Abstract

We show that compressive sensing calculations are very efficient to reconstruct in 3D sparse objects whose 2D hologram has been recorded by digital holographic microscopy. The method is well adapted to image small scattering objects moving within a larger motionless object. This situation corresponds to red blood cells (RBCs) circulating in the vascular system of a zebrafish (Danio rerio) larva. RBC positions are imaged in 3D from a single hologram, while the RBC trajectories, i.e., the perfused blood vessels, are imaged from a sequence of holograms. With respect to previous work (Donnarumma et al., Opt. Express 24, 26887 (2016) [CrossRef]  ), we get a gain of 500 in calculation speed.

© 2019 Optical Society of America

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Supplementary Material (4)

NameDescription
» Visualization 1       360° rotation of an image showing the instantaneous 3D positions of red blood cells in the perfused vessels of a 5-days zebrafish larva from one camera frame.
» Visualization 2       360° rotation of an image showing the instantaneous 3D positions of red blood cells in the perfused vessels of a 5-days zebrafish larva during several camera frames.
» Visualization 3       360° rotation of an image obtained by averaging on 100 camera frames showing the perfused vessels of a 5-days zebrafish larva.
» Visualization 4       360° rotation of an image showing the perfused vessels of a 5-days zebrafish larva and the instantaneous positions of the red blood cells during several camera frames.

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