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Stain-free subcellular-resolution astrocyte imaging using third-harmonic generation

M. S. Pochechuev, A. A. Lanin, I. V. Kelmanson, D. S. Bilan, D. A. Kotova, A. S. Chebotarev, V. Tarabykin, A. B. Fedotov, V. V. Belousov, and A. M. Zheltikov

Abstract

We demonstrate stain-free, high-contrast, subcellular-resolution imaging of astroglial cells using epi-detected third-harmonic generation (THG). The astrocyte-imaging capability of THG is verified by colocalizing THG images with fluorescence images of astrocytes expressing a genetically encodable fluorescent reporter. We show that THG imaging with an optimized point-spread function can reliably detect significant subcellular features of astrocytes, including cell nuclei, as well as the soma shape and boundaries.

© 2019 Optical Society of America

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Supplementary Material (1)

NameDescription
Visualization 1       Stacks of TPEF and THG images taken by scanning the focus of the laser beam along the z-axis through layers V–VI in rat’s cortex.

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