Abstract

Volumetric imaging tools that are simple to adopt, flexible, and robust are in high demand in the field of neuroscience, where the ability to image neurons and their networks with high spatiotemporal resolution is essential. Using an axially elongated focus approximating a Bessel beam, in combination with two-photon fluorescence microscopy, has proven successful at such an endeavor. Here, we demonstrate three-photon fluorescence imaging with an axially extended Bessel focus. We use an axicon-based module that allowed for the generation of Bessel foci of varying numerical apertures and axial lengths, and apply this volumetric imaging tool to image mouse brain slices and for in vivo imaging of the mouse brain.

© 2018 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Visualization of brain circuits using two-photon fluorescence micro-optical sectioning tomography

Ting Zheng, Zhongqing Yang, Anan Li, Xiaohua Lv, Zhenqiao Zhou, Xiaojun Wang, Xiaoli Qi, Shiwei Li, Qingming Luo, Hui Gong, and Shaoqun Zeng
Opt. Express 21(8) 9839-9850 (2013)

50 Hz volumetric functional imaging with continuously adjustable depth of focus

Rongwen Lu, Masashi Tanimoto, Minoru Koyama, and Na Ji
Biomed. Opt. Express 9(4) 1964-1976 (2018)

In vivo two-photon microscopy of the hippocampus using glass plugs

Mary Grace M. Velasco and Michael J. Levene
Biomed. Opt. Express 5(6) 1700-1708 (2014)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription