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Dual-focus coherent anti-Stokes Raman scattering microscopy using a compact two-beam fiber laser source

Fisseha Bekele Legesse, Tobias Meyer, Sandro Heuke, Thomas Gottschall, Torbjörn Pascher, Jens Limpert, Andreas Tünnermann, Michael Schmitt, and Jürgen Popp

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Abstract

We have developed a dual-focus coherent anti-Stokes Raman scattering (CARS) microscope based on a dual output, compact fiber laser source. The underlying concepts of time-multiplexed, two-beam scanning and demultiplexed detection that we already employed for second-harmonic generation are here naturally extended for CARS microscopy. The layout of a robust, all-fiber laser source was reconfigured to provide two outputs, each containing the two colors necessary for the CARS process. The utilization of the design for simultaneously imaging two laterally or axially separated fields of view and, thus, inherently speeding up the image acquisition process, is demonstrated on human artery tissue samples.

© 2017 Optical Society of America

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