Abstract

We propose full-field functional optical angiography for a live biological specimen based on the absorption intensity fluctuation modulation (AIFM) effect. Because of the difference in absorption between red blood cells (RBCs) and the background tissue under low-coherence light illumination, the moving RBCs, which discontinuously pass though the capillary vessels, generate an AIFM effect. This effect offers high contrast of absorption imaging and sensitivity of low-coherence interference between RBCs and the background tissue. It is used to distinguish the signal of RBCs from that of the background tissue. The averaged and real-time modulation depths are computed to obtain full-field label-free optical angiography and measure blood flow velocity simultaneously. The AIFM method could potentially be applied to study the physiological mechanisms of blood circulation systems of near-transparent live biologic samples.

© 2017 Optical Society of America

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References

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Supplementary Material (7)

NameDescription
» Visualization 1: MOV (6959 KB)      Raw image under the illumination of laser
» Visualization 2: MOV (6919 KB)      IMD image under the illumination of laser
» Visualization 3: MOV (7254 KB)      Raw image under the illumination of white light
» Visualization 4: MOV (6804 KB)      IMD image under the illumination of white light
» Visualization 5: MOV (6942 KB)      Raw image under the illumination of low-coherence light
» Visualization 6: MOV (6807 KB)      IMD image under the illumination of low-coherence light
» Visualization 7: MOV (14527 KB)      IMD image under the illumination of low-coherence light

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Figures (4)

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Equations (5)

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