Abstract

We demonstrate a method for quantitative phase (QP) imaging without 2π ambiguity using a spectral domain phase microscopy system. The method is capable of QP measurement of a large dynamic range with a high sensitivity. We determine an integer multiple of 2π to correct wrapped phases by calculating the phase shift difference between the detected interference fringe and simulated fringes. The presented method is quantitatively verified by measuring the vibration generated by a piezo linear stage and mapping the surface topography of a slanted mirror. QP imaging of red blood cells is also performed to demonstrate the method’s capacity and application in biological imaging.

© 2018 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Passive vibration compensation in scanning white-light interferometry

Stanislav Tereschenko, Peter Lehmann, Lisa Zellmer, and Angelika Brueckner-Foit
Appl. Opt. 55(23) 6172-6182 (2016)

Quadrature wavelength scanning interferometry

Giuseppe Moschetti, Alistair Forbes, Richard K. Leach, Xiang Jiang, and Daniel O’Connor
Appl. Opt. 55(20) 5332-5340 (2016)

High-dynamic-range quantitative phase imaging with spectral domain phase microscopy

Jun Zhang, Bin Rao, Lingfeng Yu, and Zhongping Chen
Opt. Lett. 34(21) 3442-3444 (2009)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (5)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription