Abstract

In this Letter, we propose spatially incoherent multiwavelength three-dimensional (3D) microscopy that exploits holographic multiplexing and is based on computational coherent superposition (CCS). The proposed microscopy generates spatially incoherent wavelength-multiplexed self-interference holograms with a multiband-pass filter and spatially and temporally incoherent light diffracted from specimens. Selective extractions of 3D spatial information at multiple wavelengths from the holograms are realized using the CCS scheme. We constructed fully mechanical-motion-free holographic multiwavelength 3D microscopy systems and conducted experiments to demonstrate the microscopy.

© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

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S. Murata and N. Yasuda, Opt. Laser Technol. 32, 567 (2000).
[Crossref]

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T.-C. Poon, K. Doh, B. Schilling, M. Wu, K. Shinoda, and Y. Suzuki, Opt. Eng. 34, 1338 (1995).
[Crossref]

1992 (1)

1988 (1)

1967 (1)

J. W. Goodman and R. W. Lawrence, Appl. Phys. Lett. 11, 77 (1967).
[Crossref]

Alexandre, D.

Arai, Y.

T. Tahara, R. Otani, K. Omae, T. Gotohda, Y. Arai, and Y. Takaki, Opt. Express 25, 11157 (2017).
[Crossref]

T. Tahara, T. Kanno, Y. Arai, and T. Ozawa, J. Opt. 19, 065705 (2017).
[Crossref]

T. Tahara, R. Mori, S. Kikunaga, Y. Arai, and Y. Takaki, Opt. Lett. 40, 2810 (2015).
[Crossref]

T. Tahara, R. Mori, Y. Arai, and Y. Takaki, J. Opt. 17, 125707 (2015).
[Crossref]

T. Tahara, S. Kikunaga, and Y. Arai, “Digital holography apparatus and digital holography method,” Japan patentJP6308594 (11 April2018).

Barada, D.

Brodoline, A.

Brooker, G.

N. Siegel, V. Lupashin, B. Storrie, and G. Brooker, Nat. Photonics 10, 802 (2016).
[Crossref]

J. Rosen and G. Brooker, Nat. Photonics 2, 190 (2008).
[Crossref]

J. Rosen and G. Brooker, Opt. Lett. 32, 912 (2007).
[Crossref]

Bulbul, A.

Cubedo, N.

Dandliker, R.

Dasari, R. R.

Depeursinge, C.

Y. K. Park, C. Depeursinge, and G. Popescu, Nat. Photonics 12, 578 (2018).
[Crossref]

Doh, K.

T.-C. Poon, K. Doh, B. Schilling, M. Wu, K. Shinoda, and Y. Suzuki, Opt. Eng. 34, 1338 (1995).
[Crossref]

Feld, M. S.

Goodman, J. W.

J. W. Goodman and R. W. Lawrence, Appl. Phys. Lett. 11, 77 (1967).
[Crossref]

Gotohda, T.

Grosse, M.

Hayasaki, Y.

J.-P. Liu, T. Tahara, Y. Hayasaki, and T.-C. Poon, Appl. Sci. 8, 143 (2018).
[Crossref]

T. Kiire, D. Barada, J. Sugisaka, Y. Hayasaki, and T. Yatagai, Opt. Lett. 37, 3153 (2012).
[Crossref]

Hoshiba, T.

Ikeda, T.

Imbe, M.

Indebetouw, G.

Ito, T.

Javidi, B.

Kalenkov, G. S.

S. G. Kalenkov, G. S. Kalenkov, and A. E. Shtanko, J. Opt. Soc. Am. A 36, A34 (2019).
[Crossref]

S. G. Kalenkov, G. S. Kalenkov, and A. E. Shtanko, J. Opt. Soc. Am. B 34, B49 (2017).
[Crossref]

S. G. Kalenkov, G. S. Kalenkov, and A. E. Shtanko, in Light-Energy and the Environment, OSA Technical Digest (online) (Optical Society of America, 2016), paper FTu2E.7.

Kalenkov, S. G.

S. G. Kalenkov, G. S. Kalenkov, and A. E. Shtanko, J. Opt. Soc. Am. A 36, A34 (2019).
[Crossref]

S. G. Kalenkov, G. S. Kalenkov, and A. E. Shtanko, J. Opt. Soc. Am. B 34, B49 (2017).
[Crossref]

S. G. Kalenkov, G. S. Kalenkov, and A. E. Shtanko, in Light-Energy and the Environment, OSA Technical Digest (online) (Optical Society of America, 2016), paper FTu2E.7.

Kanno, T.

T. Tahara, T. Kanno, Y. Arai, and T. Ozawa, J. Opt. 19, 065705 (2017).
[Crossref]

Kashter, Y.

Kawai, H.

Kawata, S.

Kelner, R.

Kiire, T.

Kikunaga, S.

T. Tahara, R. Mori, S. Kikunaga, Y. Arai, and Y. Takaki, Opt. Lett. 40, 2810 (2015).
[Crossref]

T. Tahara, S. Kikunaga, and Y. Arai, “Digital holography apparatus and digital holography method,” Japan patentJP6308594 (11 April2018).

Kim, M. K.

Kumar, M.

Lawrence, R. W.

J. W. Goodman and R. W. Lawrence, Appl. Phys. Lett. 11, 77 (1967).
[Crossref]

Liu, J.-P.

J.-P. Liu, T. Tahara, Y. Hayasaki, and T.-C. Poon, Appl. Sci. 8, 143 (2018).
[Crossref]

P. W. M. Tsang, J.-P. Liu, and T.-C. Poon, Optica 2, 476 (2015).
[Crossref]

Lupashin, V.

N. Siegel, V. Lupashin, B. Storrie, and G. Brooker, Nat. Photonics 10, 802 (2016).
[Crossref]

Matoba, O.

T. Tahara, X. Quan, R. Otani, Y. Takaki, and O. Matoba, Microscopy 67, 55 (2018).
[Crossref]

Minami, S.

Miura, J.

Mori, R.

T. Tahara, R. Mori, Y. Arai, and Y. Takaki, J. Opt. 17, 125707 (2015).
[Crossref]

T. Tahara, R. Mori, S. Kikunaga, Y. Arai, and Y. Takaki, Opt. Lett. 40, 2810 (2015).
[Crossref]

Mukherjee, S.

Murata, S.

S. Murata and N. Yasuda, Opt. Laser Technol. 32, 567 (2000).
[Crossref]

Naik, D. N.

Noda, T.

Ohzu, H.

Omae, K.

Osten, W.

Otani, R.

T. Tahara, X. Quan, R. Otani, Y. Takaki, and O. Matoba, Microscopy 67, 55 (2018).
[Crossref]

T. Tahara, R. Otani, K. Omae, T. Gotohda, Y. Arai, and Y. Takaki, Opt. Express 25, 11157 (2017).
[Crossref]

Ozawa, T.

T. Tahara, T. Kanno, Y. Arai, and T. Ozawa, J. Opt. 19, 065705 (2017).
[Crossref]

Park, Y. K.

Y. K. Park, C. Depeursinge, and G. Popescu, Nat. Photonics 12, 578 (2018).
[Crossref]

Pedrini, G.

Poon, T.-C.

J.-P. Liu, T. Tahara, Y. Hayasaki, and T.-C. Poon, Appl. Sci. 8, 143 (2018).
[Crossref]

P. W. M. Tsang, J.-P. Liu, and T.-C. Poon, Optica 2, 476 (2015).
[Crossref]

B. W. Schilling, T.-C. Poon, G. Indebetouw, B. Storrie, K. Shinoda, Y. Suzuki, and M. H. Wu, Opt. Lett. 22, 1506 (1997).
[Crossref]

T.-C. Poon, K. Doh, B. Schilling, M. Wu, K. Shinoda, and Y. Suzuki, Opt. Eng. 34, 1338 (1995).
[Crossref]

Popescu, G.

Y. K. Park, C. Depeursinge, and G. Popescu, Nat. Photonics 12, 578 (2018).
[Crossref]

G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, Opt. Lett. 31, 775 (2006).
[Crossref]

Prongue, D.

Quan, X.

T. Tahara, X. Quan, R. Otani, Y. Takaki, and O. Matoba, Microscopy 67, 55 (2018).
[Crossref]

Rai, M. R.

Rawat, N.

Rosen, J.

Sato, Y.

Schilling, B.

T.-C. Poon, K. Doh, B. Schilling, M. Wu, K. Shinoda, and Y. Suzuki, Opt. Eng. 34, 1338 (1995).
[Crossref]

Schilling, B. W.

Shimobaba, T.

Shinoda, K.

B. W. Schilling, T.-C. Poon, G. Indebetouw, B. Storrie, K. Shinoda, Y. Suzuki, and M. H. Wu, Opt. Lett. 22, 1506 (1997).
[Crossref]

T.-C. Poon, K. Doh, B. Schilling, M. Wu, K. Shinoda, and Y. Suzuki, Opt. Eng. 34, 1338 (1995).
[Crossref]

Shtanko, A. E.

S. G. Kalenkov, G. S. Kalenkov, and A. E. Shtanko, J. Opt. Soc. Am. A 36, A34 (2019).
[Crossref]

S. G. Kalenkov, G. S. Kalenkov, and A. E. Shtanko, J. Opt. Soc. Am. B 34, B49 (2017).
[Crossref]

S. G. Kalenkov, G. S. Kalenkov, and A. E. Shtanko, in Light-Energy and the Environment, OSA Technical Digest (online) (Optical Society of America, 2016), paper FTu2E.7.

Siegel, N.

N. Siegel, V. Lupashin, B. Storrie, and G. Brooker, Nat. Photonics 10, 802 (2016).
[Crossref]

Storrie, B.

Sugisaka, J.

Suzuki, Y.

B. W. Schilling, T.-C. Poon, G. Indebetouw, B. Storrie, K. Shinoda, Y. Suzuki, and M. H. Wu, Opt. Lett. 22, 1506 (1997).
[Crossref]

T.-C. Poon, K. Doh, B. Schilling, M. Wu, K. Shinoda, and Y. Suzuki, Opt. Eng. 34, 1338 (1995).
[Crossref]

Tahara, T.

J.-P. Liu, T. Tahara, Y. Hayasaki, and T.-C. Poon, Appl. Sci. 8, 143 (2018).
[Crossref]

T. Tahara, X. Quan, R. Otani, Y. Takaki, and O. Matoba, Microscopy 67, 55 (2018).
[Crossref]

T. Tahara, T. Kanno, Y. Arai, and T. Ozawa, J. Opt. 19, 065705 (2017).
[Crossref]

T. Tahara, R. Otani, K. Omae, T. Gotohda, Y. Arai, and Y. Takaki, Opt. Express 25, 11157 (2017).
[Crossref]

T. Tahara, R. Mori, Y. Arai, and Y. Takaki, J. Opt. 17, 125707 (2015).
[Crossref]

T. Tahara, R. Mori, S. Kikunaga, Y. Arai, and Y. Takaki, Opt. Lett. 40, 2810 (2015).
[Crossref]

T. Tahara, S. Kikunaga, and Y. Arai, “Digital holography apparatus and digital holography method,” Japan patentJP6308594 (11 April2018).

Takaki, Y.

Takeda, M.

Takenouchi, M.

Thalmann, R.

Tsang, P. W. M.

Vijayakumar, A.

Watanabe, E.

Wu, M.

T.-C. Poon, K. Doh, B. Schilling, M. Wu, K. Shinoda, and Y. Suzuki, Opt. Eng. 34, 1338 (1995).
[Crossref]

Wu, M. H.

Yasuda, N.

S. Murata and N. Yasuda, Opt. Laser Technol. 32, 567 (2000).
[Crossref]

Yatagai, T.

