Abstract

This Letter demonstrates a method to simultaneously measure the quantitative-phase signal (QPS) of the observed specimen and the refractive index of its surrounding medium (${{n}_{m}}$) in a time-resolved manner using a micro-structured coverslip. Such coverslips, easily integrated into perfused live-cell imaging chambers, allow to use various quantitative-phase imaging techniques to achieve this dual measurement. Since QPS is crucially dependent on ${{n}_{m}}$, the measurement of the latter paves the way for its manipulation in a controlled manner leading to a QPS contrast modulation for appealing applications, including visualizing the interior of cells.

© 2020 Optical Society of America

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