Abstract

Label-free particle analysis is a powerful tool in chemical, pharmaceutical, and cosmetic industries as well as in basic sciences, but its throughput is significantly lower than that of fluorescence-based counterparts. Here we present a label-free single-particle analyzer based on broadband dual-comb coherent Raman scattering spectroscopy operating at a spectroscopic scan rate of 10 kHz. As a proof-of-concept demonstration, we perform broadband coherent anti-Stokes Raman scattering measurements of polystyrene microparticles flowing on an acoustofluidic chip at a high throughput of >1000 particles per second. This high-throughput label-free particle analyzer has the potential for high-precision statistical analysis of a large number of microparticles including biological cells.

© 2018 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Stimulated Raman scattering flow cytometry for label-free single-particle analysis

Chi Zhang, Kai-Chih Huang, Bartek Rajwa, Junjie Li, Shiqi Yang, Haonan Lin, Chien-sheng Liao, Gregory Eakins, Shihuan Kuang, Valery Patsekin, J. Paul Robinson, and Ji-Xin Cheng
Optica 4(1) 103-109 (2017)

Spectral focusing dual-comb coherent anti-Stokes Raman spectroscopic imaging

Kun Chen, Tao Wu, Tao Chen, Haoyun Wei, Honglei Yang, Tian Zhou, and Yan Li
Opt. Lett. 42(18) 3634-3637 (2017)

Label-free multimodal coherent anti-Stokes Raman scattering analysis of microparticles in unconstrained microfluidics

K. O’Dwyer, R. Mouras, A. A. Mani, D. Rice, M. Gleeson, N. Liu, S. A. M. Tofail, and C. Silien
Appl. Opt. 57(22) E32-E36 (2018)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription