Abstract

We report a compact, cost-effective, and field-portable lensless imaging platform for quantitative microscopy. In this platform, the object is placed on top of an image sensor chip without using a lens. We use a low-cost galvo scanner to rapidly scan an unknown laser speckle pattern on the object. To address the positioning repeatability and accuracy issues, we directly recover the positional shifts of the speckle pattern based on the phase correlation of the captured images. To bypass the resolution limit set by the imager pixel size, we employ a sub-sampled ptychographic phase retrieval process to recover the complex object. We validate our approach using a resolution target, phase target, and biological sample. Our results show that accurate, high-quality complex images can be obtained from a lensless dataset with as few as $\sim 10$ images. We also demonstrate the reported approach to achieve a 6.4-mm by 4.6-mm field of view and a half-pitch resolution of 1 μm. The reported approach may provide a quantitative lensless imaging strategy for addressing point-of-care-, global-health-, and telemedicine-related challenges.

© 2019 Optical Society of America

Near-field Fourier ptychography: super-resolution phase retrieval via speckle illumination

He Zhang, Shaowei Jiang, Jun Liao, Junjing Deng, Jian Liu, Yongbing Zhang, and Guoan Zheng
Opt. Express 27(5) 7498-7512 (2019)

Super-resolved multispectral lensless microscopy via angle-tilted, wavelength-multiplexed ptychographic modulation

Pengming Song, Ruihai Wang, Jiakai Zhu, Tianbo Wang, Zichao Bian, Zibang Zhang, Kazunori Hoshino, Michael Murphy, Shaowei Jiang, Chengfei Guo, and Guoan Zheng
Opt. Lett. 45(13) 3486-3489 (2020)

Super-resolution microscopy via ptychographic structured modulation of a diffuser

Pengming Song, Shaowei Jiang, He Zhang, Zichao Bian, Chengfei Guo, Kazunori Hoshino, and Guoan Zheng
Opt. Lett. 44(15) 3645-3648 (2019)

High-throughput lensless whole slide imaging via continuous height-varying modulation of a tilted sensor

Shaowei Jiang, Chengfei Guo, Patrick Hu, Derek Hu, Pengming Song, Tianbo Wang, Zichao Bian, Zibang Zhang, and Guoan Zheng
Opt. Lett. 46(20) 5212-5215 (2021)

Quantitative phase imaging via a cGAN network with dual intensity images captured under centrosymmetric illumination

Xiu Li, Huaiyu Qi, Shaowei Jiang, Pengming Song, Guoan Zheng, and Yongbing Zhang
Opt. Lett. 44(11) 2879-2882 (2019)