Abstract

We demonstrate dual-Airy-beam-scanning-based volumetric two-photon microscopy (TPM) with depth-resolving capability. A pair of Airy beams with opposite acceleration is used as the excitation lights to sequentially illuminate the sample, and depth information can be resolved based on the deflection of the Airy beam. The depth-resolving range of the volumetric TPM is up to 32 μm. The advantages of the depth-resolved volumetric TPM are the depth-resolving capability over Bessel-beam-based TPM and less scanning times over traditional Gaussian-beam-based TPM. The depth-resolved volumetric TPM provides a promising fast imaging tool to study the dynamics in neural biology.

© 2019 Optical Society of America

Full Article  |  PDF Article
More Like This
Volumetric two-photon microscopy with a non-diffracting Airy beam

Xiao-Jie Tan, Cihang Kong, Yu-Xuan Ren, Cora S. W. Lai, Kevin K. Tsia, and Kenneth K. Y. Wong
Opt. Lett. 44(2) 391-394 (2019)

Resolution enhancement in an extended depth of field for volumetric two-photon microscopy

Hongsen He, Cihang Kong, Ka Yan Chan, W. L. So, Hiu Ka Fok, Yu-Xuan Ren, Cora S. W. Lai, Kevin K. Tsia, and Kenneth K. Y. Wong
Opt. Lett. 45(11) 3054-3057 (2020)

Axially resolved volumetric two-photon microscopy with an extended field of view using depth localization under mirrored Airy beams

Ryan K. Y. Chan, Hongsen He, Yu-Xuan Ren, Cora S. W. Lai, Edmund Y. Lam, and Kenneth K. Y. Wong
Opt. Express 28(26) 39563-39573 (2020)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription