Abstract

We developed a high-speed two-photon optical ribbon imaging system, which combines galvo-mirrors for an arbitrary curve scan on a lateral plane and a tunable acoustic gradient-index lens for a 100 kHz–1 MHz axial scan. The system provides micrometer/millisecond spatiotemporal resolutions, which enable continuous readout of functional dynamics from small and densely packed neurons in a living adult Drosophila brain. Compared to sparse sampling techniques, the ribbon imaging modality avoids motion artifacts. Combined with a Drosophila anatomical connectome database, which is the most complete among all model animals, this technique paves the way toward establishing whole-brain functional connectome.

© 2019 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Optical properties of adult Drosophila brains in one-, two-, and three-photon microscopy

Kuo-Jen Hsu, Yen-Yin Lin, Ann-Shyn Chiang, and Shi-Wei Chu
Biomed. Opt. Express 10(4) 1627-1637 (2019)

Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens

Benjamin F. Grewe, Fabian F. Voigt, Marcel van ’t Hoff, and Fritjof Helmchen
Biomed. Opt. Express 2(7) 2035-2046 (2011)

Motion quantification during multi-photon functional imaging in behaving animals

Lingjie Kong, Justin P. Little, and Meng Cui
Biomed. Opt. Express 7(9) 3686-3695 (2016)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (1)

NameDescription
» Visualization 1       Ribbon-scan images of mushroom body in a Drosophila brain, corresponding to Fig. 3b in the main text. It shows that the whole MB structures stay stationary without noticeable movements throughout the stimulation.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription