Abstract
We propose a lensfree on-chip microscopy approach for wide-field quantitative phase imaging (QPI) based on wavelength scanning. Unlike previous methods, we found that a relatively large-range wavelength diversity not only provides information to overcome spatial aliasing of the image sensor but also creates sufficient diffraction variations that can be used to achieve motion-free, pixel-super-resolved phase recovery. Based on an iterative phase retrieval and pixel-super-resolution technique, the proposed wavelength-scanning approach uses only eight undersampled holograms to achieve a half-pitch lateral resolution of 691 nm across a large field-of-view of $29.85\;{{\rm mm}^2}$, surpassing 2.41 times the theoretical Nyquist–Shannon sampling resolution limit imposed by the pixel size of the sensor (1.67 µm). We confirmed the effectiveness of this technique in QPI and resolution enhancement by measuring the benchmark quantitative phase microscopy target. We also showed that this method can track HeLa cell growth within an incubator, revealing cellular morphologies and subcellular dynamics of a large cell population over an extended period of time.
© 2021 Optical Society of America
Full Article | PDF ArticleMore Like This
Jialin Zhang, Qian Chen, Jiaji Li, Jiasong Sun, and Chao Zuo
Opt. Lett. 43(15) 3714-3717 (2018)
Yang Chen, Xuejuan Wu, Linpeng Lu, Jiahao Wei, Yumin Wu, Qian Chen, and Chao Zuo
Opt. Lett. 47(23) 6061-6064 (2022)
Yi Xiao, Shiyuan Wei, Shaolong Xue, Cuifang Kuang, Anli Yang, Maoliang Wei, Hongtao Lin, and Renjie Zhou
Opt. Lett. 46(19) 4785-4788 (2021)