Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Quantitative phase imaging with molecular vibrational sensitivity

Abstract

Quantitative phase imaging (QPI) quantifies the sample-specific optical-phase-delay enabling objective studies of optically transparent specimens such as biological samples but lacks chemical sensitivity, limiting its application to a morphology-based diagnosis. We present wide-field molecular vibrational (MV) microscopy realized in the framework of QPI utilizing a mid-infrared (MIR) photothermal effect. Our technique provides MIR spectroscopic performance comparable to that of a conventional infrared spectrometer in the molecular fingerprint region of 14501640cm1 and realizes wide-field molecular imaging of a silica-polystyrene bead mixture over a 100μm×100μm area at 1 frame per second with the spatial resolution of 430 nm and 2–3 orders of magnitude lower fluence of 10pJ/μm2 compared to other high-speed label-free molecular imaging methods, reducing photodamages to the sample. With a high-energy MIR pulse source, our technique could enable high-speed, label-free, simultaneous, and in situ acquisition of quantitative morphology and MV contrast, providing new insights for studies of optically transparent complex dynamics.

© 2019 Optical Society of America

Full Article  |  PDF Article
More Like This
Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect

Miu Tamamitsu, Keiichiro Toda, Hiroyuki Shimada, Takaaki Honda, Masaharu Takarada, Kohki Okabe, Yu Nagashima, Ryoichi Horisaki, and Takuro Ideguchi
Optica 7(4) 359-366 (2020)

Sensitivity evaluation of quantitative phase imaging: a study of wavelength shifting interferometry

Shichao Chen, Chengshuai Li, and Yizheng Zhu
Opt. Lett. 42(6) 1088-1091 (2017)

Quantitative phase imaging via a cGAN network with dual intensity images captured under centrosymmetric illumination

Xiu Li, Huaiyu Qi, Shaowei Jiang, Pengming Song, Guoan Zheng, and Yongbing Zhang
Opt. Lett. 44(11) 2879-2882 (2019)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved