Abstract

We present a new external off-axis holographic module that doubles the acquired complex wavefront field of view, based on using both holographic flipping and multiplexing. In contrast to previous designs, this design does not require spatial filtering (no pinhole or lenses) to create the reference beam externally. In addition, the overlap area between the fields of view, as well as the off-axis angle between the sample and reference beams, can be fully controlled. As we demonstrate experimentally, this approach is useful for quantitative phase microscopy of extended stationary and dynamic samples, such as cancer cells during rapid flow and beating cardiomyocytes.

© 2018 Optical Society of America

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Corrections

7 November 2018: Typographical corrections were made to paragraphs 1 and 3 of page 5545, and paragraph 2 of page 5546.


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Supplementary Material (3)

NameDescription
» Visualization 1       Double field of view quantitative phase imaging of a flowing cancer cell.
» Visualization 2       Double field of view quantitative phase imaging of a beating cardiomyocyte monolayer
» Visualization 3       Double field of view quantitative phase imaging of a beating cardiomyocyte monolayer

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