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Digital holographic microscopy in label-free analysis of cultured cells’ response to photodynamic treatment

A. V. Belashov, A. A. Zhikhoreva, T. N. Belyaeva, E. S. Kornilova, N. V. Petrov, A. V. Salova, I. V. Semenova, and O. S. Vasyutinskii

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Abstract

A methodology providing noninvasive monitoring and evaluation of the effect of photodynamic treatment on live cells in vitro is presented. Variations in morphological characteristics of cells in the course and after treatment are recorded by means of digital holographic microscopy. High-precision measurements of phase shift gained by probe radiation in HeLa and human endometrial mesenchymal stem cell cultures demonstrate for the first time changes of their volume occurred in response to treatment.

© 2016 Optical Society of America

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Supplementary Material (1)

NameDescription
Visualization 1: MPEG (651 KB)      Visualization 1 presents the alteration dynamics in HeLa cells induced by 7-min irradiation and recorded in both optical and holographic microscopes.

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