Abstract

Optical visualization of pathological changes in Alzheimer’s disease (AD) can facilitate exploration of disease mechanisms and treatments. However, existing optical imaging methods have limitations on mapping pathological evolution in the whole mouse brain. Previous research indicated endogenous fluorescence contrast of senile plaques. Therefore, we develop cryo-micro-optical sectioning tomography (cryo-MOST) to capture intrinsic fluorescence distribution of senile plaques at a micrometer-level resolution in the whole brain. Validation using immunofluorescence demonstrates the capacity of cryo-MOST to visualize and distinguish senile plaques with competent sensitivity and spatial resolution. Compared with imaging in room temperature, cryo-MOST provides better signal intensity and signal-to-noise ratio. Using cryo-MOST, we obtained whole-brain coronal distribution of senile plaques in a transgenic mouse without exogenous dye. Capable of label-free brainwide visualization of Alzheimer’s pathology, cryo-MOST may be potentially useful for understanding neurodegenerative disease mechanisms and evaluating drug efficacy.

© 2017 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Optical visualization of Alzheimer’s pathology via multiphoton-excited intrinsic fluorescence and second harmonic generation

Alex C. Kwan, Karen Duff, Gunnar K. Gouras, and Watt W. Webb
Opt. Express 17(5) 3679-3689 (2009)

Intravital imaging of amyloid plaques in a transgenic mouse model using optical-resolution photoacoustic microscopy

Song Hu, Ping Yan, Konstantin Maslov, Jin-Moo Lee, and Lihong V. Wang
Opt. Lett. 34(24) 3899-3901 (2009)

Label-free imaging of atherosclerotic plaques using third-harmonic generation microscopy

David M. Small, Jason S. Jones, Irwin I. Tendler, Paul E. Miller, Andre Ghetti, and Nozomi Nishimura
Biomed. Opt. Express 9(1) 214-229 (2018)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription