Abstract

Observing microvasculature in its native environment provides invaluable information to understand the initiation and development of microcirculatory related diseases. However, the lack of a high-resolution three-dimensional (3D) imaging technique hinders in vivo investigation of the microvasculature. Recently, we found that the red blood cells can emit autofluorescence signals with short-wavelength two-photon excitation. In this study, we exploited this property and developed a time-resolved two-photon excitation microscopy system using a homemade 520 nm femtosecond fiber laser as the excitation source. Using this system, we could achieve intravital high-resolution 3D imaging of a microvascular network noninvasively. In a mouse tumor model, tumorous blood vessels could be observed and distinguished clearly from the normal vessels.

© 2020 Optical Society of America

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Supplementary Material (2)

NameDescription
» Visualization 1       A stack of two-photon excitation fluorescence microscopy depth-scanning images excited at 520 nm in the normal area.
» Visualization 2       A stack of two-photon excitation fluorescence microscopy depth-scanning images excited at 520 nm in the tumor area.

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