Adv. Opt. Photon. (1)

Appl. Opt. (4)

Appl. Phys. Lett. (1)

J. W. Goodman and R. W. Lawrence, Appl. Phys. Lett. 11, 77 (1967).
[Crossref]

Appl. Sci. (1)

J.-P. Liu, T. Tahara, Y. Hayasaki, and T.-C. Poon, Appl. Sci. 8, 143 (2018).
[Crossref]

J. Opt. (2)

T. Tahara, T. Kanno, Y. Arai, and T. Ozawa, J. Opt. 19, 065705 (2017).
[Crossref]

T. Tahara, R. Mori, Y. Arai, and Y. Takaki, J. Opt. 17, 125707 (2015).
[Crossref]

J. Opt. Soc. Am. A (1)

J. Opt. Soc. Am. B (1)

Microscopy (1)

T. Tahara, X. Quan, R. Otani, Y. Takaki, and O. Matoba, Microscopy 67, 55 (2018).
[Crossref]

Nat. Photonics (3)

N. Siegel, V. Lupashin, B. Storrie, and G. Brooker, Nat. Photonics 10, 802 (2016).
[Crossref]

J. Rosen and G. Brooker, Nat. Photonics 2, 190 (2008).
[Crossref]

Y. K. Park, C. Depeursinge, and G. Popescu, Nat. Photonics 12, 578 (2018).
[Crossref]

Opt. Eng. (1)

T.-C. Poon, K. Doh, B. Schilling, M. Wu, K. Shinoda, and Y. Suzuki, Opt. Eng. 34, 1338 (1995).
[Crossref]

Opt. Express (3)

Opt. Laser Technol. (1)

S. Murata and N. Yasuda, Opt. Laser Technol. 32, 567 (2000).
[Crossref]

Opt. Lett. (9)

Optica (1)

Other (6)

T. Tahara, S. Kikunaga, and Y. Arai, “Digital holography apparatus and digital holography method,” Japan patentJP6308594 (11 April2018).

S. G. Kalenkov, G. S. Kalenkov, and A. E. Shtanko, in Light-Energy and the Environment, OSA Technical Digest (online) (Optical Society of America, 2016), paper FTu2E.7.

T.-C. Poon ed., Optical Scanning Holography with MATLAB (Springer, 2007).

M. K. Kim, ed., Digital Holographic Microscopy: Principles, Techniques, and Applications (Springer, 2011).

P. Picart and J.-C. Li, eds., Digital Holography (Wiley, 2013).

T.-C. Poon and J.-P. Liu, eds., Introduction to Modern Digital Holography with MATLAB (Cambridge University, 2014).

Supplementary Material (1)

NameDescription
» Visualization 1       Images reconstructed by multiwavelength incoherent digital holography with white light based on computational coherent superposition.

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Figures (6)

Fig. 1.
Fig. 1. Schematic of the proposed microscopy.
Fig. 2.
Fig. 2. Constructed optical system with high magnification. The magnification of the whole system was 80.
Fig. 3.
Fig. 3. Experimental results for a USAF1951 test target. (a) Photograph obtained with an incoherent 2D microscope. (b) One of the recorded holograms. Images reconstructed (c) with a hologram of (b) and (d) using the proposed microscopy. These were obtained by numerical refocusing on 8, ${-}{5}$ , and 3 mm depths at red, green, and blue central wavelengths, respectively. (e) represents the plots of (a) and (d). Plotted lines are indicated by green lines of (a) and (d), which corresponds to Group 9, lines 1–3 of the test target.
Fig. 4.
Fig. 4. Constructed microscope with birefringent lenses.
Fig. 5.
Fig. 5. Experimental results for HE-stained mouse kidney cells. (a)–(c) Intensity and (d)–(f) phase images on the image sensor plane. (a), (d) Red-; (b), (e) green-; and (c), (f) blue-wavelength bands. Black and white of (d)–(f) mean 0 and ${2}\pi $ [rad]. (g) One of the recorded holograms. Color-synthesized images on (h) the image sensor and (i) numerically focused planes. Bluish-violet circles in (i) indicate refocused stained nuclei; violet and white colors mean cell cytoplasm and white light generated from a halogen lamp.
Fig. 6.
Fig. 6. Experimental results for multiple depths imaging. (a) One of the wavelength-multiplexed self-interference holograms. Reconstructed images focused on (b) left and (c) right sides of the field of view. Bluish-violet circles in (b) and (c) indicate refocused stained nuclei; violet and white colors mean cell cytoplasm and white light from a halogen lamp, respectively (Visualization 1).

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