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Frequency interferometric localization microscopy

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Abstract

We propose a new super-resolution microscopy, named frequency interferometric localization microscopy (FILM). FILM is implemented by incorporating a Michelson interferometer into wide-field fluorescence microscope, which introduces coherence time as a new auxiliary axis to obtain the spectral information of individual fluorophores. After the time–wavelength transformation, the homogeneous linewidth of individual fluorophores can be isolated from the inhomogeneous broadening distribution of the fluorophore ensemble. Thus, the nearby fluorophores with a distinguishable central wavelength can be separated in the frequency domain and localized with accuracy beyond the diffraction limit. The principle of the method, experimental schematics, and reconstruction algorithm are numerically demonstrated. With properly prepared fluorophores, FILM has the potential to reach, in principle, molecular-scale spatial resolution.

© 2021 Optical Society of America

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Supplementary Material (2)

NameDescription
Supplement 1       The document shows some details of FILM.
Visualization 1       The movie displays a three-dimensional data cube slice-by-slice in (x,y,?) coordinate system. With the varying frequency, the intensity distribution of the image also changes, which proves the feasibility of separating adjacent emitters in frequency domain.

Data Availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

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Figures (4)

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Equations (2)

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