Abstract

Endogenous and exogenous fluorescence emission from biological samples encodes complementary information. Here we report, to the best of our knowledge, the first results from an optical imaging platform with interleaved excitation and detection of exogenous and endogenous fluorescence from tissue samples using a single flexible multimode fiber that delivers the excitation beam and collects the emitted light. A custom-built reflective optical chopper wheel with synchronized rotation temporally multiplexes an autofluorescence lifetime imaging apparatus with an intensity-based fluorescence module tailored to imaging green fluorescent protein. We demonstrate the functionality of such platform imaging dyes of varying fluorescence signatures and resolving cellularized areas on bio-engineered tissue constructs.

© 2019 Optical Society of America

Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber

Benjamin E. Sherlock, Cai Li, Xiangnan Zhou, Alba Alfonso-Garcia, Julien Bec, Diego Yankelevich, and Laura Marcu
Opt. Lett. 44(9) 2302-2305 (2019)

Multimodal optical coherence tomography and fluorescence lifetime imaging with interleaved excitation sources for simultaneous endogenous and exogenous fluorescence

Sebina Shrestha, Michael J. Serafino, Jesus Rico-Jimenez, Jesung Park, Xi Chen, Siqin Zhaorigetu, Brian L. Walton, Javier A. Jo, and Brian E. Applegate
Biomed. Opt. Express 7(9) 3184-3197 (2016)

Simultaneous, label-free, multispectral fluorescence lifetime imaging and optical coherence tomography using a double-clad fiber

Benjamin E. Sherlock, Jennifer E. Phipps, Julien Bec, and Laura Marcu
Opt. Lett. 42(19) 3753-3756 (2017)

Focusing light in biological tissue through a multimode optical fiber: refractive index matching

Raphaël Turcotte, Carla C. Schmidt, Nigel J. Emptage, and Martin J. Booth
Opt. Lett. 44(10) 2386-2389 (2019)

Automatic segmentation of intravital fluorescence microscopy images by K-means clustering of FLIM phasors

Yide Zhang, Takashi Hato, Pierre C. Dagher, Evan L. Nichols, Cody J. Smith, Kenneth W. Dunn, and Scott S. Howard
Opt. Lett. 44(16) 3928-3931 (2019)