Abstract

We present a three-color femtosecond Er/Yb:fiber laser enabling highly specific and standardized nonlinear optical manipulation of live cells. The system simultaneously provides bandwidth-limited 80-fs pulses with identical intensity envelope centered at wavelengths of 515, 775, and 1035 nm in the focus of a confocal microscope. We achieve this goal by combining high-order dispersion control via, for example, chirped fiber Bragg gratings with proper bandwidth management in each nonlinear conversion step. Wavelength-selective and noninterfering induction of deoxyribonucleic acid (DNA) photoproducts and DNA strand breaks, as well as fluorescence photoactivation of a photoactivatable green fluorescent protein (PA-GFP)–histone fusion protein, are demonstrated. The capability to introduce different types of DNA lesions and perform photoswitching experiments in a selective manner is essential for quantitative studies on DNA repair and chromatin dynamics.

© 2018 Optical Society of America

Sub-10-nm imaging of nucleic acids using spectroscopic intrinsic-contrast photon-localization optical nanoscopy (SICLON)

Adam Eshein, Yue Li, Biqin Dong, Luay M. Almassalha, John E. Chandler, The-Quyen Nguyen, Karl A. Hujsak, Vinayak P. Dravid, Cheng Sun, Hao F. Zhang, and Vadim Backman
Opt. Lett. 43(23) 5817-5820 (2018)

Two-photon microscopy with a frequency-doubled fully fusion-spliced fiber laser at 1840  nm

C.-H. Hage, J.-T. Gomes, S. M. Bardet, G. Granger, M. Jossent, L. Lavoute, D. Gaponov, and S. Fevrier
Opt. Lett. 43(20) 5098-5101 (2018)

Characterizing chromatin packing scaling in whole nuclei using interferometric microscopy

Aya Eid, Adam Eshein, Yue Li, Ranya Virk, David Van Derway, Di Zhang, Allen Taflove, and Vadim Backman
Opt. Lett. 45(17) 4810-4813 (2020)

Enhancement of the lasing efficiency of vitamin B₂ in a highly polar organic solvent via DNA-lipid complex

Woohyun Jung, Byung Joo Kong, Seongjin Hong, Taeoh Kim, and Kyunghwan Oh
Opt. Lett. 43(16) 4021-4024 (2018)

Highly sensitive selectively coated photonic crystal fiber-based plasmonic sensor

Ahmmed A. Rifat, Firoz Haider, Rajib Ahmed, Ghafour Amouzad Mahdiraji, F. R. Mahamd Adikan, and Andrey E. Miroshnichenko
Opt. Lett. 43(4) 891-894 (2018)