Abstract

The implementation of a numerical dark field illumination to obtain reconstructions with enhanced contrast in digital lensless holographic (DLHM) microscopy is presented. In this proposal, an opaque circular stop is numerically inserted in the light path between the sample and hologram plane. The contrast of the reconstructed holograms is increased at least 46% in comparison with that obtained by the regular reconstruction methods. This proposal has been validated using experimental holograms of a paramecium in water and a section of the head of a Drosophila melanogaster fly recorded in DLHM.

© 2018 Optical Society of America

Full Article  |  PDF Article
More Like This
Preprocessing in digital lensless holographic microscopy for intensity reconstructions with enhanced contrast

Heberley Tobon, Carlos Trujillo, and Jorge Garcia-Sucerquia
Appl. Opt. 60(4) A215-A221 (2021)

Automatic method for focusing biological specimens in digital lensless holographic microscopy

Carlos A. Trujillo and Jorge Garcia-Sucerquia
Opt. Lett. 39(9) 2569-2572 (2014)

Cooperative execution of auto-focusing metrics in digital lensless holographic microscopy for internal-structured samples

Carlos Trujillo and Jorge Garcia-Sucerquia
Appl. Opt. 56(21) 5877-5882 (2017)

View More...

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (6)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription