Abstract

The advent of 4π microscopy broke the conventional optical resolution limit in the axial direction of the microscope. In combination with fluorescence microscopy, it broadened the knowledge of cell biology at the expense of perturbing the samples with extrinsic fluorescent labels. In contrast, Raman microscopy acquires the molecular fingerprint of the sample without the need of extrinsic labels, and therefore improving its resolution can make an even greater impact. Here, we take advantage of the improved axial resolution of a 4π configuration to form a 4π Raman microscope. With this microscope, we independently and simultaneously analyzed different nanolayers in a multilayer stack. We identified their chemical composition and retrieved their relative subwavelength optical separation with a precision of 6 nm.

© 2017 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Generation of uniformly oriented in-plane magnetization with near-unity purity in 4π microscopy

Sicong Wang, Yaoyu Cao, and Xiangping Li
Opt. Lett. 42(23) 5050-5053 (2017)

Dual-focus coherent anti-Stokes Raman scattering microscopy using a compact two-beam fiber laser source

Fisseha Bekele Legesse, Tobias Meyer, Sandro Heuke, Thomas Gottschall, Torbjörn Pascher, Jens Limpert, Andreas Tünnermann, Michael Schmitt, and Jürgen Popp
Opt. Lett. 42(2) 183-186 (2017)

Simultaneous two-color stimulated Raman scattering microscopy by adding a fiber amplifier to a 2 ps OPO-based SRS microscope

Wenlong Yang, Ang Li, Yuanzhen Suo, Fa-Ke Lu, and X. Sunney Xie
Opt. Lett. 42(3) 523-526 (2017)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription