Abstract

We demonstrated resolution improvement in two-photon excitation microscopy by combining saturated excitation (SAX) of fluorescence and pupil manipulation. We theoretically estimated the resolution improvement and the sidelobe effect in the point spread function with various pupil designs and found that the combination of SAX and core-ring illumination can effectively enhance the spatial resolution in 3D and suppress sidelobe artifacts. The experimental demonstration shows that the proposed technique is effective for observation with a depth of 100 μm in a tissue phantom and can be applied to 3D observations of tissue samples with higher spatial resolution than conventional two-photon excitation microscopy.

© 2017 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Description of deep saturated excitation multiphoton microscopy for super-resolution imaging

Genevieve Vigil, Yide Zhang, Aamir Khan, and Scott Howard
J. Opt. Soc. Am. A 34(7) 1217-1223 (2017)

Image formation in image scanning microscopy, including the case of two-photon excitation

Colin J. R. Sheppard, Marco Castello, Giorgio Tortarolo, Giuseppe Vicidomini, and Alberto Diaspro
J. Opt. Soc. Am. A 34(8) 1339-1350 (2017)

Two-photon excitation fluorescence microscopy with a high depth of field using an axicon

Pascal Dufour, Michel Piché, Yves De Koninck, and Nathalie McCarthy
Appl. Opt. 45(36) 9246-9252 (2006)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription