Abstract

High-resolution nonlinear fluorescence (NF) microscopy that utilizes repetitive stimulated transition due to the saturation of stimulated emission caused by two-color continuous-wave lasers was developed. The resulting NF signal, detected via the lock-in technique, is produced by the multiplicative combination of incident beams, which results in an improvement of the optical resolution. The proposed method is demonstrated to have a three-dimensional optical resolution superior to that of conventional NF microscopy. The results of biological imaging reveal the feasibility and superiority of the proposed method.

© 2019 Optical Society of America

Creation of a three-dimensional spherical fluorescence spot for super-resolution microscopy using a two-color annular hybrid wave plate

Yoshinori Iketaki, Hiroshi Kumagai, Kornel Jahn, and Nandor Bokor
Opt. Lett. 40(6) 1057-1060 (2015)

High-resolution multiphoton microscopy with a low-power continuous wave laser pump

Xiang-Dong Chen, Shen Li, Bo Du, Yang Dong, Ze-Hao Wang, Guang-Can Guo, and Fang-Wen Sun
Opt. Lett. 43(4) 699-702 (2018)

Saturated two-photon excitation fluorescence microscopy with core-ring illumination

Ryosuke Oketani, Atsushi Doi, Nicholas I. Smith, Yasunori Nawa, Satoshi Kawata, and Katsumasa Fujita
Opt. Lett. 42(3) 571-574 (2017)

Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser

Kyu Young Han and Taekjip Ha
Opt. Lett. 40(11) 2653-2656 (2015)

Ratiometric photon reassignment based on fluorescence lifetime to improve resolution in pulse STED microscopy

Shaocong Liu, Zhimin Zhang, Yubing Han, Lu Yang, Cuifang Kuang, and Xu Liu
Opt. Lett. 46(13) 3304-3307 (2021)