Abstract

Neural activity leads to alterations in cerebral blood flow (CBF), i.e., neurovascular regulation, which is implicated in brain physiology and pathology. Here a method for simultaneous imaging of CBF and spatially selective photoactivation of neural activity is proposed. CBF was obtained by laser speckle contrast imaging, and a liquid-crystal spatial light modulator (LC-SLM) was used to generate photoactivation patterns targeting designated locations in the cortex. Animal experiments stimulating defined cortical regions of VGAT-ChR2 and wild-type mice were conducted, and experiments with low-intensity stimulation were performed to investigate the influence of background light produced by the LC-SLM.

© 2018 Optical Society of America

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