Abstract

Stimulated Raman scattering (SRS) is a powerful, label-free imaging technique that holds significant potential for medical imaging. To allow chemical specificity and minimize spectral distortion in the imaging of live species, a high-speed multiplex SRS imaging platform is needed. By combining a spectral focusing excitation technique with a rapid acousto-optic delay line, we demonstrate a hyperspectral SRS imaging platform capable of measuring a 3-dB spectral window of 200  cm1 within 12.8 μs with a scan rate of 30 KHz. We present hyperspectral images of a mixture of two different microsphere polymers as well as live fungal cells mixed with human blood.

© 2017 Optical Society of America

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Supplementary Material (1)

NameDescription
» Visualization 1: AVI (7397 KB)      Dynamics of Fungi and lipid droplets, MCR concentration map (left, colored), sum of all spectral channels at each pixel (right, grayscale).

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