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Virtual histopathology with ultraviolet scattering and photoacoustic remote sensing microscopy

Nathaniel J. M. Haven, Matthew T. Martell, Brendyn D. Cikaluk, Brendon S. Restall, Ewan McAlister, Sveta Silverman, Lashan Peiris, Jean Deschenes, Xingyu Li, and Roger J. Zemp

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Abstract

Realistic label-free virtual histopathology has been a long sought-after goal not yet achieved with current methods. Here, we introduce high-resolution hematoxylin and eosin (H&E)-like virtual histology of unstained human breast lumpectomy specimen sections using ultraviolet scattering-augmented photoacoustic remote sensing microscopy. Together with a colormap-matching algorithm based on blind stain separation from a reference true H&E image, we are able to produce virtual H&E images of unstained tissues with close concordance to true H&E-stained sections, with promising diagnostic utility.

© 2021 Optical Society of America

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