Abstract

We present experimental confirmation of the predicted improved depth-discrimination properties of confocal microscopy in which detail outside the focal plane is rejected from the image. This optical sectioning is of direct importance to the microscopy of thick biological tissue. It is shown that detail that is imaged efficiently in a confocal microscope will be in focus.

© 1981 Optical Society of America

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References

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  1. A. Atalar, C. F. Quate, H. K. Wickramasinghe, Appl. Phys. Lett. 31, 791 (1977).
    [CrossRef]
  2. M. Born, E. Wolf, Principles of Optics (Pergamon, London, 1975).
  3. C. J. R. Sheppard, T. Wilson, Opt. Lett. 3, 115 (1978).
    [CrossRef] [PubMed]

1978

1977

A. Atalar, C. F. Quate, H. K. Wickramasinghe, Appl. Phys. Lett. 31, 791 (1977).
[CrossRef]

Atalar, A.

A. Atalar, C. F. Quate, H. K. Wickramasinghe, Appl. Phys. Lett. 31, 791 (1977).
[CrossRef]

Born, M.

M. Born, E. Wolf, Principles of Optics (Pergamon, London, 1975).

Quate, C. F.

A. Atalar, C. F. Quate, H. K. Wickramasinghe, Appl. Phys. Lett. 31, 791 (1977).
[CrossRef]

Sheppard, C. J. R.

Wickramasinghe, H. K.

A. Atalar, C. F. Quate, H. K. Wickramasinghe, Appl. Phys. Lett. 31, 791 (1977).
[CrossRef]

Wilson, T.

Wolf, E.

M. Born, E. Wolf, Principles of Optics (Pergamon, London, 1975).

Appl. Phys. Lett.

A. Atalar, C. F. Quate, H. K. Wickramasinghe, Appl. Phys. Lett. 31, 791 (1977).
[CrossRef]

Opt. Lett.

Other

M. Born, E. Wolf, Principles of Optics (Pergamon, London, 1975).

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Figures (4)

Fig. 1
Fig. 1

(a) Type 1 scanning microscope. (b) Type 2 (or confocal) scanning microscope.

Fig. 2
Fig. 2

Depth discrimination in the confocal microscope.

Fig. 3
Fig. 3

(a) Conventional scanning microscope image. (b) Confocal-scanning microscope image.

Fig. 4
Fig. 4

(a) Conventional scanning microscope image. (b) Confocal scanning microscope image.

Equations (1)

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