Abstract
Volumetric imaging tools that are simple to adopt, flexible, and robust are in high demand in the field of neuroscience, where the ability to image neurons and their networks with high spatiotemporal resolution is essential. Using an axially elongated focus approximating a Bessel beam, in combination with two-photon fluorescence microscopy, has proven successful at such an endeavor. Here, we demonstrate three-photon fluorescence imaging with an axially extended Bessel focus. We use an axicon-based module that allowed for the generation of Bessel foci of varying numerical apertures and axial lengths, and apply this volumetric imaging tool to image mouse brain slices and for in vivo imaging of the mouse brain.
© 2018 Optical Society of America
Full Article | PDF ArticleOSA Recommended Articles
Ting Zheng, Zhongqing Yang, Anan Li, Xiaohua Lv, Zhenqiao Zhou, Xiaojun Wang, Xiaoli Qi, Shiwei Li, Qingming Luo, Hui Gong, and Shaoqun Zeng
Opt. Express 21(8) 9839-9850 (2013)
Rongwen Lu, Masashi Tanimoto, Minoru Koyama, and Na Ji
Biomed. Opt. Express 9(4) 1964-1976 (2018)
Mary Grace M. Velasco and Michael J. Levene
Biomed. Opt. Express 5(6) 1700-1708 (2014)