Abstract

A new type of functional optical microscope system called three-dimensional (3D) stimulation and imaging-based functional optical microscopy (SIFOM) is proposed, to the best of our knowledge. SIFOM can precisely stimulate user-defined targeted biological cells and can simultaneously record the volumetric fluorescence distribution in a single acquisition. Precise and simultaneous stimulation of fluorescent-labeled biological cells is achieved by multiple 3D spots generated by digital holograms displayed on a phase-mode spatial light modulator. Single-shot 3D acquisition of the fluorescence distribution is accomplished by common-path off-axis incoherent digital holographic microscopy in which a diffraction grating with a focusing lens is displayed on another phase-mode spatial light modulator. The effectiveness of the proposed functional microscope system was verified in experiments using fluorescent microbeads and human lung cancer cells located at various defocused positions. The system can be used for manipulating the states of cells in optogenetics.

© 2018 Optical Society of America

Full Article  |  PDF Article
More Like This
Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells

Yoav N. Nygate, Gyanendra Singh, Itay Barnea, and Natan T. Shaked
Opt. Lett. 43(11) 2587-2590 (2018)

Three-dimensional super-resolved live cell imaging through polarized multi-angle TIRF

Cheng Zheng, Guangyuan Zhao, Wenjie Liu, Youhua Chen, Zhimin Zhang, Luhong Jin, Yingke Xu, Cuifang Kuang, and Xu Liu
Opt. Lett. 43(7) 1423-1426 (2018)

Image recovery from defocused 2D fluorescent images in multimodal digital holographic microscopy

Xiangyu Quan, Osamu Matoba, and Yasuhiro Awatsuji
Opt. Lett. 42(9) 1796-1799 (2017)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (3)

NameDescription
» Visualization 1       A movie showing focused images of microbeads with selective illumination.
» Visualization 2       A movie showing holograms of microbeads defocused in z-direction.
» Visualization 3       A movie showing reconstructed images of microbeads from holograms.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (6)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription