianchi, S.

Binard, G.

Bourdillon, C.

Capasso, F.

F. Aieta, M. A. Kats, P. Genevet, and F. Capasso, Science 347, 1342 (2015).
[Crossref]

N. Yu and F. Capasso, Nat. Mater. 13, 139 (2014).
[Crossref]

Carminati, R.

Choi, W.

Y. Choi, T. D. Yang, C. Fang-Yen, P. Kang, K. J. Lee, R. R. Dasari, M. S. Feld, and W. Choi, Phys. Rev. Lett. 107, 023902 (2011).
[Crossref]

Choi, Y.

Y. Choi, T. D. Yang, C. Fang-Yen, P. Kang, K. J. Lee, R. R. Dasari, M. S. Feld, and W. Choi, Phys. Rev. Lett. 107, 023902 (2011).
[Crossref]

Cižmár, T.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, Nat. Methods 11, 541 (2014).
[Crossref]

Coll-Lladó, C.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, Nat. Methods 11, 541 (2014).
[Crossref]

Dalgarno, H. I.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, Nat. Methods 11, 541 (2014).
[Crossref]

Dardano, P.

Dasari, R. R.

Y. Choi, T. D. Yang, C. Fang-Yen, P. Kang, K. J. Lee, R. R. Dasari, M. S. Feld, and W. Choi, Phys. Rev. Lett. 107, 023902 (2011).
[Crossref]

De Luca, A. C.

De Stefano, L.

De Stefano, M.

De Tommasi, E.

De Wilde, Y.

Del Bene, F.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, Science 305, 1007 (2004).
[Crossref]

Dholakia, K.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, Nat. Methods 11, 541 (2014).
[Crossref]

E. De Tommasi, A. C. De Luca, L. Lavanga, P. Dardano, M. De Stefano, L. De Stefano, C. Langella, I. Rendina, K. Dholakia, and M. Mazilu, Opt. Express 22, 27214 (2014).
[Crossref]

Di Battista, D.

H. Zhang, D. Di Battista, G. Zacharakis, and S. Tzortzakis, Appl. Phys. Lett. 108, 211107 (2016) [109, 039901 (2016)].
[Crossref]

D. Di Battista, G. Zacharakis, and M. Leonetti, Sci. Rep. 5, 17406 (2015).
[Crossref]

Di Leonardo, R.

Dodt, H. U.

S. Saghafi, K. Becker, C. Hahn, and H. U. Dodt, J. Biophoton. 7, 117 (2014).
[Crossref]

Downie, H.

Dupuy, L. X.

Fahrbach, F. O.

Fang-Yen, C.

Y. Choi, T. D. Yang, C. Fang-Yen, P. Kang, K. J. Lee, R. R. Dasari, M. S. Feld, and W. Choi, Phys. Rev. Lett. 107, 023902 (2011).
[Crossref]

Fehrenbach, M. J.

M. J. Fehrenbach and T. Popowics, Illustrated Dental Embryology, Histology, and Anatomy (Elsevier, 2015).

Feld, M. S.

Y. Choi, T. D. Yang, C. Fang-Yen, P. Kang, K. J. Lee, R. R. Dasari, M. S. Feld, and W. Choi, Phys. Rev. Lett. 107, 023902 (2011).
[Crossref]

Ferrier, D. E.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, Nat. Methods 11, 541 (2014).
[Crossref]

Fink, M.

A. P. Mosk, A. Lagendijk, G. Lerosey, and M. Fink, Nat. Photonics 6, 283 (2012).
[Crossref]

Genevet, P.

F. Aieta, M. A. Kats, P. Genevet, and F. Capasso, Science 347, 1342 (2015).
[Crossref]

Gholipour, B.

Q. Wang, E. T. Rogers, B. Gholipour, C. M. Wang, G. Yuan, J. Teng, and N. I. Zheludev, Nat. Photonics 10, 60 (2016).
[Crossref]

Goorden, S. A.

Gualda, E. J.

Gunn-Moore, F. J.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, Nat. Methods 11, 541 (2014).
[Crossref]

Hahn, C.

S. Saghafi, K. Becker, C. Hahn, and H. U. Dodt, J. Biophoton. 7, 117 (2014).
[Crossref]

Horstmann, M.

Horstmeyer, R.

R. Horstmeyer, H. Ruan, and C. Yang, Nat. Photonics 9, 563 (2015).
[Crossref]

Huang, B.

B. Huang, M. Bates, and X. Zhuang, Annu. Rev. Biochem. 78, 993 (2009).
[Crossref]

Huisken, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, Science 305, 1007 (2004).
[Crossref]

Kang, P.

Y. Choi, T. D. Yang, C. Fang-Yen, P. Kang, K. J. Lee, R. R. Dasari, M. S. Feld, and W. Choi, Phys. Rev. Lett. 107, 023902 (2011).
[Crossref]

Kats, M. A.

F. Aieta, M. A. Kats, P. Genevet, and F. Capasso, Science 347, 1342 (2015).
[Crossref]

Keller, P. J.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. Stelzer, Science 322, 1065 (2008).
[Crossref]

Kim, K.

H. Yu, J. Park, K. Lee, J. Yoon, K. Kim, S. Lee, and Y. K. Park, Curr. Appl. Phys. 15, 632 (2015).
[Crossref]

Krachmalnicoff, V.

Lagendijk, A.

A. P. Mosk, A. Lagendijk, G. Lerosey, and M. Fink, Nat. Photonics 6, 283 (2012).
[Crossref]

E. G. van Putten, D. Akbulut, J. Bertolotti, W. L. Vos, A. Lagendijk, and A. P. Mosk, Phys. Rev. Lett. 106, 193905 (2011).
[Crossref]

I. M. Vellekoop, A. Lagendijk, and A. P. Mosk, Nat. Photonics 4, 320 (2010).

Langella, C.

Lavanga, L.

Lee, K.

H. Yu, J. Park, K. Lee, J. Yoon, K. Kim, S. Lee, and Y. K. Park, Curr. Appl. Phys. 15, 632 (2015).
[Crossref]

Lee, K. J.

Y. Choi, T. D. Yang, C. Fang-Yen, P. Kang, K. J. Lee, R. R. Dasari, M. S. Feld, and W. Choi, Phys. Rev. Lett. 107, 023902 (2011).
[Crossref]

Lee, S.

H. Yu, J. Park, K. Lee, J. Yoon, K. Kim, S. Lee, and Y. K. Park, Curr. Appl. Phys. 15, 632 (2015).
[Crossref]

Leonetti, M.

D. Di Battista, G. Zacharakis, and M. Leonetti, Sci. Rep. 5, 17406 (2015).
[Crossref]

Lerosey, G.

A. P. Mosk, A. Lagendijk, G. Lerosey, and M. Fink, Nat. Photonics 6, 283 (2012).
[Crossref]

Licea-Rodriguez, J.

Loza-Alvarez, P.

MacDonald, M. P.

Maître, A.

Mayer, J.

Mazilu, M.

Mosk, A. P.

S. A. Goorden, M. Horstmann, A. P. Mosk, B. Škorić, and P. W. Pinkse, Optica 1, 421 (2014).
[Crossref]

A. P. Mosk, A. Lagendijk, G. Lerosey, and M. Fink, Nat. Photonics 6, 283 (2012).
[Crossref]

E. G. van Putten, D. Akbulut, J. Bertolotti, W. L. Vos, A. Lagendijk, and A. P. Mosk, Phys. Rev. Lett. 106, 193905 (2011).
[Crossref]

I. M. Vellekoop, A. Lagendijk, and A. P. Mosk, Nat. Photonics 4, 320 (2010).

Ntziachristos, V.

V. Ntziachristos, Nat. Methods 7, 603 (2010).
[Crossref]

Nylk, J.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, Nat. Methods 11, 541 (2014).
[Crossref]

Olarte, O. E.

Palero, J. A.

Parigi, V.

Park, J.

H. Yu, J. Park, K. Lee, J. Yoon, K. Kim, S. Lee, and Y. K. Park, Curr. Appl. Phys. 15, 632 (2015).
[Crossref]

Park, Y. K.

H. Yu, J. Park, K. Lee, J. Yoon, K. Kim, S. Lee, and Y. K. Park, Curr. Appl. Phys. 15, 632 (2015).
[Crossref]

Perros, E.

Pinkse, P. W.

Popowics, T.

M. J. Fehrenbach and T. Popowics, Illustrated Dental Embryology, Histology, and Anatomy (Elsevier, 2015).

Pozzi, P.

Psilodimitrakopoulos, S.

Rangel-Rojo, R.

Rendina, I.

Ripoll, J.

J. Ripoll, Principles of Diffuse Light Propagation (World Scientific, 2012).

Rocha-Mendoza, I.

Rogers, E. T.

Q. Wang, E. T. Rogers, B. Gholipour, C. M. Wang, G. Yuan, J. Teng, and N. I. Zheludev, Nat. Photonics 10, 60 (2016).
[Crossref]

Rohrbach, A.

Rozbicki, E.

Ruan, H.

R. Horstmeyer, H. Ruan, and C. Yang, Nat. Photonics 9, 563 (2015).
[Crossref]

Saghafi, S.

S. Saghafi, K. Becker, C. Hahn, and H. U. Dodt, J. Biophoton. 7, 117 (2014).
[Crossref]

Schmidt, A. D.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. Stelzer, Science 322, 1065 (2008).
[Crossref]

Sharpe, J.

Sheppard, C. J.

Shotton, D. M.

D. M. Shotton, J. Cell Sci. 94, 175 (1989).

Škoric, B.

Soloviev, O.

Stelzer, E. H.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. Stelzer, Science 322, 1065 (2008).
[Crossref]

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, Science 305, 1007 (2004).
[Crossref]

Swoger, J.

Teng, J.

Q. Wang, E. T. Rogers, B. Gholipour, C. M. Wang, G. Yuan, J. Teng, and N. I. Zheludev, Nat. Photonics 10, 60 (2016).
[Crossref]

Tyson, R.

R. Tyson, Principles of Adaptive Optics (CRC Press, 2010).

Tzortzakis, S.

H. Zhang, D. Di Battista, G. Zacharakis, and S. Tzortzakis, Appl. Phys. Lett. 108, 211107 (2016) [109, 039901 (2016)].
[Crossref]

van Putten, E. G.

E. G. van Putten, D. Akbulut, J. Bertolotti, W. L. Vos, A. Lagendijk, and A. P. Mosk, Phys. Rev. Lett. 106, 193905 (2011).
[Crossref]

Vdovin, G.

Vellekoop, I. M.

I. M. Vellekoop, A. Lagendijk, and A. P. Mosk, Nat. Photonics 4, 320 (2010).

Verhaegen, M.

Vettenburg, T.

T. Vettenburg, H. I. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, Nat. Methods 11, 541 (2014).
[Crossref]

Vos, W. L.

E. G. van Putten, D. Akbulut, J. Bertolotti, W. L. Vos, A. Lagendijk, and A. P. Mosk, Phys. Rev. Lett. 106, 193905 (2011).
[Crossref]

Wang, C. M.

Q. Wang, E. T. Rogers, B. Gholipour, C. M. Wang, G. Yuan, J. Teng, and N. I. Zheludev, Nat. Photonics 10, 60 (2016).
[Crossref]

Wang, Q.

Q. Wang, E. T. Rogers, B. Gholipour, C. M. Wang, G. Yuan, J. Teng, and N. I. Zheludev, Nat. Photonics 10, 60 (2016).
[Crossref]

Wilding, D.

Wittbrodt, J.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. Stelzer, Science 322, 1065 (2008).
[Crossref]

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, Science 305, 1007 (2004).
[Crossref]

Yang, C.

R. Horstmeyer, H. Ruan, and C. Yang, Nat. Photonics 9, 563 (2015).
[Crossref]

Yang, T. D.

Y. Choi, T. D. Yang, C. Fang-Yen, P. Kang, K. J. Lee, R. R. Dasari, M. S. Feld, and W. Choi, Phys. Rev. Lett. 107, 023902 (2011).
[Crossref]

Yang, Z.

Yoon, J.

H. Yu, J. Park, K. Lee, J. Yoon, K. Kim, S. Lee, and Y. K. Park, Curr. Appl. Phys. 15, 632 (2015).
[Crossref]

Yu, H.

H. Yu, J. Park, K. Lee, J. Yoon, K. Kim, S. Lee, and Y. K. Park, Curr. Appl. Phys. 15, 632 (2015).
[Crossref]

Yu, N.

N. Yu and F. Capasso, Nat. Mater. 13, 139 (2014).
[Crossref]

Yuan, G.

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Supplementary Material (1)

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Figures (4)

Fig. 1.
Fig. 1. Anisotropic photonic glass (APG) is fabricated in the bulk of the glass by direct laser writing as illustrated in panel (a). The distribution of the lattice structure on the x–z plane is depicted in (b). An optical transmission micrograph of a fabricated APG is shown in panel (c). (d) Speckle pattern at the back of the photonic lattice: the isotropy along the y axis in the APG results in an elongated speckle pattern. (e) Correlation function Corr(r) of the pattern along the direction x (green solid line) and the direction y (blue solid line).

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Fig. 2.
Fig. 2. Cylindrical lens generates a light sheet at its focus f1=150  mm. In panel (a) we show the free space configuration. In (b) we introduce the APG at 148.9 mm from the lens. We shape the beam by addressing to the SLM phase masks composed of parallel stripes to conserve the system isotropy. If segmented masks as in panel (c) are used for the optimization process, the isotropy is lost and a conventional round focus [lower side of panel (c)] is generated.

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Fig. 3.
Fig. 3. Images (a)–(c) show, respectively, light sheets generated at 488, 532, and 594 nm laser source wavelengths. The intensity profiles along the x axis [blue curve in panel (d)] and along the y axis [red curve in panel (e)] provide the light-sheet width (w¯X=0.9  μm) and length (w¯y=21  μm). In (f) we show the light sheet along the z axis, and we measured the FWHM of its intensity profile (wz=67  μm) as shown in plot (g).

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Fig. 4.
Fig. 4. Adaptive focusing through a DT. In (a) a thin DT shows parallel tubules running along the dentinal section. In (b) a thick DT forms a perfect natural APG. The light trespassing the DT forms elongated speckles isotropically oriented along one direction given by the tubules orientation as shown in (c). ϑ is the angular distance between the orientation of the grains and the vertical camera axis. To maintain the isotropy the SLM is rotated at an angle ϑ as illustrated in the sketch (d). Sketch (e) shows the light sheet at the end of the process.

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