Abstract

The combination of light sheet fluorescence microscopy (LSFM) and the optical clearing method can achieve fast three-dimensional high-resolution imaging. However, there is an essential contradiction between the field of view (FoV) and spatial resolution. Also, aberration and scattering still exist after tissue clearing, which seriously limits the imaging depth of LSFM. Here we propose a Schwartz modulation method and implement it in LSFM based on a quasi-Bessel beam to enlarge the imaging FoV without sacrificing its spatial resolution. The simulation results show that the FoV of the LSFM is enlarged by a factor of 1.73 compared to the Bessel beam. The capability of extremely fast decay along the optical axis makes Schwartz modulation more tolerant for scattering, indicating potential applications for deep tissue imaging. Also, the capability of sidelobe suppression effectively decreases unnecessary fluorescence excitation and photobleaching.

© 2020 Optical Society of America

Full Article  |  PDF Article
More Like This
Investigation of the extended focusing capability of the spherical aberration to enlarge the field of view in light-sheet fluorescence microscopy

Suhui Deng, Zijun Ding, Deyi Yuan, Mingping Liu, and Huilin Zhou
J. Opt. Soc. Am. A 38(1) 19-24 (2021)

Volume holographic optical element for light sheet fluorescence microscopy

Ting-Yu Hsieh, Sunil Vyas, Jui-Ching Wu, and Yuan Luo
Opt. Lett. 45(23) 6478-6481 (2020)

Light sheet fluorescence microscopy using axi-symmetric binary phase filters

Suho Ryu, Baekcheon Seong, Chan-wool Lee, Min Yong Ahn, Woo Taek Kim, Kwang-Min Choe, and Chulmin Joo
Biomed. Opt. Express 11(7) 3936-3951 (2020)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (7)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics